|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 11, 6992-7001, March 12, 1999
From the Department of Molecular Genetics and Microbiology, State
University of New York at Stony Brook,
Stony Brook, New York 11794-5222
The highly conserved non-structural protein 2C of
picornaviruses is involved in viral genome replication and
encapsidation and in the rearrangement of intracellular structures. 2C
binds RNA, has nucleoside triphosphatase activity, and shares three motifs with superfamily III helicases. Motifs "A" and "B" are involved in nucleotide triphosphate (NTP) binding and hydrolysis, whereas a function for motif "C" has not yet been demonstrated. Poliovirus RNA replication is inhibited by millimolar concentrations of
guanidine hydrochloride (GdnHCl). Resistance and dependence to GdnHCl
map to 2C. To characterize the nucleoside triphosphatase activity of
2C, we purified poliovirus recombinant 2C fused to glutathione
S-transferase (GST-2C) from Escherichia coli.
GST-2C hydrolyzed ATP with a Km of 0.7 mM. Other NTPs, including GTP, competed with ATP for
binding to 2C but were poor substrates for hydrolysis. Mutation of
conserved residues in motif A and B abolished ATPase activity, as did
mutation of the conserved asparagine residue in motif C, an observation
indicating the involvement of this motif in ATP hydrolysis. GdnHCl at
millimolar concentrations inhibited ATP hydrolysis. Mutations in 2C
that confer poliovirus resistant to or dependent on GdnHCl increased
the tolerance to GdnHCl up to 100-fold.
Characterization of the Nucleoside Triphosphatase Activity of
Poliovirus Protein 2C Reveals a Mechanism by Which Guanidine Inhibits
Poliovirus Replication
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Arita, T. Wakita, and H. Shimizu Characterization of pharmacologically active compounds that inhibit poliovirus and enterovirus 71 infectivity J. Gen. Virol., October 1, 2008; 89(10): 2518 - 2530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. H. Chua, P. Phuektes, S. A. Sanders, P. K. Nicholls, and P. C. McMinn The molecular basis of mouse adaptation by human enterovirus 71 J. Gen. Virol., July 1, 2008; 89(7): 1622 - 1632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. De Palma, W. Heggermont, K. Lanke, B. Coutard, M. Bergmann, A.-M. Monforte, B. Canard, E. De Clercq, A. Chimirri, G. Purstinger, et al. The Thiazolobenzimidazole TBZE-029 Inhibits Enterovirus Replication by Targeting a Short Region Immediately Downstream from Motif C in the Nonstructural Protein 2C J. Virol., May 15, 2008; 82(10): 4720 - 4730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Xiao, Y. Bai, H. Xu, X. Geng, J. Chen, Y. Wang, J. Chen, and B. Li Effect of NS3 and NS5B proteins on classical swine fever virus internal ribosome entry site-mediated translation and its host cellular translation J. Gen. Virol., April 1, 2008; 89(4): 994 - 999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Belsham and P. Normann Dynamics of picornavirus RNA replication within infected cells J. Gen. Virol., February 1, 2008; 89(2): 485 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yin, Y. Liu, E. Wimmer, and A. V. Paul Complete protein linkage map between the P2 and P3 non-structural proteins of poliovirus J. Gen. Virol., August 1, 2007; 88(8): 2259 - 2267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Murray and M. L. Nibert Guanidine Hydrochloride Inhibits Mammalian Orthoreovirus Growth by Reversibly Blocking the Synthesis of Double-Stranded RNA J. Virol., May 1, 2007; 81(9): 4572 - 4584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-F. Tang, S.-Y. Yang, B.-W. Wu, J.-R. Jheng, Y.-L. Chen, C.-H. Shih, K.-H. Lin, H.-C. Lai, P. Tang, and J.-T. Horng Reticulon 3 Binds the 2C Protein of Enterovirus 71 and Is Required for Viral Replication J. Biol. Chem., February 23, 2007; 282(8): 5888 - 5898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mueller, D. Papamichail, J. R. Coleman, S. Skiena, and E. Wimmer Reduction of the Rate of Poliovirus Protein Synthesis through Large-Scale Codon Deoptimization Causes Attenuation of Viral Virulence by Lowering Specific Infectivity J. Virol., October 1, 2006; 80(19): 9687 - 9696. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-T. Jeang and V. Yedavalli Role of RNA helicases in HIV-1 replication Nucleic Acids Res., September 10, 2006; 34(15): 4198 - 4205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Teterina, E. Levenson, M. S. Rinaudo, D. Egger, K. Bienz, A. E. Gorbalenya, and E. Ehrenfeld Evidence for functional protein interactions required for poliovirus RNA replication. J. Virol., June 1, 2006; 80(11): 5327 - 5337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Samuilova, C. Krogerus, I. Fabrichniy, and T. Hyypia ATP Hydrolysis and AMP Kinase Activities of Nonstructural Protein 2C of Human Parechovirus 1 J. Virol., January 15, 2006; 80(2): 1053 - 1058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Fata-Hartley and A. C. Palmenberg Dipyridamole Reversibly Inhibits Mengovirus RNA Replication J. Virol., September 1, 2005; 79(17): 11062 - 11070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Egger and K. Bienz Intracellular location and translocation of silent and active poliovirus replication complexes J. Gen. Virol., March 1, 2005; 86(3): 707 - 718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Einav, M. Elazar, T. Danieli, and J. S. Glenn A Nucleotide Binding Motif in Hepatitis C Virus (HCV) NS4B Mediates HCV RNA Replication J. Virol., October 15, 2004; 78(20): 11288 - 11295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Banerjee, M. K. Weidman, A. Echeverri, P. Kundu, and A. Dasgupta Regulation of Poliovirus 3C Protease by the 2C Polypeptide J. Virol., September 1, 2004; 78(17): 9243 - 9256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Grubman and B. Baxt Foot-and-Mouth Disease Clin. Microbiol. Rev., April 1, 2004; 17(2): 465 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Crotty, M.-C. Saleh, L. Gitlin, O. Beske, and R. Andino The Poliovirus Replication Machinery Can Escape Inhibition by an Antiviral Drug That Targets a Host Cell Protein J. Virol., April 1, 2004; 78(7): 3378 - 3386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Teterina, M. S. Rinaudo, and E. Ehrenfeld Strand-Specific RNA Synthesis Defects in a Poliovirus with a Mutation in Protein 3A J. Virol., December 1, 2003; 77(23): 12679 - 12691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Rieder, W. Xiang, A. Paul, and E. Wimmer Analysis of the cloverleaf element in a human rhinovirus type 14/poliovirus chimera: correlation of subdomain D structure, ternary protein complex formation and virus replication J. Gen. Virol., August 1, 2003; 84(8): 2203 - 2216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Krogerus, D. Egger, O. Samuilova, T. Hyypia, and K. Bienz Replication Complex of Human Parechovirus 1 J. Virol., August 1, 2003; 77(15): 8512 - 8523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Pariente, A. Airaksinen, and E. Domingo Mutagenesis versus Inhibition in the Efficiency of Extinction of Foot-and-Mouth Disease Virus J. Virol., June 15, 2003; 77(12): 7131 - 7138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yin, A. V. Paul, E. Wimmer, and E. Rieder Functional Dissection of a Poliovirus cis-Acting Replication Element [PV-cre(2C)]: Analysis of Single- and Dual-cre Viral Genomes and Proteins That Bind Specifically to PV-cre RNA J. Virol., May 1, 2003; 77(9): 5152 - 5166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Murray and D. J. Barton Poliovirus CRE-Dependent VPg Uridylylation Is Required for Positive-Strand RNA Synthesis but Not for Negative-Strand RNA Synthesis J. Virol., April 15, 2003; 77(8): 4739 - 4750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Harris and V. R. Racaniello Changes in Rhinovirus Protein 2C Allow Efficient Replication in Mouse Cells J. Virol., April 15, 2003; 77(8): 4773 - 4780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jurgens and J. B. Flanegan Initiation of Poliovirus Negative-Strand RNA Synthesis Requires Precursor Forms of P2 Proteins J. Virol., December 20, 2002; 77(2): 1075 - 1083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Lyons, K. E. Murray, A. W. Roberts, and D. J. Barton Poliovirus 5'-Terminal Cloverleaf RNA Is Required in cis for VPg Uridylylation and the Initiation of Negative-Strand RNA Synthesis J. Virol., November 15, 2001; 75(22): 10696 - 10708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Gerber, E. Wimmer, and A. V. Paul Biochemical and Genetic Studies of the Initiation of Human Rhinovirus 2 RNA Replication: Purification and Enzymatic Analysis of the RNA-Dependent RNA Polymerase 3Dpol J. Virol., November 15, 2001; 75(22): 10969 - 10978. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Banerjee and A. Dasgupta Interaction of picornavirus 2C polypeptide with the viral negative-strand RNA J. Gen. Virol., November 1, 2001; 82(11): 2621 - 2627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Pariente, S. Sierra, P. R. Lowenstein, and E. Domingo Efficient Virus Extinction by Combinations of a Mutagen and Antiviral Inhibitors J. Virol., October 15, 2001; 75(20): 9723 - 9730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Rust, L. Landmann, R. Gosert, B. L. Tang, W. Hong, H.-P. Hauri, D. Egger, and K. Bienz Cellular COPII Proteins Are Involved in Production of the Vesicles That Form the Poliovirus Replication Complex J. Virol., October 15, 2001; 75(20): 9808 - 9818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. D. Zhao and E. Wimmer Genetic Analysis of a Poliovirus/Hepatitis C Virus Chimera: New Structure for Domain II of the Internal Ribosomal Entry Site of Hepatitis C Virus J. Virol., April 15, 2001; 75(8): 3719 - 3730. [Abstract] [Full Text]
|
|
|
|
|
|
T. Pfister and E. Wimmer Polypeptide p41 of a Norwalk-Like Virus Is a Nucleic Acid-Independent Nucleoside Triphosphatase J. Virol., February 15, 2001; 75(4): 1611 - 1619. [Abstract] [Full Text]
|
|
|
|
|
|
R. Banerjee and A. Dasgupta Specific Interaction of Hepatitis C Virus Protease/Helicase NS3 with the 3'-Terminal Sequences of Viral Positive- and Negative-Strand RNA J. Virol., February 15, 2001; 75(4): 1708 - 1721. [Abstract] [Full Text]
|
|
|
|
|
|
A. V. Paul, E. Rieder, D. W. Kim, J. H. van Boom, and E. Wimmer Identification of an RNA Hairpin in Poliovirus RNA That Serves as the Primary Template in the In Vitro Uridylylation of VPg J. Virol., November 15, 2000; 74(22): 10359 - 10370. [Abstract] [Full Text]
|
|
|
|
|
|
E. Rieder, A. V. Paul, D. W. Kim, J. H. van Boom, and E. Wimmer Genetic and Biochemical Studies of Poliovirus cis-Acting Replication Element cre in Relation to VPg Uridylylation J. Virol., November 15, 2000; 74(22): 10371 - 10380. [Abstract] [Full Text]
|
|
|
|
|
|
M. S. Marín, R. Casais, J. M. M. Alonso, and F. Parra ATP Binding and ATPase Activities Associated with Recombinant Rabbit Hemorrhagic Disease Virus 2C-Like Polypeptide J. Virol., November 15, 2000; 74(22): 10846 - 10851. [Abstract] [Full Text]
|
|
|
|
|
|
T. Ahola, J. A. den Boon, and P. Ahlquist Helicase and Capping Enzyme Active Site Mutations in Brome Mosaic Virus Protein 1a Cause Defects in Template Recruitment, Negative-Strand RNA Synthesis, and Viral RNA Capping J. Virol., October 1, 2000; 74(19): 8803 - 8811. [Abstract] [Full Text]
|
|
|
|
|
|
D. Egger, N. Teterina, E. Ehrenfeld, and K. Bienz Formation of the Poliovirus Replication Complex Requires Coupled Viral Translation, Vesicle Production, and Viral RNA Synthesis J. Virol., July 15, 2000; 74(14): 6570 - 6580. [Abstract] [Full Text]
|
|
|
|
|
|
H. Shimizu, M. Agoh, Y. Agoh, H. Yoshida, K. Yoshii, T. Yoneyama, A. Hagiwara, and T. Miyamura Mutations in the 2C Region of Poliovirus Responsible for Altered Sensitivity to Benzimidazole Derivatives J. Virol., May 1, 2000; 74(9): 4146 - 4154. [Abstract] [Full Text]
|
|
|
|
|
|
M. Klein, D. Hadaschik, H. Zimmermann, H. J. Eggers, and B. Nelsen-Salz The picornavirus replication inhibitors HBB and guanidine in the echovirus-9 system: the significance of viral protein 2C J. Gen. Virol., April 1, 2000; 81(4): 895 - 901. [Abstract] [Full Text]
|
|
|
|
|
|
T. Pfister, K. W. Jones, and E. Wimmer A Cysteine-Rich Motif in Poliovirus Protein 2CATPase Is Involved in RNA Replication and Binds Zinc In Vitro J. Virol., January 1, 2000; 74(1): 334 - 343. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |