HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Candi, E. Articles by Steinert, P. M. Search for Related Content PubMed PubMed Citation Articles by Candi, E. Articles by Steinert, P. M. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 11, 7226-7237, March 12, 1999

Transglutaminase Cross-linking Properties of the Small Proline-rich 1 Family of Cornified Cell Envelope Proteins
INTEGRATION WITH LORICRIN

From the Laboratory of Skin Biology, NIAMS, National Institutes of Health, Bethesda, Maryland 20892-2752

Small proline-rich 1 (SPR1) proteins are important for barrier function in stratified squamous epithelia. To explore their properties, we expressed in bacteria a recombinant human SPR1 protein and isolated native SPR1 proteins from cultured mouse keratinocytes. By circular dichroism, they possess no  or  structure but have some organized structure associated with their central peptide repeat domain. The transglutaminase (TGase) 1 and 3 enzymes use the SPR1 proteins as complete substrates in vitro but in different ways: head domain A sequences at the amino terminus were used preferentially for cross-linking by TGase 3, whereas those in head domain B sequences were used for cross-linking by TGase 1. The TGase 2 enzyme cross-linked SPR1 proteins poorly. Together with our data base of 141 examples of in vivo cross-links between SPRs and loricrin, this means that both TGase 1 and 3 are required for cross-linking SPR1 proteins in epithelia in vivo. Double in vitro cross-linking experiments suggest that oligomerization of SPR1 into large polymers can occur only by further TGase 1 cross-linking of an initial TGase 3 reaction. Accordingly, we propose that TGase 3 first cross-links loricrin and SPRs together to form small interchain oligomers, which are then permanently affixed to the developing CE by further cross-linking by the TGase 1 enzyme. This is consistent with the known consequences of diminished barrier function in TGase 1 deficiency models.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				S. E. Iismaa, B. M. Mearns, L. Lorand, and R. M. Graham Transglutaminases and Disease: Lessons From Genetically Engineered Mouse Models and Inherited Disorders Physiol Rev, July 1, 2009; 89(3): 991 - 1023. [Abstract] [Full Text] [PDF]

				H. H. von Horsten, S. S. Johnson, S. K. SanFrancisco, M. C. Hastert, S. M. Whelly, and G. A. Cornwall Oligomerization and Transglutaminase Cross-linking of the Cystatin CRES in the Mouse Epididymal Lumen: POTENTIAL MECHANISM OF EXTRACELLULAR QUALITY CONTROL J. Biol. Chem., November 9, 2007; 282(45): 32912 - 32923. [Abstract] [Full Text] [PDF]

				T. Cheng, K. Hitomi, I. M. J. J. van Vlijmen-Willems, G. J. de Jongh, K. Yamamoto, K. Nishi, C. Watts, T. Reinheckel, J. Schalkwijk, and P. L. J. M. Zeeuwen Cystatin M/E Is a High Affinity Inhibitor of Cathepsin V and Cathepsin L by a Reactive Site That Is Distinct from the Legumain-binding Site: A NOVEL CLUE FOR THE ROLE OF CYSTATIN M/E IN EPIDERMAL CORNIFICATION J. Biol. Chem., June 9, 2006; 281(23): 15893 - 15899. [Abstract] [Full Text] [PDF]

				J. F. Staab, Y.-S. Bahn, C.-H. Tai, P. F. Cook, and P. Sundstrom Expression of Transglutaminase Substrate Activity on Candida albicans Germ Tubes through a Coiled, Disulfide-bonded N-terminal Domain of Hwp1 Requires C-terminal Glycosylphosphatidylinositol Modification J. Biol. Chem., September 24, 2004; 279(39): 40737 - 40747. [Abstract] [Full Text] [PDF]

				Y. Tesfaigzi, P. S. Wright, and S. A. Belinsky SPRR1B overexpression enhances entry of cells into the G0 phase of the cell cycle Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L889 - L898. [Abstract] [Full Text] [PDF]

				H. Vuong, T. Patterson, P. Adiseshaiah, P. Shapiro, D. V. Kalvakolanu, and S. P. M. Reddy JNK1 and AP-1 regulate PMA-inducible squamous differentiation marker expression in Clara-like H441 cells Am J Physiol Lung Cell Mol Physiol, February 1, 2002; 282(2): L215 - L225. [Abstract] [Full Text] [PDF]

				D. Marshall, M. J. Hardman, K. M. Nield, and C. Byrne Differentially expressed late constituents of the epidermal cornified envelope PNAS, November 6, 2001; 98(23): 13031 - 13036. [Abstract] [Full Text] [PDF]

				A. Cabral, A. Sayin, S. de Winter, D. F. Fischer, S. Pavel, and C. Backendorf SPRR4, a novel cornified envelope precursor: UV-dependent epidermal expression and selective incorporation into fragile envelopes J. Cell Sci., January 11, 2001; 114(21): 3837 - 3843. [Abstract] [Full Text] [PDF]

				Y.-C. Choi, G. T. Park, T.-S. Kim, I.-N. Sunwoo, P. M. Steinert, and S.-Y. Kim Sporadic Inclusion Body Myositis Correlates with Increased Expression and Cross-linking by Transglutaminases 1 and 2 J. Biol. Chem., March 17, 2000; 275(12): 8703 - 8710. [Abstract] [Full Text] [PDF]

				J. Deng, R. Pan, and R. Wu Distinct Roles for Amino- and Carboxyl-terminal Sequences of SPRR1 Protein in the Formation of Cross-linked Envelopes of Conducting Airway Epithelial Cells J. Biol. Chem., February 25, 2000; 275(8): 5739 - 5747. [Abstract] [Full Text] [PDF]

				P. M. Steinert and L. N. Marekov Initiation of Assembly of the Cell Envelope Barrier Structure of Stratified Squamous Epithelia Mol. Biol. Cell, December 1, 1999; 10(12): 4247 - 4261. [Abstract] [Full Text]

				Z. Nemes, L. N. Marekov, and P. M. Steinert Involucrin Cross-linking by Transglutaminase 1. BINDING TO MEMBRANES DIRECTS RESIDUE SPECIFICITY J. Biol. Chem., April 16, 1999; 274(16): 11013 - 11021. [Abstract] [Full Text] [PDF]

				E. Candi, S. Oddi, A. Terrinoni, A. Paradisi, M. Ranalli, A. Finazzi-Agro, and G. Melino Transglutaminase 5 Cross-links Loricrin, Involucrin, and Small Proline-rich Proteins in Vitro J. Biol. Chem., September 7, 2001; 276(37): 35014 - 35023. [Abstract] [Full Text] [PDF]

				A. Cabral, P. Voskamp, A.-M. Cleton-Jansen, A. South, D. Nizetic, and C. Backendorf Structural Organization and Regulation of the Small Proline-rich Family of Cornified Envelope Precursors Suggest a Role in Adaptive Barrier Function J. Biol. Chem., May 25, 2001; 276(22): 19231 - 19237. [Abstract] [Full Text] [PDF]