| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 11, 7565-7569, March 12, 1999
From the Departments of ¶ Anesthesiology, ** Biochemistry,
§ Cell Biology, §§ Medicine,
The substance P receptor (SPR) is a G
protein-coupled receptor (GPCR) that plays a key role in pain
regulation. The SPR desensitizes in the continued presence of agonist,
presumably via mechanisms that implicate G protein-coupled receptor
kinases (GRKs) and
Real-time Visualization of the Cellular Redistribution of G
Protein-coupled Receptor Kinase 2 and
-Arrestin 2 during Homologous
Desensitization of the Substance P Receptor
§,
,§§§, and
Pharmacology, Howard
Hughes Medical Institute, Duke University Medical Center, Durham, North
Carolina 27710
-arrestins. The temporal relationship of these
proposed biochemical events has never been established for any GPCR
other than rhodopsin beyond the resolution provided by biochemical
assays. We investigate the real-time activation and desensitization of
the human SPR in live HEK293 cells using green fluorescent protein
conjugates of protein kinase C, GRK2, and -arrestin 2. The
translocation of protein kinase C II-green fluorescent protein to
and from the plasma membrane in response to substance P indicates that the human SPR becomes activated within seconds of agonist exposure, and
the response desensitizes within 30 s. This desensitization process coincides with a redistribution of GRK2 from the cytosol to the
plasma membrane, followed by a robust redistribution of -arrestin 2 and a profound change in cell morphology that occurs after 1 min of SPR
stimulation. These data establish a role for GRKs and -arrestins in
homologous desensitization of the SPR and provide the first visual and
temporal resolution of the sequence of events underlying homologous
desensitization of a GPCR in living cells.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. H. Oakley, J. A. Olivares-Reyes, C. C. Hudson, F. Flores-Vega, F. M. Dautzenberg, and R. L. Hauger Carboxyl-terminal and intracellular loop sites for CRF1 receptor phosphorylation and beta-arrestin-2 recruitment: a mechanism regulating stress and anxiety responses Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R209 - R222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Broccardo, G. Linari, S. Agostini, G. Amadoro, F. Carpino, M. T. Ciotti, C. Petrella, V. Petrozza, C. Severini, and G. Improta Expression of NK-1 and NK-3 tachykinin receptors in pancreatic acinar cells after acute experimental pancreatitis in rats Am J Physiol Gastrointest Liver Physiol, September 1, 2006; 291(3): G518 - G524. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Roosterman, G. S. Cottrell, F. Schmidlin, M. Steinhoff, and N. W. Bunnett Recycling and Resensitization of the Neurokinin 1 Receptor: INFLUENCE OF AGONIST CONCENTRATION AND Rab GTPASES J. Biol. Chem., July 16, 2004; 279(29): 30670 - 30679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Thiyagarajan, R. P. Stracquatanio, A. N. Pronin, D. S. Evanko, J. L. Benovic, and P. B. Wedegaertner A Predicted Amphipathic Helix Mediates Plasma Membrane Localization of GRK5 J. Biol. Chem., April 23, 2004; 279(17): 17989 - 17995. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Suo, M. Wu, B. A. Citron, G. T. Wong, and B. W. Festoff Abnormality of G-Protein-Coupled Receptor Kinases at Prodromal and Early Stages of Alzheimer's Disease: An Association with Early {beta}-Amyloid Accumulation J. Neurosci., March 31, 2004; 24(13): 3444 - 3452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Chen, M. M. Paing, and J. Trejo Termination of Protease-activated Receptor-1 Signaling by {beta}-Arrestins Is Independent of Receptor Phosphorylation J. Biol. Chem., March 12, 2004; 279(11): 10020 - 10031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Johnson, L. M. Bohn, and P. H. Taghert Drosophila CG8422 encodes a functional diuretic hormone receptor J. Exp. Biol., February 15, 2004; 207(5): 743 - 748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Johnson, L. M. Bohn, L. S. Barak, R. T. Birse, D. R. Nassel, M. G. Caron, and P. H. Taghert Identification of Drosophila Neuropeptide Receptors by G Protein-coupled Receptors-{beta}-Arrestin2 Interactions J. Biol. Chem., December 26, 2003; 278(52): 52172 - 52178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pfeiffer, S. Kirscht, R. Stumm, T. Koch, D. Wu, M. Laugsch, H. Schroder, V. Hollt, and S. Schulz Heterodimerization of Substance P and {micro}-Opioid Receptors Regulates Receptor Trafficking and Resensitization J. Biol. Chem., December 19, 2003; 278(51): 51630 - 51637. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Schmidlin, D. Roosterman, and N. W. Bunnett The third intracellular loop and carboxyl tail of neurokinin 1 and 3 receptors determine interactions with {beta}-arrestins Am J Physiol Cell Physiol, October 1, 2003; 285(4): C945 - C958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Perron, Z.-g. Chen, D. Gingras, D. J. Dupre, J. Stankova, and M. Rola-Pleszczynski Agonist-independent Desensitization and Internalization of the Human Platelet-activating Factor Receptor by Coumermycin-Gyrase B-induced Dimerization J. Biol. Chem., July 18, 2003; 278(30): 27956 - 27965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Pollok-Kopp, K. Schwarze, V. K. Baradari, and M. Oppermann Analysis of Ligand-stimulated CC Chemokine Receptor 5 (CCR5) Phosphorylation in Intact Cells Using Phosphosite-specific Antibodies J. Biol. Chem., January 17, 2003; 278(4): 2190 - 2198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. H. Crane, M. J. Williams, A. J. Nimmo, and G. P. Hamlin Estrogen-Dependent Regulation of Neurokinin 3 Receptor-Mediated Uterine Contractility in the Rat Biol Reprod, November 1, 2002; 67(5): 1480 - 1487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Schmidlin, O. Dery, N. W. Bunnett, and E. F. Grady Heterologous regulation of trafficking and signaling of G protein-coupled receptors: beta -Arrestin-dependent interactions between neurokinin receptors PNAS, March 5, 2002; 99(5): 3324 - 3329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Kirkwood, N. W. Bunnett, J. Maa, I. Castagliolo, B. Liu, N. Gerard, J. Zacks, C. Pothoulakis, and E. F. Grady Deletion of neutral endopeptidase exacerbates intestinal inflammation induced by Clostridium difficile toxin A Am J Physiol Gastrointest Liver Physiol, August 1, 2001; 281(2): G544 - G551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Willets, R. A. J. Challiss, E. Kelly, and S. R. Nahorski G Protein-Coupled Receptor Kinases 3 and 6 Use Different Pathways to Desensitize the Endogenous M3 Muscarinic Acetylcholine Receptor in Human SH-SY5Y Cells Mol. Pharmacol., August 1, 2001; 60(2): 321 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Dery, K. A. Defea, and N. W. Bunnett Protein kinase C-mediated desensitization of the neurokinin 1 receptor Am J Physiol Cell Physiol, May 1, 2001; 280(5): C1097 - C1106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Ferrari and A. Bisello Cellular Distribution of Constitutively Active Mutant Parathyroid Hormone (PTH)/PTH-Related Protein Receptors and Regulation of Cyclic Adenosine 3',5'-Monophosphate Signaling by {beta}-Arrestin2 Mol. Endocrinol., January 1, 2001; 15(1): 149 - 163. [Abstract] [Full Text]
|
|
|
|
|
|
L. S. Barak, R. H. Oakley, S. A. Laporte, and M. G. Caron Constitutive arrestin-mediated desensitization of a human vasopressin receptor mutant associated with nephrogenic diabetes insipidus PNAS, December 22, 2000; (2000) 11303698. [Abstract] [Full Text]
|
|
|
|
|
|
S. J. Mundell and J. L. Benovic Selective Regulation of Endogenous G Protein-coupled Receptors by Arrestins in HEK293 Cells J. Biol. Chem., April 21, 2000; 275(17): 12900 - 12908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Vallentin, C. Prevostel, T. Fauquier, X. Bonnefont, and D. Joubert Membrane Targeting and Cytoplasmic Sequestration in the Spatiotemporal Localization of Human Protein Kinase C alpha J. Biol. Chem., February 25, 2000; 275(8): 6014 - 6021. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Y. Vollmer, P. Alix, A. Chollet, K. Takeda, and J.-L. Galzi Subcellular Compartmentalization of Activation and Desensitization of Responses Mediated by NK2 Neurokinin Receptors J. Biol. Chem., December 31, 1999; 274(53): 37915 - 37922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bhatnagar, D. L. Willins, J. A. Gray, J. Woods, J. L. Benovic, and B. L. Roth The Dynamin-dependent, Arrestin-independent Internalization of 5-Hydroxytryptamine 2A (5-HT2A) Serotonin Receptors Reveals Differential Sorting of Arrestins and 5-HT2A Receptors during Endocytosis J. Biol. Chem., March 9, 2001; 276(11): 8269 - 8277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Hughes, H. Zhang, D. E. Logothetis, and C. H. Berlot Visualization of a Functional Galpha q-Green Fluorescent Protein Fusion in Living Cells. ASSOCIATION WITH THE PLASMA MEMBRANE IS DISRUPTED BY MUTATIONAL ACTIVATION AND BY ELIMINATION OF PALMITOYLATION SITES, BUT NOT BY ACTIVATION MEDIATED BY RECEPTORS OR AlF4- J. Biol. Chem., February 2, 2001; 276(6): 4227 - 4235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shiina, K. Arai, S. Tanabe, N. Yoshida, T. Haga, T. Nagao, and H. Kurose Clathrin Box in G Protein-coupled Receptor Kinase 2 J. Biol. Chem., August 24, 2001; 276(35): 33019 - 33026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Schmidlin, O. Dery, K. O. DeFea, L. Slice, S. Patierno, C. Sternini, E. F. Grady, and N. W. Bunnett Dynamin and Rab5a-dependent Trafficking and Signaling of the Neurokinin 1 Receptor J. Biol. Chem., June 29, 2001; 276(27): 25427 - 25437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. Naga Prasad, L. S. Barak, A. Rapacciuolo, M. G. Caron, and H. A. Rockman Agonist-dependent Recruitment of Phosphoinositide 3-Kinase to the Membrane by beta -Adrenergic Receptor Kinase 1. A ROLE IN RECEPTOR SEQUESTRATION J. Biol. Chem., May 25, 2001; 276(22): 18953 - 18959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Min, C. Galet, and M. Ascoli The Association of Arrestin-3 with the Human Lutropin/Choriogonadotropin Receptor Depends Mostly on Receptor Activation Rather than on Receptor Phosphorylation J. Biol. Chem., January 4, 2002; 277(1): 702 - 710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Oakley, S. A. Laporte, J. A. Holt, M. G. Caron, and L. S. Barak Differential Affinities of Visual Arrestin, beta Arrestin1, and beta Arrestin2 for G Protein-coupled Receptors Delineate Two Major Classes of Receptors J. Biol. Chem., May 26, 2000; 275(22): 17201 - 17210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Barak, R. H. Oakley, S. A. Laporte, and M. G. Caron Constitutive arrestin-mediated desensitization of a human vasopressin receptor mutant associated with nephrogenic diabetes insipidus PNAS, January 2, 2001; 98(1): 93 - 98. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
