HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pagano, A. Articles by Paccaud, J.-P. Search for Related Content PubMed PubMed Citation Articles by Pagano, A. Articles by Paccaud, J.-P.

J Biol Chem, Vol. 274, Issue 12, 7833-7840, March 19, 1999

Sec24 Proteins and Sorting at the Endoplasmic Reticulum

Alessandra Pagano, François Letourneur, David Garcia-Estefania^§, Jean-Louis Carpentier, Lelio Orci, and Jean-Pierre Paccaud

From the Department of Morphology, University Medical Center, ^§ Department of Zoology, Geneva University, Geneva CH-1211, Switzerland and the  Institute of Biology and Chemistry of Proteins, CNRS, Lyon, 69367 France

COPII proteins are necessary to generate secretory vesicles at the endoplasmic reticulum. In yeast, the Sec24p protein is the only COPII component in which two close orthologues have been identified. By using gene knock-out in yeast, we found that the absence of one of these Sec24 orthologues resulted in a selective secretion defect for a subset of proteins released into the medium. Data base searches revealed the existence of an entire family of Sec24-related proteins in humans, worms, flies, and plants. We identified and cloned two new human cDNAs encoding proteins homologous to yeast Sec24p, in addition to two human cDNAs already present within the data bases. The entire Sec24 family identified to date is characterized by clusters of highly conserved residues within the 2/3 carboxyl-terminal domain of all the proteins and a divergent amino terminus domain. Human (h) Sec24 orthologues co-immunoprecipitate with hSec23Ap and migrate as a complex by size exclusion chromatography. Immunofluorescence microscopy confirmed that these proteins co-localize with hSec23p and hSec13p. Together, our data suggest that in addition to its role in the shaping up of the vesicle, the Sec23-24p complex may be implicated in cargo selection and concentration.

---

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

This article has been cited by other articles:

				J. Moelleken, J. Malsam, M. J. Betts, A. Movafeghi, I. Reckmann, I. Meissner, A. Hellwig, R. B. Russell, T. Sollner, B. Brugger, et al. Differential localization of coatomer complex isoforms within the Golgi apparatus PNAS, March 13, 2007; 104(11): 4425 - 4430. [Abstract] [Full Text] [PDF]

				M. C. Stankewich, P. R. Stabach, and J. S. Morrow Human Sec31B: a family of new mammalian orthologues of yeast Sec31p that associate with the COPII coat. J. Cell Sci., March 1, 2006; 119(Pt 5): 958 - 969. [Abstract] [Full Text] [PDF]

				A. Matyash, H.-R. Chung, and H. Jackle Genome-wide Mapping of in Vivo Targets of the Drosophila Transcription Factor Kruppel J. Biol. Chem., July 16, 2004; 279(29): 30689 - 30696. [Abstract] [Full Text] [PDF]

				D. J. Stephens and R. Pepperkok Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum J. Cell Sci., July 15, 2004; 117(16): 3635 - 3644. [Abstract] [Full Text] [PDF]

				N. Fatal, L. Karhinen, E. Jokitalo, and M. Makarow Active and specific recruitment of a soluble cargo protein for endoplasmic reticulum exit in the absence of functional COPII component Sec24p J. Cell Sci., May 1, 2004; 117(9): 1665 - 1673. [Abstract] [Full Text] [PDF]

				A. Soza, A. Norambuena, J. Cancino, E. de la Fuente, P. Henklein, and A. Gonzalez Sorting Competition with Membrane-permeable Peptides in Intact Epithelial Cells Revealed Discrimination of Transmembrane Proteins Not Only at the trans-Golgi Network but Also at Pre-Golgi Stages J. Biol. Chem., April 23, 2004; 279(17): 17376 - 17383. [Abstract] [Full Text] [PDF]

				N. Fatal, T. Suntio, and M. Makarow Selective Protein Exit from Yeast Endoplasmic Reticulum in Absence of Functional COPII Coat Component Sec13p Mol. Biol. Cell, December 1, 2002; 13(12): 4130 - 4140. [Abstract] [Full Text] [PDF]

				A. Nantel, D. Dignard, C. Bachewich, D. Harcus, A. Marcil, A.-P. Bouin, C. W. Sensen, H. Hogues, M. van het Hoog, P. Gordon, et al. Transcription Profiling of Candida albicans Cells Undergoing the Yeast-to-Hyphal Transition Mol. Biol. Cell, October 1, 2002; 13(10): 3452 - 3465. [Abstract] [Full Text] [PDF]

				P. J. Espenshade, W.-P. Li, and D. Yabe Sterols block binding of COPII proteins to SCAP, thereby controlling SCAP sorting in ER PNAS, September 3, 2002; 99(18): 11694 - 11699. [Abstract] [Full Text] [PDF]

				M. C. S. Lee, S. Hamamoto, and R. Schekman Ceramide Biosynthesis Is Required for the Formation of the Oligomeric H+-ATPase Pma1p in the Yeast Endoplasmic Reticulum J. Biol. Chem., June 14, 2002; 277(25): 22395 - 22401. [Abstract] [Full Text] [PDF]

				U. Rein, U. Andag, R. Duden, H. D. Schmitt, and A. Spang ARF-GAP-mediated interaction between the ER-Golgi v-SNAREs and the COPI coat J. Cell Biol., April 29, 2002; 157(3): 395 - 404. [Abstract] [Full Text] [PDF]

				A. Adisa, F. R. Albano, J. Reeder, M. Foley, and L. Tilley Evidence for a role for a Plasmodium falciparum homologue of Sec31p in the export of proteins to the surface of malaria parasite-infected erythrocytes J. Cell Sci., March 11, 2002; 114(18): 3377 - 3386. [Abstract] [Full Text] [PDF]

				O. Nufer, S. Guldbrandsen, M. Degen, F. Kappeler, J.-P. Paccaud, K. Tani, and H.-P. Hauri Role of cytoplasmic C-terminal amino acids of membrane proteins in ER export J. Cell Sci., January 2, 2002; 115(3): 619 - 628. [Abstract] [Full Text] [PDF]

				B. L. Tang, Y. S. Ong, B. Huang, S. Wei, E. T. Wong, R. Qi, H. Horstmann, and W. Hong A Membrane Protein Enriched in Endoplasmic Reticulum Exit Sites Interacts with COPII J. Biol. Chem., October 19, 2001; 276(43): 40008 - 40017. [Abstract] [Full Text] [PDF]

				R. C. Rust, L. Landmann, R. Gosert, B. L. Tang, W. Hong, H.-P. Hauri, D. Egger, and K. Bienz Cellular COPII Proteins Are Involved in Production of the Vesicles That Form the Poliovirus Replication Complex J. Virol., October 15, 2001; 75(20): 9808 - 9818. [Abstract] [Full Text] [PDF]

				Y. Takai, T. Sasaki, and T. Matozaki Small GTP-Binding Proteins Physiol Rev, January 1, 2001; 81(1): 153 - 208. [Abstract] [Full Text] [PDF]

				Y. Shimoni, T. Kurihara, M. Ravazzola, M. Amherdt, L. Orci, and R. Schekman Lst1p and Sec24p Cooperate in Sorting of the Plasma Membrane ATPase into COPII Vesicles in Saccharomyces cerevisiae J. Cell Biol., November 20, 2000; 151(5): 973 - 984. [Abstract] [Full Text] [PDF]

				E. Belles-Boix, E. Babiychuk, M. V. Montagu, D. Inze, and S. Kushnir CEF, a Sec24 homologue of Arabidopsis thaliana, enhances the survival of yeast under oxidative stress conditions J. Exp. Bot., October 1, 2000; 51(351): 1761 - 1762. [Abstract] [Full Text] [PDF]

				B. L. Tang, T. Zhang, D. Y. H. Low, E. T. Wong, H. Horstmann, and W. Hong Mammalian Homologues of Yeast Sec31p. AN UBIQUITOUSLY EXPRESSED FORM IS LOCALIZED TO ENDOPLASMIC RETICULUM (ER) EXIT SITES AND IS ESSENTIAL FOR ER-GOLGI TRANSPORT J. Biol. Chem., April 28, 2000; 275(18): 13597 - 13604. [Abstract] [Full Text] [PDF]

				R. Peng, A. De Antoni, and D. Gallwitz Evidence for Overlapping and Distinct Functions in Protein Transport of Coat Protein Sec24p Family Members J. Biol. Chem., April 6, 2000; 275(15): 11521 - 11528. [Abstract] [Full Text] [PDF]

				T. Kurihara, S. Hamamoto, R. E. Gimeno, C. A. Kaiser, R. Schekman, and T. Yoshihisa Sec24p and Iss1p Function Interchangeably in Transport Vesicle Formation from the Endoplasmic Reticulum in Saccharomyces cerevisiae Mol. Biol. Cell, March 1, 2000; 11(3): 983 - 998. [Abstract] [Full Text]

				D. Stephens, N Lin-Marq, A Pagano, R Pepperkok, and J. Paccaud COPI-coated ER-to-Golgi transport complexes segregate from COPII in close proximity to ER exit sites J. Cell Sci., January 6, 2000; 113(12): 2177 - 2185. [Abstract] [PDF]

				H. Hauri, F Kappeler, H Andersson, and C Appenzeller ERGIC-53 and traffic in the secretory pathway J. Cell Sci., January 2, 2000; 113(4): 587 - 596. [Abstract] [PDF]

				C. F. Gilstring, M. Melin-Larsson, and P. O. Ljungdahl Shr3p Mediates Specific COPII Coatomer-Cargo Interactions Required for the Packaging of Amino Acid Permeases Into ER-derived Transport Vesicles Mol. Biol. Cell, November 1, 1999; 10(11): 3549 - 3565. [Abstract] [Full Text]

				T Suntio, A Shmelev, M Lund, and M Makarow The sorting determinant guiding Hsp150 to the COPI-independent transport pathway in yeast J. Cell Sci., January 11, 1999; 112(22): 3889 - 3898. [Abstract] [PDF]

				H. Higashio, Y. Kimata, T. Kiriyama, A. Hirata, and K. Kohno Sfb2p, a Yeast Protein Related to Sec24p, Can Function as a Constituent of COPII Coats Required for Vesicle Budding from the Endoplasmic Reticulum J. Biol. Chem., June 2, 2000; 275(23): 17900 - 17908. [Abstract] [Full Text] [PDF]

				U. Rein, U. Andag, R. Duden, H. D. Schmitt, and A. Spang ARF-GAP-mediated interaction between the ER-Golgi v-SNAREs and the COPI coat J. Cell Biol., April 29, 2002; 157(3): 395 - 404. [Abstract] [Full Text] [PDF]