| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 12, 7923-7928, March 19, 1999
§,
From the Hypotonic volume expansion of skate erythrocytes
rapidly stimulates the tyrosine phosphorylation of band 3, the membrane
protein thought to mediate the osmotically sensitive taurine efflux.
Skate erythrocytes possess numerous tyrosine kinases including p59fyn, p56lyn, pp60src, and p72syk, demonstrated by immune
complex assays measuring autocatalytic kinase activity. Inclusion of
the cytoplasmic domain of band 3 in this assay showed that only Syk and
Lyn can directly phosphorylate the cytoplasmic domain of band 3. Upon
cell volume expansion, Syk activity was increased as assessed by three
different assays (immune complex assay measuring autophosphorylation,
assay of the level of phosphotyrosine of the immunoprecipitated kinase, and assay of level of 32P in the kinase
immunoprecipitated from cells prelabeled with 32PO4 and then volume-expanded). The tyrosine
kinase Lyn was also stimulated by volume expansion, most notably when
analyzed by the latter two methods. Volume expansion stimulated a large
increase in the ability of Syk to phosphorylate band 3 at times that
coincide with the stimulation of taurine flux. The stilbene piceatannol inhibited Syk preferentially over Lyn and other tyrosine kinases and
inhibited volume-stimulated taurine efflux in a
concentration-dependent manner similar to that for the
inhibition of Syk. Two major phosphorylation peaks were detected in
tryptic digests of cdb3 separated by reverse phase HPLC. Edman
degradation demonstrated a phosphotyrosine in a YXXL motif.
In conclusion, p72syk appears to be a strong candidate as a
pivotal signal-transducing step in the volume-activated taurine efflux
in skate red cells. The level of band-3 phosphorylation may be
regulated, in addition, by a protein-tyrosine phosphatase of the 1B variety.
The Inflammatory Bowel Disease Research
Center, The University of Chicago, Chicago, Illinois 60637, ¶ Division of Biology and Medicine, Brown University, Providence,
Rhode Island 02912, and § Mount Desert Island Biological
Laboratory, Salsbury Cove, Maine 04672
This article has been cited by other articles:
|
|
 |
|
  M. W. Musch, A. B. Puffer, and L. Goldstein Volume expansion stimulates monoubiquitination and endocytosis of surface-expressed skate anion-exchanger isoform Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1657 - R1665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Musch and L. Goldstein Tyrosine kinase inhibition affects skate anion exchanger isoform I alterations after volume expansion Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2005; 288(4): R885 - R890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Xu, W. Tian, J. N. Lindsley, T. T. Oyama, J. M. Capasso, C. J. Rivard, H. T. Cohen, S. M. Bagnasco, S. Anderson, and D. M. Cohen EphA2: expression in the renal medulla and regulation by hypertonicity and urea stress in vitro and in vivo Am J Physiol Renal Physiol, April 1, 2005; 288(4): F855 - F866. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Cohen SRC family kinases in cell volume regulation Am J Physiol Cell Physiol, March 1, 2005; 288(3): C483 - C493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Musch, D.-L. T. Koomoa, and L. Goldstein Hypotonicity-induced Exocytosis of the Skate Anion Exchanger skAE1: ROLE OF LIPID RAFT REGIONS J. Biol. Chem., September 17, 2004; 279(38): 39447 - 39453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Xu, H. Zhao, W. Tian, K. Yoshida, J.-B. Roullet, and D. M. Cohen Regulation of a Transient Receptor Potential (TRP) Channel by Tyrosine Phosphorylation. SRC FAMILY KINASE-DEPENDENT TYROSINE PHOSPHORYLATION OF TRPV4 ON TYR-253 MEDIATES ITS RESPONSE TO HYPOTONIC STRESS J. Biol. Chem., March 21, 2003; 278(13): 11520 - 11527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Adair-Kirk, F. C. Dorsey, and J. V. Cox Multiple cytoplasmic signals direct the intracellular trafficking of chicken kidney AE1 anion exchangers in MDCK cells J. Cell Sci., February 15, 2003; 116(4): 655 - 663. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Bordin, A. M. Brunati, A. Donella-Deana, B. Baggio, A. Toninello, and G. Clari Band 3 is an anchor protein and a target for SHP-2 tyrosine phosphatase in human erythrocytes Blood, June 17, 2002; 100(1): 276 - 282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Merciris, M.-D. Hardy-Dessources, and F. Giraud Deoxygenation of sickle cells stimulates Syk tyrosine kinase and inhibits a membrane tyrosine phosphatase Blood, November 15, 2001; 98(10): 3121 - 3127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-L. T. Koomoa, M. W. Musch, A. Vaz MacLean, and L. Goldstein Volume-activated trimethylamine oxide efflux in red blood cells of spiny dogfish (Squalus acanthias) Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2001; 281(3): R803 - R810. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Tilbrook, G. A. Palmer, T. Bittorf, D. J. McCarthy, M. J. Wright, M. K. Sarna, D. Linnekin, V. S. Cull, J. H. Williams, E. Ingley, et al. Maturation of Erythroid Cells and Erythroleukemia Development Are Affected by the Kinase Activity of Lyn Cancer Res., March 1, 2001; 61(6): 2453 - 2458. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Yamada, S. Fujieda, S. Yanagi, H. Yamamura, R. Inatome, H. Sunaga, and H. Saito Protein-Tyrosine Kinase Syk Expressed in Human Nasal Fibroblasts and Its Effect on RANTES Production J. Immunol., January 1, 2001; 166(1): 538 - 543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Brunati, L. Bordin, G. Clari, P. James, M. Quadroni, E. Baritono, L. A. Pinna, and A. Donella-Deana Sequential phosphorylation of protein band 3 by Syk and Lyn tyrosine kinases in intact human erythrocytes: identification of primary and secondary phosphorylation sites Blood, August 15, 2000; 96(4): 1550 - 1557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Wittels, E. M. Hubert, M. W. Musch, and L. Goldstein Osmolyte channel regulation by ionic strength in skate RBC Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2000; 279(1): R69 - R76. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mocsai, Z. Jakus, T. Vantus, G. Berton, C. A. Lowell, and E. Ligeti Kinase Pathways in Chemoattractant-Induced Degranulation of Neutrophils: The Role of p38 Mitogen-Activated Protein Kinase Activated by Src Family Kinases J. Immunol., April 15, 2000; 164(8): 4321 - 4331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Adair-Kirk, K. H. Cox, and J. V. Cox Intracellular Trafficking of Variant Chicken Kidney AE1 Anion Exchangers: Role of Alternative NH2 Termini in Polarized Sorting and Golgi Recycling J. Cell Biol., December 13, 1999; 147(6): 1237 - 1248. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
