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J Biol Chem, Vol. 274, Issue 12, 7969-7974, March 19, 1999

Secretagogue-induced Exocytosis Recruits G Protein-gated K⁺ Channels to Plasma Membrane in Endocrine Cells

Ken-ichirou Morishige^§, Atsushi Inanobe, Yukiko Yoshimoto, Hirohisa Kurachi^§, Yuji Murata^§, Yoshimitu Tokunaga^¶, Toshihiro Maeda^¶, Yoshio Maruyama, and Yoshihisa Kurachi

From the Departments of  Pharmacology II and ^§ Obstetrics and Gynecology, Faculty of Medicine and Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, the ^¶ Department of Anatomy I, Shiga University of Medical Science, Shiga 520-2121, and the  Department of Physiology, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980-0872, Japan

Stimulation-regulated fusion of vesicles to the plasma membrane is an essential step for hormone secretion but may also serve for the recruitment of functional proteins to the plasma membrane. While studying the distribution of G protein-gated K⁺ (K_G) channels in the anterior pituitary lobe, we found K_G channel subunits Kir3.1 and Kir3.4 localized on the membranes of intracellular dense core vesicles that contained thyrotropin. Stimulation of these thyrotroph cells with thyrotropin-releasing hormone provoked fusion of vesicles to the plasma membrane, increased expression of Kir3.1 and Kir3.4 subunits in the plasma membrane, and markedly enhanced K_G currents stimulated by dopamine and somatostatin. These data indicate a novel mechanism for the rapid insertion of functional ion channels into the plasma membrane, which could form a new type of negative feedback control loop for hormone secretion in the endocrine system.

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Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

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