HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wu-Wong, J. R. Articles by Fissel, B. Search for Related Content PubMed PubMed Citation Articles by Wu-Wong, J. R. Articles by Fissel, B. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 12, 8103-8110, March 19, 1999

Endothelin Stimulates Glucose Uptake and GLUT4 Translocation via Activation of Endothelin ET_A Receptor in 3T3-L1 Adipocytes

From the Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, Illinois 60064-3500

Endothelin-1 (ET-1) is a 21-amino acid peptide that binds to G-protein-coupled receptors to evoke biological responses. This report studies the effect of ET-1 on regulating glucose transport in 3T3-L1 adipocytes. ET-1, but not angiotensin II, stimulated glucose uptake in a dose-dependent manner with an EC₅₀ value of 0.29 nM and a 2.47-fold stimulation at 100 nM. ET-1 stimulated glucose uptake in differentiated 3T3-L1 cells but had no effect in undifferentiated cells, although ET-1 stimulated phosphatidylinositol hydrolysis to a similar degree in both. The 3T3-L1 cells expressed ~560,000 sites/cell of ET_A receptor, which was not altered during differentiation. Western blot analysis and immunofluorescence staining show that ET-1 stimulated the translocation of insulin-responsive aminopeptidase and GLUT4 to the plasma membrane. The effect of ET-1 on glucose uptake was blocked by A-216546, an antagonist selective for the ET_A receptor. ET-1 treatment did not induce phosphorylation of insulin receptor -subunit, insulin receptor substrate-1, or Akt but stimulated the tyrosyl phosphorylation of a 75-kDa protein. Genistein (100 µM), an inhibitor of tyrosine kinases, inhibited ET-1-stimulated glucose uptake. Our results show that ET-1 stimulates GLUT4 translocation and glucose uptake in 3T3-L1 adipocytes via activation of ET_A receptor.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J. Stockli, J. R. Davey, C. Hohnen-Behrens, A. Xu, D. E. James, and G. Ramm Regulation of Glucose Transporter 4 Translocation by the Rab Guanosine Triphosphatase-Activating Protein AS160/TBC1D4: Role of Phosphorylation and Membrane Association Mol. Endocrinol., December 1, 2008; 22(12): 2703 - 2715. [Abstract] [Full Text] [PDF]

				A. M. McCarthy, K. O. Spisak, J. T. Brozinick, and J. S. Elmendorf Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport Am J Physiol Cell Physiol, November 1, 2006; 291(5): C860 - C868. [Abstract] [Full Text] [PDF]

				N. Wettschureck and S. Offermanns Mammalian G Proteins and Their Cell Type Specific Functions Physiol Rev, October 1, 2005; 85(4): 1159 - 1204. [Abstract] [Full Text] [PDF]

				A.-L. Tondu, C. Robichon, L. Yvan-Charvet, N. Donne, X. Le Liepvre, E. Hajduch, P. Ferre, I. Dugail, and G. Dagher Insulin and Angiotensin II Induce the Translocation of Scavenger Receptor Class B, Type I from Intracellular Sites to the Plasma Membrane of Adipocytes J. Biol. Chem., September 30, 2005; 280(39): 33536 - 33540. [Abstract] [Full Text] [PDF]

				C.-C. Juan, C.-L. Chang, Y.-H. Lai, and L.-T. Ho Endothelin-1 induces lipolysis in 3T3-L1 adipocytes Am J Physiol Endocrinol Metab, June 1, 2005; 288(6): E1146 - E1152. [Abstract] [Full Text] [PDF]

				M. Kanzaki, M. Furukawa, W. Raab, and J. E. Pessin Phosphatidylinositol 4,5-Bisphosphate Regulates Adipocyte Actin Dynamics and GLUT4 Vesicle Recycling J. Biol. Chem., July 16, 2004; 279(29): 30622 - 30633. [Abstract] [Full Text] [PDF]

				P. Liu, B. J. Leffler, L. K. Weeks, G. Chen, C. M. Bouchard, A. B. Strawbridge, and J. S. Elmendorf Sphingomyelinase activates GLUT4 translocation via a cholesterol-dependent mechanism Am J Physiol Cell Physiol, February 1, 2004; 286(2): C317 - C329. [Abstract] [Full Text]

				S. Perrini, A. Natalicchio, L. Laviola, G. Belsanti, C. Montrone, A. Cignarelli, V. Minielli, M. Grano, G. De Pergola, R. Giorgino, et al. Dehydroepiandrosterone Stimulates Glucose Uptake in Human and Murine Adipocytes by Inducing GLUT1 and GLUT4 Translocation to the Plasma Membrane Diabetes, January 1, 2004; 53(1): 41 - 52. [Abstract] [Full Text] [PDF]

				N. Rachdaoui and L. E. Nagy Endothelin-1-stimulated glucose uptake is desensitized by tumor necrosis factor-{alpha} in 3T3-L1 adipocytes Am J Physiol Endocrinol Metab, September 1, 2003; 285(3): E545 - E551. [Abstract] [Full Text] [PDF]

				L.-B. Liu, W. Omata, I. Kojima, and H. Shibata Insulin Recruits GLUT4 from Distinct Compartments via Distinct Traffic Pathways with Differential Microtubule Dependence in Rat Adipocytes J. Biol. Chem., August 8, 2003; 278(32): 30157 - 30169. [Abstract] [Full Text] [PDF]

				J. J. Wilkes, A. Hevener, and J. Olefsky Chronic Endothelin-1 Treatment Leads to Insulin Resistance In Vivo Diabetes, August 1, 2003; 52(8): 1904 - 1909. [Abstract] [Full Text] [PDF]

				N. Rachdaoui, B. M. Sebastian, and L. E. Nagy Chronic ethanol feeding impairs endothelin-1-stimulated glucose uptake via decreased G{alpha}11 expression in rat adipocytes Am J Physiol Endocrinol Metab, August 1, 2003; 285(2): E303 - E310. [Abstract] [Full Text] [PDF]

				S. Mora, P. L. Durham, J. R. Smith, A. F. Russo, A. Jeromin, and J. E. Pessin NCS-1 Inhibits Insulin-stimulated GLUT4 Translocation in 3T3L1 Adipocytes through a Phosphatidylinositol 4-Kinase-dependent Pathway J. Biol. Chem., July 19, 2002; 277(30): 27494 - 27500. [Abstract] [Full Text] [PDF]

				S. R. Keller, A. C. Davis, and K. B. Clairmont Mice Deficient in the Insulin-regulated Membrane Aminopeptidase Show Substantial Decreases in Glucose Transporter GLUT4 Levels but Maintain Normal Glucose Homeostasis J. Biol. Chem., May 10, 2002; 277(20): 17677 - 17686. [Abstract] [Full Text] [PDF]

				S. F. Kralik, P. Liu, B. J. Leffler, and J. S. Elmendorf Ceramide and Glucosamine Antagonism of Alternate Signaling Pathways Regulating Insulin- and Osmotic Shock-Induced Glucose Transporter 4 Translocation Endocrinology, January 1, 2002; 143(1): 37 - 46. [Abstract] [Full Text] [PDF]

				J. G. Park, A. Bose, J. Leszyk, and M. P. Czech PYK2 as a Mediator of Endothelin-1/Galpha 11 Signaling to GLUT4 Glucose Transporters J. Biol. Chem., December 14, 2001; 276(51): 47751 - 47754. [Abstract] [Full Text] [PDF]

				T. Imamura, J. Huang, S. Dalle, S. Ugi, I. Usui, L. M. Luttrell, W. E. Miller, R. J. Lefkowitz, and J. M. Olefsky beta -Arrestin-mediated Recruitment of the Src Family Kinase Yes Mediates Endothelin-1-stimulated Glucose Transport J. Biol. Chem., November 16, 2001; 276(47): 43663 - 43667. [Abstract] [Full Text] [PDF]

				M. D. Apostolova, S. Chen, S. Chakrabarti, and M. G. Cherian High-glucose-induced metallothionein expression in endothelial cells: an endothelin-mediated mechanism Am J Physiol Cell Physiol, September 1, 2001; 281(3): C899 - C907. [Abstract] [Full Text] [PDF]

				A. Bose, A. D. Cherniack, S. E. Langille, S. M. C. Nicoloro, J. M. Buxton, J. G. Park, A. Chawla, and M. P. Czech G{alpha}11 Signaling through ARF6 Regulates F-Actin Mobilization and GLUT4 Glucose Transporter Translocation to the Plasma Membrane Mol. Cell. Biol., August 1, 2001; 21(15): 5262 - 5275. [Abstract] [Full Text] [PDF]

				J. T. R. Lawrence and M. J. Birnbaum ADP-Ribosylation Factor 6 Delineates Separate Pathways Used by Endothelin 1 and Insulin for Stimulating Glucose Uptake in 3T3-L1 Adipocytes Mol. Cell. Biol., August 1, 2001; 21(15): 5276 - 5285. [Abstract] [Full Text] [PDF]

				K.-i. Ishibashi, T. Imamura, P. M. Sharma, S. Ugi, and J. M. Olefsky The Acute and Chronic Stimulatory Effects of Endothelin-1 on Glucose Transport Are Mediated by Distinct Pathways in 3T3-L1 Adipocytes Endocrinology, December 1, 2000; 141(12): 4623 - 4628. [Abstract] [Full Text] [PDF]

				G. Müller, S. Wied, and W. Frick Cross Talk of pp125FAK and pp59Lyn Non-Receptor Tyrosine Kinases to Insulin-Mimetic Signaling in Adipocytes Mol. Cell. Biol., July 1, 2000; 20(13): 4708 - 4723. [Abstract] [Full Text]

				J. R. Wu-Wong, W. J. Chiou, and J. Wang Extracellular Signal-Regulated Kinases Are Involved in the Antiapoptotic Effect of Endothelin-1 J. Pharmacol. Exp. Ther., May 1, 2000; 293(2): 514 - 521. [Abstract] [Full Text]

				M. Kawanishi, Y. Tamori, H. Okazawa, S. Araki, H. Shinoda, and M. Kasuga Role of SNAP23 in Insulin-induced Translocation of GLUT4 in 3T3-L1 Adipocytes. MEDIATION OF COMPLEX FORMATION BETWEEN SYNTAXIN4 AND VAMP2 J. Biol. Chem., March 10, 2000; 275(11): 8240 - 8247. [Abstract] [Full Text] [PDF]

				T. Imamura, K.-i. Ishibashi, S. Dalle, S. Ugi, and J. M. Olefsky Endothelin-1-induced GLUT4 Translocation Is Mediated via Galpha q/11 Protein and Phosphatidylinositol 3-Kinase in 3T3-L1 Adipocytes J. Biol. Chem., November 19, 1999; 274(47): 33691 - 33695. [Abstract] [Full Text] [PDF]

				L. F. Cotlin, M. A. Siddiqui, F. Simpson, and J. F. Collawn Casein Kinase II Activity Is Required for Transferrin Receptor Endocytosis J. Biol. Chem., October 22, 1999; 274(43): 30550 - 30556. [Abstract] [Full Text] [PDF]

				H.-J. Lee, M. Chun, and K. V. Kandror Tip60 and HDAC7 Interact with the Endothelin Receptor A and May Be Involved in Downstream Signaling J. Biol. Chem., May 11, 2001; 276(20): 16597 - 16600. [Abstract] [Full Text] [PDF]

				A. Janez, D. S. Worrall, T. Imamura, P. M. Sharma, and J. M. Olefsky The Osmotic Shock-induced Glucose Transport Pathway in 3T3-L1 Adipocytes Is Mediated by Gab-1 and Requires Gab-1-associated Phosphatidylinositol 3-Kinase Activity for Full Activation J. Biol. Chem., August 25, 2000; 275(35): 26870 - 26876. [Abstract] [Full Text] [PDF]

				Y. Xiong, H. Tanaka, J. A. Richardson, S. C. Williams, C. A. Slaughter, M. Nakamura, J.-L. Chen, and M. Yanagisawa Endothelin-1 Stimulates Leptin Production in Adipocytes J. Biol. Chem., July 20, 2001; 276(30): 28471 - 28477. [Abstract] [Full Text] [PDF]