HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Li, X.-F. Articles by Lytton, J. Search for Related Content PubMed PubMed Citation Articles by Li, X.-F. Articles by Lytton, J. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 12, 8153-8160, March 19, 1999

A Circularized Sodium-Calcium Exchanger Exon 2 Transcript

From the Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta T2N 4N1, Canada

Previous reports of Na/Ca exchanger gene 1 (NCX1) expression have revealed a major RNA transcript of 7 kilobase pairs (kb), minor transcripts of ~13 and ~4 kb, and a relatively abundant 1.8-kb RNA band. In the present report we demonstrate that the 1.8-kb message, which has a tissue and subcellular distribution matching that of full-length NCX1 but is not polyadenylated, corresponds to a perfectly circularized exon 2 species. The circular transcript contained the normal NCX1 start codon, a new stop codon introduced as a consequence of circularization, and encoded a protein corresponding to the NH₂-terminal portion of NCX1, terminating just after amino acid 600 in the cytoplasmic loop. A linear version of the circular transcript was prepared and transfected into HEK-293 cells. A protein, matching the predicted size of ~70 kDa, was expressed, and the transfected cells possessed Na/Ca exchange activity. Although in native tissue we could not detect a protein corresponding exactly to that predicted from the circular transcript, a prominent band of slightly shorter size, possibly representing further proteolytic processing of circular transcript protein, was observed in membranes from LLC-MK2 cells and rat kidney.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J.-P. Li, H. Kajiya, F. Okamoto, A. Nakao, T. Iwamoto, and K. Okabe Three Na+/Ca2+ Exchanger (NCX) Variants Are Expressed in Mouse Osteoclasts and Mediate Calcium Transport during Bone Resorption Endocrinology, May 1, 2007; 148(5): 2116 - 2125. [Abstract] [Full Text] [PDF]

				H. Suzuki, Y. Zuo, J. Wang, M. Q. Zhang, A. Malhotra, and A. Mayeda Characterization of RNase R-digested cellular RNA source that consists of lariat and circular RNAs from pre-mRNA splicing. Nucleic Acids Res., January 1, 2006; 34(8): e63 - e63. [Abstract] [Full Text] [PDF]

				E Renkonen, H Lohi, H J Jarvinen, J-P Mecklin, and P Peltomaki Novel splicing associations of hereditary colon cancer related DNA mismatch repair gene mutations J. Med. Genet., July 1, 2004; 41(7): e95 - e95. [Full Text] [PDF]

				X. Cai and J. Lytton Molecular Cloning of a Sixth Member of the K+-dependent Na+/Ca2+ Exchanger Gene Family, NCKX6 J. Biol. Chem., February 13, 2004; 279(7): 5867 - 5876. [Abstract] [Full Text] [PDF]

				F Gualandi, C Trabanelli, P Rimessi, E Calzolari, L Toffolatti, T Patarnello, G Kunz, F Muntoni, and A Ferlini Multiple exon skipping and RNA circularisation contribute to the severe phenotypic expression of exon 5 dystrophin deletion J. Med. Genet., August 1, 2003; 40(8): e100 - 100. [Full Text] [PDF]

				J. G. Muller, Y. Isomatsu, S. V. Koushik, M. O'Quinn, L. Xu, C. S. Kappler, E. Hapke, M. R. Zile, S. J. Conway, and D. R. Menick Cardiac-Specific Expression and Hypertrophic Upregulation of the Feline Na+-Ca2+ Exchanger Gene H1-Promoter in a Transgenic Mouse Model Circ. Res., February 8, 2002; 90(2): 158 - 164. [Abstract] [Full Text] [PDF]

				S. C. Warner, C. Finta, and P. G. Zaphiropoulos Intergenic Transcripts Containing a Novel Human Cytochrome P450 2C Exon 1 Spliced to Sequences from the CYP2C9 Gene Mol. Biol. Evol., October 1, 2001; 18(10): 1841 - 1848. [Abstract] [Full Text] [PDF]

				B. Fraysse, T. Rouaud, M. Millour, J. Fontaine-Perus, M.-F. Gardahaut, and D. O. Levitsky Expression of the Na+/Ca2+ exchanger in skeletal muscle Am J Physiol Cell Physiol, January 1, 2001; 280(1): C146 - C154. [Abstract] [Full Text] [PDF]

				R. T. Szerencsei, J. E. Tucker, C. B. Cooper, R. J. Winkfein, P. J. Farrell, K. Iatrou, and P. P. M. Schnetkamp Minimal Domain Requirement for Cation Transport by the Potassium-dependent Na/Ca-K Exchanger. COMPARISON WITH AN NCKX PARALOG FROM CAENORHABDITIS ELEGANS J. Biol. Chem., January 7, 2000; 275(1): 669 - 676. [Abstract] [Full Text] [PDF]

				J. Kasir, X. Ren, I. Furman, and H. Rahamimoff Truncation of the C Terminus of the Rat Brain Na+-Ca2+ Exchanger RBE-1 (NCX1.4) Impairs Surface Expression of the Protein J. Biol. Chem., August 27, 1999; 274(35): 24873 - 24880. [Abstract] [Full Text] [PDF]

				M. Ottolia, S. John, Z. Qiu, and K. D. Philipson Split Na+-Ca2+ Exchangers. IMPLICATIONS FOR FUNCTION AND EXPRESSION J. Biol. Chem., May 25, 2001; 276(22): 19603 - 19609. [Abstract] [Full Text] [PDF]

				A. Kraev, B. D. Quednau, S. Leach, X.-F. Li, H. Dong, R. Winkfein, M. Perizzolo, X. Cai, R. Yang, K. D. Philipson, et al. Molecular Cloning of a Third Member of the Potassium-dependent Sodium-Calcium Exchanger Gene Family, NCKX3 J. Biol. Chem., June 15, 2001; 276(25): 23161 - 23172. [Abstract] [Full Text] [PDF]

				J. G. Muller, Y. Isomatsu, S. V. Koushik, M. O'Quinn, L. Xu, C. S. Kappler, E. Hapke, M. R. Zile, S. J. Conway, and D. R. Menick Cardiac-Specific Expression and Hypertrophic Upregulation of the Feline Na+-Ca2+ Exchanger Gene H1-Promoter in a Transgenic Mouse Model Circ. Res., February 8, 2002; 90(2): 158 - 164. [Abstract] [Full Text] [PDF]