|
J Biol Chem, Vol. 274, Issue 12, 8261-8268, March 19, 1999
Reduced Capacitative Calcium Entry Correlates with Vesicle
Accumulation and Apoptosis
Supriya
Jayadev,
John G.
Petranka,
Sendhil K.
Cheran,
Jennifer
A.
Biermann,
J. Carl
Barrett, and
Elizabeth
Murphy
From the Laboratory of Molecular Carcinogenesis, National Institute
of Environmental Health Sciences,
Research Triangle Park, North Carolina 27709
A preneoplastic variant of Syrian hamster
embryo cells, sup(+), exhibits decreased endoplasmic reticulum calcium
levels and subsequently undergoes apoptosis in low serum conditions
(Preston, G. A., Barrett, J. C., Biermann, J. A., and
Murphy, E. (1997) Cancer Res. 57, 537-542). This decrease
in endoplasmic reticulum calcium appears to be due, at least in part,
to reduced capacitative calcium entry at the plasma membrane. Thus we
investigated whether inhibition of capacitative calcium entry per
se could reduce endoplasmic reticulum calcium and induce
apoptosis of cells. We find that treatment with either SKF96365
(30-100 µM) or cell-impermeant 1,2-bis(o-amino-5-bromophenoxy)ethane-N,N,N',N'-tetraacetic
acid (5-10 mM) is able to induce apoptosis of
cells in conditions where apoptosis does not normally occur. Because
previous work has implicated vesicular trafficking as a mechanism of
regulating capacitative calcium entry, we investigated whether
disruption of vesicular trafficking could lead to decreased
capacitative calcium entry and subsequent apoptosis of cells.
Coincident with low serum-induced apoptosis, we observed an
accumulation of vesicles within the cell, suggesting deregulated
vesicle trafficking. Treatment of cells with bafilomycin (30-100
nM), an inhibitor of the endosomal proton ATPase, produced
an accumulation of vesicles, decreased capacitative entry, and induced
apoptosis. These data suggest that deregulation of vesicular transport
results in reduced capacitative calcium entry which in turn results in apoptosis.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
S.-Y. Lee, B.-H. Choi, E.-M. Hur, J.-H. Lee, S.-J. Lee, C. O. Lee, and K.-T. Kim
Norepinephrine activates store-operated Ca2+ entry coupled to large-conductance Ca2+-activated K+ channels in rat pinealocytes
Am J Physiol Cell Physiol,
April 1, 2006;
290(4):
C1060 - C1066.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Tong, H. A. Rockman, W. J. Koch, C. Steenbergen, and E. Murphy
G Protein-Coupled Receptor Internalization Signaling Is Required for Cardioprotection in Ischemic Preconditioning
Circ. Res.,
April 30, 2004;
94(8):
1133 - 1141.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Levine, B. Belenghi, H. Damari-Weisler, and D. Granot
Vesicle-associated Membrane Protein of Arabidopsis Suppresses Bax-induced Apoptosis in Yeast Downstream of Oxidative Burst
J. Biol. Chem.,
November 30, 2001;
276(49):
46284 - 46289.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. M. Sheridan, T. Force, H.-J. Yoon, E. O'Leary, G. Choukroun, M. R. Taheri, and J. V. Bonventre
PLIP, a Novel Splice Variant of Tip60, Interacts with Group IV Cytosolic Phospholipase A2, Induces Apoptosis, and Potentiates Prostaglandin Production
Mol. Cell. Biol.,
July 15, 2001;
21(14):
4470 - 4481.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Jayadev, J. C. Barrett, and E. Murphy
Elevated ceramide is downstream of altered calcium homeostasis in low serum-induced apoptosis
Am J Physiol Cell Physiol,
November 1, 2000;
279(5):
C1640 - C1647.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. S. Williams, J. N. French, M. Gilbert, A. A. Rangaswami, J. Walleczek, and S. J. Knox
Bcl-2 Overexpression Results in Enhanced Capacitative Calcium Entry and Resistance to SKF-96395-induced Apoptosis
Cancer Res.,
August 1, 2000;
60(16):
4358 - 4361.
[Abstract]
[Full Text]
|
 |
|

|
 |

|
 |
 
L. Zhu, S. Ling, X.-D. Yu, L. K. Venkatesh, T. Subramanian, G. Chinnadurai, and T. H. Kuo
Modulation of Mitochondrial Ca2+ Homeostasis by Bcl-2
J. Biol. Chem.,
November 19, 1999;
274(47):
33267 - 33273.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. Kunzelmann-Marche, J.-M. Freyssinet, and M. C. Martinez
Regulation of Phosphatidylserine Transbilayer Redistribution by Store-operated Ca2+ Entry. ROLE OF ACTIN CYTOSKELETON
J. Biol. Chem.,
February 9, 2001;
276(7):
5134 - 5139.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Petranka, G. Wright, R. A. Forbes, and E. Murphy
Elevated Calcium in Preneoplastic Cells Activates NF-kappa B and Confers Resistance to Apoptosis
J. Biol. Chem.,
September 28, 2001;
276(40):
37102 - 37108.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|