HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lareyre, J.-J. Articles by Matusik, R. J. Search for Related Content PubMed PubMed Citation Articles by Lareyre, J.-J. Articles by Matusik, R. J. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 12, 8282-8290, March 19, 1999

A 5-Kilobase Pair Promoter Fragment of the Murine Epididymal Retinoic Acid-binding Protein Gene Drives the Tissue-specific, Cell-specific, and Androgen-regulated Expression of a Foreign Gene in the Epididymis of Transgenic Mice

Jean-Jacques Lareyre, Tania Z. Thomas^¶, Wen-Li Zheng, Susan Kasper^¶**, David E. Ong, Marie-Claire Orgebin-Crist^**, and Robert J. Matusik^¶**

From the Departments of  Obstetrics and Gynecology, ^¶ Urologic Surgery,  Biochemistry, and ^** Cell Biology, and the  Center for Reproductive Biology Research, Vanderbilt University, School of Medicine, Nashville, Tennessee 37232-2633

The murine epididymis synthesizes and secretes a retinoic acid-binding protein (mE-RABP) that belongs to the lipocalin superfamily. The gene encoding mE-RABP is specifically expressed in the mouse mid/distal caput epididymidis under androgen control. In transgenic mice, a 5-kilobase pair (kb) promoter fragment, but not a 0.6-kb fragment, of the mE-RABP gene driving the chloramphenicol acetyltransferase (CAT) reporter gene restricted high level of transgene expression to the caput epididymidis. No transgene expression was detected in any other male or female tissues. Immunolocalization of the CAT protein and in situ hybridization of the corresponding CAT mRNA indicated that transgene expression occurred in the principal cells of the mid/distal caput epididymidis, thereby mimicking the spatial endogenous mE-RABP gene expression. Transgene and mE-RABP gene expression was detected from 30 days and progressively increased until 60 days of age. Castration, efferent duct ligation, and hormone replacement studies demonstrated that transgene expression was specifically regulated by androgen but not by any other testicular factors. Altogether, our results demonstrate that the 5-kb promoter fragment of the mE-RABP gene contains all of the information required for the hormonal regulation and the spatial and temporal expression of the mE-RABP gene in the epididymis.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				S. A. Jelinsky, T. T. Turner, H. J. Bang, J. N. Finger, M. K. Solarz, E. Wilson, E. L. Brown, G. S. Kopf, and D. S. Johnston The Rat Epididymal Transcriptome: Comparison of Segmental Gene Expression in the Rat and Mouse Epididymides Biol Reprod, April 1, 2007; 76(4): 561 - 570. [Abstract] [Full Text] [PDF]

				X. Yu, K. Suzuki, Y. Wang, A. Gupta, R. Jin, M.-C. Orgebin-Crist, and R. Matusik The Role of Forkhead Box A2 to Restrict Androgen-Regulated Gene Expression of Lipocalin 5 in the Mouse Epididymis Mol. Endocrinol., October 1, 2006; 20(10): 2418 - 2431. [Abstract] [Full Text] [PDF]

				J. L. Tomsig, S. Usanovic, and T. T. Turner Growth Factor-Stimulated Mitogen-Activated Kinase (MAPK) Phosphorylation in the Rat Epididymis Is Limited by Segmental Boundaries Biol Reprod, October 1, 2006; 75(4): 598 - 604. [Abstract] [Full Text] [PDF]

				N. Hsia, J. P. Brousal, S. R. Hann, and G. A. Cornwall Recapitulation of Germ Cell- and Pituitary-Specific Expression With 1.6 kb of the Cystatin-Related Epididymal Spermatogenic (Cres) Gene Promoter in Transgenic Mice J Androl, March 1, 2005; 26(2): 249 - 257. [Abstract] [Full Text] [PDF]

				K. Suzuki, Y. Araki, M.-Y. Zhu, J.-J. Lareyre, R. J. Matusik, and M.-C. Orgebin-Crist The 5'-Flanking Region of the Murine Epididymal Protein of 17 Kilodaltons Gene Targets Transgene Expression in the Epididymis Endocrinology, March 1, 2003; 144(3): 877 - 886. [Abstract] [Full Text] [PDF]

				S. Fouchecourt, J.-J. Lareyre, P. Chaurand, B. B. DaGue, K. Suzuki, D. E. Ong, G. E. Olson, R. J. Matusik, R. M. Caprioli, and M.-C. Orgebin-Crist Identification, Immunolocalization, Regulation, and Postnatal Development of the Lipocalin EP17 (Epididymal Protein of 17 Kilodaltons) in the Mouse and Rat Epididymis Endocrinology, March 1, 2003; 144(3): 887 - 900. [Abstract] [Full Text] [PDF]

				S. Fouchecourt, S. Castella, F. Dacheux, and J.-L. Dacheux Prostaglandin D2 Synthase Secreted in the Caput Epididymidis Displays Spatial and Temporal Delay Between Messenger RNA and Protein Expression During Postnatal Development Biol Reprod, January 1, 2003; 68(1): 174 - 179. [Abstract] [Full Text] [PDF]

				M. K. Rao, C. M. Wayne, and M. F. Wilkinson Pem Homeobox Gene Regulatory Sequences That Direct Androgen-dependent Developmentally Regulated Gene Expression in Different Subregions of the Epididymis J. Biol. Chem., December 6, 2002; 277(50): 48771 - 48778. [Abstract] [Full Text] [PDF]

				Y. Araki, K. Suzuki, R. J. Matusik, M. Obinata, and M.-C. Orgebin-Crist Immortalized Epididymal Cell Lines From Transgenic Mice Overexpressing Temperature-Sensitive Simian Virus 40 Large T-Antigen Gene J Androl, November 1, 2002; 23(6): 854 - 869. [Abstract] [Full Text] [PDF]

				P. Sipila, T. G. Cooper, C.-H. Yeung, M. Mustonen, J. Penttinen, J. Drevet, I. Huhtaniemi, and M. Poutanen Epididymal Dysfunction Initiated by the Expression of Simian Virus 40 T-Antigen Leads to Angulated Sperm Flagella and Infertility in Transgenic Mice Mol. Endocrinol., November 1, 2002; 16(11): 2603 - 2617. [Abstract] [Full Text] [PDF]

				P. Val, A. Martinez, I. Sahut-Barnola, C. Jean, G. Veyssiere, and A.-M. Lefrancois-Martinez A 77-Base Pair LINE-Like Sequence Elicits Androgen-Dependent mvdp/akr1-b7 Expression in Mouse Vas Deferens, But Is Dispensable for Adrenal Expression in Rats Endocrinology, September 1, 2002; 143(9): 3435 - 3448. [Abstract] [Full Text] [PDF]

				P. P. Lahti, R. Shariatmadari, J. K. Penttinen, J. R. Drevet, B. Haendler, M. Vierula, M. Parvinen, I. T. Huhtaniemi, and M. Poutanen Evaluation of the 5'-Flanking Regions of Murine Glutathione Peroxidase Five and Cysteine-Rich Secretory Protein-1 Genes for Directing Transgene Expression in Mouse Epididymis Biol Reprod, April 1, 2001; 64(4): 1115 - 1121. [Abstract] [Full Text]

				J.-J. Lareyre, V. P. Winfrey, S. Kasper, D. E. Ong, R. J. Matusik, G. E. Olson, and M.-C. Orgebin-Crist Gene Duplication Gives Rise to a New 17-Kilodalton Lipocalin That Shows Epididymal Region-Specific Expression and Testicular Factor(s) Regulation Endocrinology, March 1, 2001; 142(3): 1296 - 1308. [Abstract] [Full Text] [PDF]

				J. Zhang, T. Z. Thomas, S. Kasper, and R. J. Matusik A Small Composite Probasin Promoter Confers High Levels of Prostate-Specific Gene Expression through Regulation by Androgens and Glucocorticoids in Vitro and in Vivo Endocrinology, December 1, 2000; 141(12): 4698 - 4710. [Abstract] [Full Text] [PDF]

				J.-J. Lareyre, K. Reid, C. Nelson, S. Kasper, P. S. Rennie, M.-C. Orgebin-Crist, and R. J. Matusik Characterization of an Androgen-Specific Response Region Within the 5' Flanking Region of the Murine Epididymal Retinoic Acid Binding Protein Gene Biol Reprod, December 1, 2000; 63(6): 1881 - 1892. [Abstract] [Full Text]