JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamauchi, K.
Right arrow Articles by Tata, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamauchi, K.
Right arrow Articles by Tata, J. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 274, Issue 13, 8460-8469, March 26, 1999

Xenopus Cytosolic Thyroid Hormone-binding Protein (xCTBP) Is Aldehyde Dehydrogenase Catalyzing the Formation of Retinoic Acid

Kiyoshi YamauchiDagger , Jun-ichiro NakajimaDagger , Hiroaki Hayashi, Ryuya Horiuchiparallel , and Jamshed R. Tata**

From the Dagger  Department of Biology, Faculty of Science, Shizuoka University, Shizuoka 422-8529, the  Gunma Prefectural College of Health Sciences, Maebashi 371-0052, the parallel  Department of Pharmacy, Gunma University School of Medicine, Maebashi 371-8511, Japan, and the ** Laboratory of Developmental Biochemistry, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom

Amino acid sequencing of an internal peptide fragment derived from purified Xenopus cytosolic thyroid hormone-binding protein (xCTBP) demonstrates high similarity to the corresponding sequence of mammalian aldehyde dehydrogenase 1 (ALDH1) (Yamauchi, K., and Tata, J. R. (1994) Eur. J. Biochem. 225, 1105-1112). Here we show that xCTBP was co-purified with ALDH and 3,3',5-triiodo-L-thyronine (T3) binding activities. By photoaffinity labeling with [125I]T3, a T3-binding site in the xCTBP was estimated to reside in amino acid residues 93-114, which is distinct from the active site of the enzyme but present in the NAD+ binding domain. The amino acid sequences deduced from the two isolated xALDH1 cDNAs (xALDH1-I and xALDH1-II) were 94.6% identical to each other and very similar to those of mammalian ALDH1 enzymes. The two recombinant xALDH1 proteins exhibit both T3 binding activity and ALDH activity converting retinal to retinoic acid (RA), which are similar to those of xCTBP. The mRNAs were present abundantly in kidney and intestine of adult female Xenopus. Interestingly, their T3 binding activities were inhibited by NAD+ and NADH but not by NADP+ and NADPH, whereas NAD+ was required for their ALDH activities. Our results demonstrate that xCTBP is identical to ALDH1 and suggest that this protein might modulate RA synthesis and intracellular level of free T3.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
S. Suzuki, N. Suzuki, J.-i. Mori, A. Oshima, S. Usami, and K. Hashizume
{micro}-Crystallin as an Intracellular 3,5,3'-Triiodothyronine Holder in Vivo
Mol. Endocrinol., April 1, 2007; 21(4): 885 - 894.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Pappa, D. Brown, Y. Koutalos, J. DeGregori, C. White, and V. Vasiliou
Human Aldehyde Dehydrogenase 3A1 Inhibits Proliferation and Promotes Survival of Human Corneal Epithelial Cells
J. Biol. Chem., July 29, 2005; 280(30): 27998 - 28006.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
X. Fan, A. Molotkov, S.-I. Manabe, C. M. Donmoyer, L. Deltour, M. H. Foglio, A. E. Cuenca, W. S. Blaner, S. A. Lipton, and G. Duester
Targeted Disruption of Aldh1a1 (Raldh1) Provides Evidence for a Complex Mechanism of Retinoic Acid Synthesis in the Developing Retina
Mol. Cell. Biol., July 1, 2003; 23(13): 4637 - 4648.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Moreno, E. Silvestri, A. Lombardi, T. J. Visser, F. Goglia, and A. Lanni
Identification of 3,5-Diiodo-L-Thyronine-Binding Proteins in Rat Liver Cytosol by Photoaffinity Labeling
Endocrinology, June 1, 2003; 144(6): 2297 - 2303.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Piatigorsky, Z. Kozmik, J. Horwitz, L. Ding, E. Carosa, W. G. Robison Jr., P. J. Steinbach, and E. R. Tamm
Omega -Crystallin of the Scallop Lens. A DIMERIC ALDEHYDE DEHYDROGENASE CLASS 1/2 ENZYME-CRYSTALLIN
J. Biol. Chem., December 22, 2000; 275(52): 41064 - 41073.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.