JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by George, C. H.
Right arrow Articles by Evans, W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by George, C. H.
Right arrow Articles by Evans, W. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 274, Issue 13, 8678-8685, March 26, 1999

Intracellular Trafficking Pathways in the Assembly of Connexins into Gap Junctions

Christopher H. George, Jonathan M. Kendall, and W. Howard Evans

From the Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff CF4 4XN, Wales, United Kingdom

Trafficking pathways underlying the assembly of connexins into gap junctions were examined using living COS-7 cells expressing a range of connexin-aequorin (Cx-Aeq) chimeras. By measuring the chemiluminescence of the aequorin fusion partner, the translocation of oligomerized connexins from intracellular stores to the plasma membrane was shown to occur at different rates that depended on the connexin isoform. Treatment of COS-7 cells expressing Cx32-Aeq and Cx43-Aeq with brefeldin A inhibited the movement of these chimera to the plasma membrane by 84 ± 4 and 88 ± 4%, respectively. Nocodazole treatment of the cells expressing Cx32-Aeq and Cx43-Aeq produced 29 ± 16 and 4 ± 7% inhibition, respectively. In contrast, the transport of Cx26 to the plasma membrane, studied using a construct (Cx26/43T-Aeq) in which the short cytoplasmic carboxyl-terminal tail of Cx26 was replaced with the extended carboxyl terminus of Cx43, was inhibited 89 ± 5% by nocodazole and was minimally affected by exposure of cells to brefeldin A (17 ±11%). The transfer of Lucifer yellow across gap junctions between cells expressing wild-type Cx32, Cx43, and the corresponding Cx32-Aeq and Cx43-Aeq chimeras was reduced by nocodazole treatment and abolished by brefeldin A treatment. However, the extent of dye coupling between cells expressing wild-type Cx26 or the Cx26/43T-Aeq chimeras was not significantly affected by brefeldin A treatment, but after nocodazole treatment, transfer of dye to neighboring cells was greatly reduced. These contrasting effects of brefeldin A and nocodazole on the trafficking properties and intercellular dye transfer are interpreted to suggest that two pathways contribute to the routing of connexins to the gap junction.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
T. Thomas, K. Jordan, J. Simek, Q. Shao, C. Jedeszko, P. Walton, and D. W. Laird
Mechanisms of Cx43 and Cx26 transport to the plasma membrane and gap junction regeneration
J. Cell Sci., October 1, 2005; 118(19): 4451 - 4462.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Maza, J. D. Sarma, and M. Koval
Defining a Minimal Motif Required to Prevent Connexin Oligomerization in the Endoplasmic Reticulum
J. Biol. Chem., June 3, 2005; 280(22): 21115 - 21121.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. Fiorini, B. Mograbi, L. Cronier, I. Bourget, X. Decrouy, M. Nebout, B. Ferrua, A. Malassine, M. Samson, P. Fenichel, et al.
Dominant negative effect of connexin33 on gap junctional communication is mediated by connexin43 sequestration
J. Cell Sci., September 15, 2004; 117(20): 4665 - 4672.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Gemel, V. Valiunas, P. R. Brink, and E. C. Beyer
Connexin43 and connexin26 form gap junctions, but not heteromeric channels in co-expressing cells
J. Cell Sci., May 15, 2004; 117(12): 2469 - 2480.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
P. E.M. Martin and W.H. Evans
Incorporation of connexins into plasma membranes and gap junctions
Cardiovasc Res, May 1, 2004; 62(2): 378 - 387.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Guo, C. Martinez-Williams, and D. E. Rannels
Gap junction-microtubule associations in rat alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1213 - L1221.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. C. SAEZ, V. M. BERTHOUD, M. C. BRANES, A. D. MARTINEZ, and E. C. BEYER
Plasma Membrane Channels Formed by Connexins: Their Regulation and Functions
Physiol Rev, October 1, 2003; 83(4): 1359 - 1400.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
T. Husoy, V. Cruciani, H. K. Knutsen, S.-O. Mikalsen, H. B. Olstorn, and J. Alexander
Cells heterozygous for the ApcMin mutation have decreased gap junctional intercellular communication and connexin43 level, and reduced microtubule polymerization
Carcinogenesis, April 1, 2003; 24(4): 643 - 650.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Oshima, T. Doi, K. Mitsuoka, S. Maeda, and Y. Fujiyoshi
Roles of Met-34, Cys-64, and Arg-75 in the Assembly of Human Connexin 26. IMPLICATION FOR KEY AMINO ACID RESIDUES FOR CHANNEL FORMATION AND FUNCTION
J. Biol. Chem., January 10, 2003; 278(3): 1807 - 1816.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Tu, T.-T. Sun, and G. Kreibich
Specific Heterodimer Formation Is a Prerequisite for Uroplakins to Exit from the Endoplasmic Reticulum
Mol. Biol. Cell, December 1, 2002; 13(12): 4221 - 4230.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. D. Sarma, F. Wang, and M. Koval
Targeted Gap Junction Protein Constructs Reveal Connexin-specific Differences in Oligomerization
J. Biol. Chem., May 31, 2002; 277(23): 20911 - 20918.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Das Sarma, R. A. Meyer, F. Wang, V. Abraham, C. W. Lo, and M. Koval
Multimeric connexin interactions prior to the trans-Golgi network
J. Cell Sci., March 13, 2002; 114(22): 4013 - 4024.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. E. M. Martin, G. Blundell, S. Ahmad, R. J. Errington, and W. H. Evans
Multiple pathways in the trafficking and assembly of connexin 26, 32 and 43 into gap junction intercellular communication channels
J. Cell Sci., January 11, 2001; 114(21): 3845 - 3855.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Boitano and W. H. Evans
Connexin mimetic peptides reversibly inhibit Ca2+ signaling through gap junctions in airway cells
Am J Physiol Lung Cell Mol Physiol, October 1, 2000; 279(4): L623 - L630.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Calvo, A. Pol, A. Lu, D. Ortega, M. Pons, J. Blasi, and C. Enrich
Cellubrevin Is Present in the Basolateral Endocytic Compartment of Hepatocytes and Follows the Transcytotic Pathway after IgA Internalization
J. Biol. Chem., March 10, 2000; 275(11): 7910 - 7917.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Falk
Connexin-specific distribution within gap junctions revealed in living cells
J. Cell Sci., January 11, 2000; 113(22): 4109 - 4120.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
A. Paulson, P. Lampe, R. Meyer, E TenBroek, M. Atkinson, T. Walseth, and R. Johnson
Cyclic AMP and LDL trigger a rapid enhancement in gap junction assembly through a stimulation of connexin trafficking
J. Cell Sci., January 9, 2000; 113(17): 3037 - 3049.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.