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J Biol Chem, Vol. 274, Issue 14, 9183-9192, April 2, 1999
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From the Metallothionein (MT) is a small cysteine-rich
protein thought to play a critical role in cellular detoxification of
inorganic species by sequestering metal ions that are present in
elevated concentrations. We demonstrate here that metallothionein can
play an important role at the other end of the homeostatic spectrum by
scavenging an essential metal in a mouse fibroblast cell line that has
been cultured under conditions of extreme zinc deprivation (LZA-LTK Department of Biochemistry,
). These cells unexpectedly produce
constitutively high levels of metallothionein mRNA; however, the MT
protein accumulates only when high concentrations of zinc are provided
in the media. Until this MT pool is saturated, no measurable zinc
remains in the external media. In this case, zinc deprivation leads to
amplification of the MT gene locus in the LZA-LTK cell
line. Furthermore, the intracellular zinc levels in the fully adapted
cells remain at the normal level of 0.4 fmol zinc/cell, even when
extracellular zinc concentration is decreased by 2 orders of magnitude
relative to normal media.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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