HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gurezka, R. Articles by Langosch, D. Search for Related Content PubMed PubMed Citation Articles by Gurezka, R. Articles by Langosch, D. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 14, 9265-9270, April 2, 1999

A Heptad Motif of Leucine Residues Found in Membrane Proteins Can Drive Self-assembly of Artificial Transmembrane Segments

From the Universität Heidelberg, Neurobiology Department, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany

Specific interactions between -helical transmembrane segments are important for folding and/or oligomerization of membrane proteins. Previously, we have shown that most transmembrane helix-helix interfaces of a set of crystallized membrane proteins are structurally equivalent to soluble leucine zipper interaction domains. To establish a simplified model of these membrane-spanning leucine zippers, we studied the homophilic interactions of artificial transmembrane segments using different experimental approaches. Importantly, an oligoleucine, but not an oligoalanine, se- quence efficiently self-assembled in membranes as well as in detergent solution. Self-assembly was maintained when a leucine zipper type of heptad motif consisting of leucine residues was grafted onto an alanine host sequence. Analysis of point mutants or of a random sequence confirmed that the heptad motif of leucines mediates self-recognition of our artificial transmembrane segments. Further, a data base search identified degenerate versions of this leucine motif within transmembrane segments of a variety of functionally different proteins. For several of these natural transmembrane segments, self-interaction was experimentally verified. These results support various lines of previously reported evidence where these transmembrane segments were implicated in the oligomeric assembly of the corresponding proteins.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				W. Liu, M. Kawahara, H. Ueda, and T. Nagamune The Influence of Domain Structures on the Signal Transduction of Chimeric Receptors Derived from the Erythropoietin Receptor J. Biochem., May 1, 2009; 145(5): 575 - 584. [Abstract] [Full Text] [PDF]

				P. Hermand, F. Pincet, S. Carvalho, H. Ansanay, E. Trinquet, M. Daoudi, C. Combadiere, and P. Deterre Functional Adhesiveness of the CX3CL1 Chemokine Requires Its Aggregation: ROLE OF THE TRANSMEMBRANE DOMAIN J. Biol. Chem., October 31, 2008; 283(44): 30225 - 30234. [Abstract] [Full Text] [PDF]

				J. Lee and B. Sugden A Membrane Leucine Heptad Contributes to Trafficking, Signaling, and Transformation by Latent Membrane Protein 1 J. Virol., September 1, 2007; 81(17): 9121 - 9130. [Abstract] [Full Text] [PDF]

				N. Sal-Man, D. Gerber, I. Bloch, and Y. Shai Specificity in Transmembrane Helix-Helix Interactions Mediated by Aromatic Residues J. Biol. Chem., July 6, 2007; 282(27): 19753 - 19761. [Abstract] [Full Text] [PDF]

				N. Yang, X. Wang, J. Jiang, and S. J. Frank Role of the Growth Hormone (GH) Receptor Transmembrane Domain in Receptor Predimerization and GH-Induced Activation Mol. Endocrinol., July 1, 2007; 21(7): 1642 - 1655. [Abstract] [Full Text] [PDF]

				W. Duckwitz, R. Hausmann, A. Aschrafi, and G. Schmalzing P2X5 Subunit Assembly Requires Scaffolding by the Second Transmembrane Domain and a Conserved Aspartate J. Biol. Chem., December 22, 2006; 281(51): 39561 - 39572. [Abstract] [Full Text] [PDF]

				D. Clarke, S. Griffin, L. Beales, C. St. Gelais, S. Burgess, M. Harris, and D. Rowlands Evidence for the Formation of a Heptameric Ion Channel Complex by the Hepatitis C Virus P7 Protein in Vitro J. Biol. Chem., December 1, 2006; 281(48): 37057 - 37068. [Abstract] [Full Text] [PDF]

				F. H. Login and V. E. Shevchik The Single Transmembrane Segment Drives Self-assembly of OutC and the Formation of a Functional Type II Secretion System in Erwinia chrysanthemi J. Biol. Chem., November 3, 2006; 281(44): 33152 - 33162. [Abstract] [Full Text] [PDF]

				X. Mo, N. Lu, A. Padilla, J. A. Lopez, and R. Li The Transmembrane Domain of Glycoprotein Ibbeta Is Critical to Efficient Expression of Glycoprotein Ib-IX Complex in the Plasma Membrane J. Biol. Chem., August 11, 2006; 281(32): 23050 - 23059. [Abstract] [Full Text] [PDF]

				N. A. Noordeen, F. Carafoli, E. Hohenester, M. A. Horton, and B. Leitinger A Transmembrane Leucine Zipper Is Required for Activation of the Dimeric Receptor Tyrosine Kinase DDR1 J. Biol. Chem., August 11, 2006; 281(32): 22744 - 22751. [Abstract] [Full Text] [PDF]

				X. Lu, A. W. Gross, and H. F. Lodish Active Conformation of the Erythropoietin Receptor: RANDOM AND CYSTEINE-SCANNING MUTAGENESIS OF THE EXTRACELLULAR JUXTAMEMBRANE AND TRANSMEMBRANE DOMAINS J. Biol. Chem., March 17, 2006; 281(11): 7002 - 7011. [Abstract] [Full Text] [PDF]

				K. F. Kubatzky, W. Liu, K. Goldgraben, C. Simmerling, S. O. Smith, and S. N. Constantinescu Structural Requirements of the Extracellular to Transmembrane Domain Junction for Erythropoietin Receptor Function J. Biol. Chem., April 15, 2005; 280(15): 14844 - 14854. [Abstract] [Full Text] [PDF]

				M. Vooijs, E. H. Schroeter, Y. Pan, M. Blandford, and R. Kopan Ectodomain Shedding and Intramembrane Cleavage of Mammalian Notch Proteins Are Not Regulated through Oligomerization J. Biol. Chem., December 3, 2004; 279(49): 50864 - 50873. [Abstract] [Full Text] [PDF]

				O. Teijido, A. Martinez, M. Pusch, A. Zorzano, E. Soriano, J. A. del Rio, M. Palacin, and R. Estevez Localization and functional analyses of the MLC1 protein involved in megalencephalic leukoencephalopathy with subcortical cysts Hum. Mol. Genet., November 1, 2004; 13(21): 2581 - 2594. [Abstract] [Full Text] [PDF]

				M. Kawahara, Y. Ogo, H. Ueda, and T. Nagamune Improved growth response of antibody/receptor chimera attained by the engineering of transmembrane domain Protein Eng. Des. Sel., October 1, 2004; 17(10): 715 - 719. [Abstract] [Full Text] [PDF]

				C. Lambert, S. Mann, and R. Prange Assessment of determinants affecting the dual topology of hepadnaviral large envelope proteins J. Gen. Virol., May 1, 2004; 85(5): 1221 - 1225. [Abstract] [Full Text] [PDF]

				H. H. Sitte, H. Farhan, and J. A. Javitch Sodium-Dependent Neurotransmitter TRANSPORTERS: OLIGOMERIZATION as a Determinant of Transporter Function and Trafficking Mol. Interv., February 1, 2004; 4(1): 38 - 47. [Abstract] [Full Text] [PDF]

				W. Ruan, V. Becker, U. Klingmuller, and D. Langosch The Interface between Self-assembling Erythropoietin Receptor Transmembrane Segments Corresponds to a Membrane-spanning Leucine Zipper J. Biol. Chem., January 30, 2004; 279(5): 3273 - 3279. [Abstract] [Full Text] [PDF]

				S. N. Constantinescu, T. Keren, W. P. Russ, I. Ubarretxena-Belandia, Y. Malka, K. F. Kubatzky, D. M. Engelman, H. F. Lodish, and Y. I. Henis The Erythropoietin Receptor Transmembrane Domain Mediates Complex Formation with Viral Anemic and Polycythemic gp55 Proteins J. Biol. Chem., October 31, 2003; 278(44): 43755 - 43763. [Abstract] [Full Text] [PDF]

				M. Kawahara, H. Ueda, S. Morita, K. Tsumoto, I. Kumagai, and T. Nagamune Bypassing antibiotic selection: positive screening of genetically modified cells with an antigen-dependent proliferation switch Nucleic Acids Res., April 1, 2003; 31(7): e32 - e32. [Abstract] [Full Text] [PDF]

				P. Scholze, M. Freissmuth, and H. H. Sitte Mutations within an Intramembrane Leucine Heptad Repeat Disrupt Oligomer Formation of the Rat GABA Transporter 1 J. Biol. Chem., November 8, 2002; 277(46): 43682 - 43690. [Abstract] [Full Text] [PDF]

				A. Kaykas, K. Worringer, and B. Sugden LMP-1's Transmembrane Domains Encode Multiple Functions Required for LMP-1's Efficient Signaling J. Virol., October 11, 2002; 76(22): 11551 - 11560. [Abstract] [Full Text] [PDF]

				E. V. L. Grgacic Identification of structural determinants of the first transmembrane domain of the small envelope protein of duck hepatitis B virus essential for particle morphogenesis J. Gen. Virol., June 1, 2002; 83(7): 1635 - 1644. [Abstract] [Full Text] [PDF]

				J. M. Mendrola, M. B. Berger, M. C. King, and M. A. Lemmon The Single Transmembrane Domains of ErbB Receptors Self-associate in Cell Membranes J. Biol. Chem., February 8, 2002; 277(7): 4704 - 4712. [Abstract] [Full Text] [PDF]

				R. Gurezka and D. Langosch In Vitro Selection of Membrane-spanning Leucine Zipper Protein-Protein Interaction Motifs Using POSSYCCAT J. Biol. Chem., November 30, 2001; 276(49): 45580 - 45587. [Abstract] [Full Text] [PDF]

				A. J. Edgar, E. J. Birks, M. H. Yacoub, and J. M. Polak Cloning of Dexamethasone-Induced Transcript . A Novel Glucocorticoid-Induced Gene that Is Upregulated in Emphysema Am. J. Respir. Cell Mol. Biol., July 1, 2001; 25(1): 119 - 124. [Abstract] [Full Text] [PDF]

				S. N. Constantinescu, T. Keren, M. Socolovsky, H.-s. Nam, Y. I. Henis, and H. F. Lodish Ligand-independent oligomerization of cell-surface erythropoietin receptor is mediated by the transmembrane domain PNAS, April 10, 2001; 98(8): 4379 - 4384. [Abstract] [Full Text] [PDF]

				M. S. McClain, P. Cao, and T. L. Cover Amino-Terminal Hydrophobic Region of Helicobacter pylori Vacuolating Cytotoxin (VacA) Mediates Transmembrane Protein Dimerization Infect. Immun., February 1, 2001; 69(2): 1181 - 1184. [Abstract] [Full Text] [PDF]

				O Huber, R Kemler, and D Langosch Mutations affecting transmembrane segment interactions impair adhesiveness of E-cadherin J. Cell Sci., January 12, 1999; 112(23): 4415 - 4423. [Abstract] [PDF]

				D. Langosch, B. Brosig, and R. Pipkorn Peptide Mimics of the Vesicular Stomatitis Virus G-protein Transmembrane Segment Drive Membrane Fusion in Vitro J. Biol. Chem., August 17, 2001; 276(34): 32016 - 32021. [Abstract] [Full Text] [PDF]

				R. Laage, J. Rohde, B. Brosig, and D. Langosch A Conserved Membrane-spanning Amino Acid Motif Drives Homomeric and Supports Heteromeric Assembly of Presynaptic SNARE Proteins J. Biol. Chem., June 2, 2000; 275(23): 17481 - 17487. [Abstract] [Full Text] [PDF]