| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 14, 9305-9311, April 2, 1999
From the Departments of Biology and Kinesiology and Health Science,
York University, Toronto, Ontario M3J 1P3, Canada
Mitochondrial biogenesis can occur rapidly in
mammalian skeletal muscle subjected to a variety of physiological
conditions. However, the intracellular signal(s) involved in regulating
this process remain unknown. Using nuclearly encoded cytochrome
c, we show that its expression in muscle cells is increased
by changes in cytosolic Ca2+ using the ionophore A23187.
Treatment of myotubes with A23187 increased cytochrome c
mRNA expression up to 1.7-fold. Transfection experiments
using promoter-chloramphenicol acetyltransferase constructs revealed that this increase could be transcriptionally mediated since
A23187 increased chloramphenicol acetyltransferase activity by
2.5-fold. This increase was not changed by KN62, an inhibitor of
Ca2+/calmodulin-dependent kinases II and IV,
and it was not modified by overexpression of protein kinase A and cAMP
response element-binding protein, demonstrating that the A23187 effect
was not mediated through
Ca2+/calmodulin-dependent kinase- or protein
kinase A-dependent pathways. However, treatment of myotubes
with staurosporine or 12-O-tetradecanoylphorbol-13-acetate reduced the effect of A23187 on cytochrome c
transactivation by 40-50%. Coexpression of the
Ca2+-sensitive protein kinase C isoforms 
and
II, but not the Ca2+-insensitive 
isoform, exaggerated the A23187-mediated response. The short-term
effect of A23187 was mediated in part by mitogen-activated protein
kinase (extracellular signal-regulated kinases 1 and 2) since its
activation peaked 2 h after A23187 treatment, and cytochrome c transactivation was reduced by PD98089, a
mitogen-activated protein kinase/extracellular signal-regulated kinase
kinase inhibitor. These results demonstrate the existence of a
Ca2+-sensitive, protein kinase C-dependent
pathway involved in cytochrome c expression and implicate
Ca2+ as a signal in the up-regulation of nuclear genes
encoding mitochondrial proteins.
This article has been cited by other articles:
|
|
 |
|
  A. Franko, S. Mayer, G. Thiel, L. Mercy, T. Arnould, H.-T. Hornig-Do, R. J. Wiesner, and S. Goffart CREB-1{alpha} Is Recruited to and Mediates Upregulation of the Cytochrome c Promoter during Enhanced Mitochondrial Biogenesis Accompanying Skeletal Muscle Differentiation Mol. Cell. Biol., April 1, 2008; 28(7): 2446 - 2459. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Wright, P. C. Geiger, D.-H. Han, T. E. Jones, and J. O. Holloszy Calcium Induces Increases in Peroxisome Proliferator-activated Receptor {gamma} Coactivator-1{alpha} and Mitochondrial Biogenesis by a Pathway Leading to p38 Mitogen-activated Protein Kinase Activation J. Biol. Chem., June 29, 2007; 282(26): 18793 - 18799. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Devin and M. Rigoulet Mechanisms of mitochondrial response to variations in energy demand in eukaryotic cells Am J Physiol Cell Physiol, January 1, 2007; 292(1): C52 - C58. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Hood, I. Irrcher, V. Ljubicic, and A.-M. Joseph Coordination of metabolic plasticity in skeletal muscle J. Exp. Biol., June 15, 2006; 209(12): 2265 - 2275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Garcia-Roves, J. Huss, and J. O. Holloszy Role of calcineurin in exercise-induced mitochondrial biogenesis Am J Physiol Endocrinol Metab, June 1, 2006; 290(6): E1172 - E1179. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. R. Chin Role of Ca2+/calmodulin-dependent kinases in skeletal muscle plasticity J Appl Physiol, August 1, 2005; 99(2): 414 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Freyssenet, I. Irrcher, M. K. Connor, M. Di Carlo, and D. A. Hood Calcium-regulated changes in mitochondrial phenotype in skeletal muscle cells Am J Physiol Cell Physiol, May 1, 2004; 286(5): C1053 - C1061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Short, J. L. Vittone, M. L. Bigelow, D. N. Proctor, R. A. Rizza, J. M. Coenen-Schimke, and K. S. Nair Impact of Aerobic Exercise Training on Age-Related Changes in Insulin Sensitivity and Muscle Oxidative Capacity Diabetes, August 1, 2003; 52(8): 1888 - 1896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Irrcher, P. J. Adhihetty, T. Sheehan, A.-M. Joseph, and D. A. Hood PPARgamma coactivator-1alpha expression during thyroid hormone- and contractile activity-induced mitochondrial adaptations Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1669 - C1677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Carrasco, N. Riveros, J. Rios, M. Muller, F. Torres, J. Pineda, S. Lantadilla, and E. Jaimovich Depolarization-induced slow calcium transients activate early genes in skeletal muscle cells Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1438 - C1447. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. O. OJUKA, T. E. JONES, D.-H. HAN, M. CHEN, and J. O. HOLLOSZY Raising Ca2+ in L6 myotubes mimics effects of exercise on mitochondrial biogenesis in muscle FASEB J, April 1, 2003; 17(6): 675 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Colavecchia, L. N. Christie, Y. S. Kanwar, and D. A. Hood Functional consequences of thyroid hormone-induced changes in the mitochondrial protein import pathway Am J Physiol Endocrinol Metab, January 1, 2003; 284(1): E29 - E35. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Allen and L. A. Leinwand Intracellular Calcium and Myosin Isoform Transitions. CALCINEURIN AND CALCIUM-CALMODULIN KINASE PATHWAYS REGULATE PREFERENTIAL ACTIVATION OF THE IIa MYOSIN HEAVY CHAIN PROMOTER J. Biol. Chem., November 15, 2002; 277(47): 45323 - 45330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. O. Ojuka, T. E. Jones, D.-H. Han, M. Chen, B. R. Wamhoff, M. Sturek, and J. O. Holloszy Intermittent increases in cytosolic Ca2+ stimulate mitochondrial biogenesis in muscle cells Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E1040 - E1045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Duguez, L. Feasson, C. Denis, and D. Freyssenet Mitochondrial biogenesis during skeletal muscle regeneration Am J Physiol Endocrinol Metab, April 1, 2002; 282(4): E802 - E809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Chen, S. Carroll, P. Racay, J. Dick, D. Pette, I. Traub, G. Vrbova, P. Eggli, M. Celio, and B. Schwaller Deficiency in parvalbumin increases fatigue resistance in fast-twitch muscle and upregulates mitochondria Am J Physiol Cell Physiol, July 1, 2001; 281(1): C114 - C122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Hood Plasticity in Skeletal, Cardiac, and Smooth Muscle: Invited Review: Contractile activity-induced mitochondrial biogenesis in skeletal muscle J Appl Physiol, March 1, 2001; 90(3): 1137 - 1157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. W. Winder, B. F. Holmes, D. S. Rubink, E. B. Jensen, M. Chen, and J. O. Holloszy Activation of AMP-activated protein kinase increases mitochondrial enzymes in skeletal muscle J Appl Physiol, June 1, 2000; 88(6): 2219 - 2226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Connor, I. Irrcher, and D. A. Hood Contractile Activity-induced Transcriptional Activation of Cytochrome c Involves Sp1 and Is Proportional to Mitochondrial ATP Synthesis in C2C12 Muscle Cells J. Biol. Chem., May 4, 2001; 276(19): 15898 - 15904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Hallauer and K. E. M. Hastings Coregulation of fast contractile protein transgene and glycolytic enzyme expression in mouse skeletal muscle Am J Physiol Cell Physiol, January 1, 2002; 282(1): C113 - C124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. O. Ojuka, T. E. Jones, L. A. Nolte, M. Chen, B. R. Wamhoff, M. Sturek, and J. O. Holloszy Regulation of GLUT4 biogenesis in muscle: evidence for involvement of AMPK and Ca2+ Am J Physiol Endocrinol Metab, May 1, 2002; 282(5): E1008 - E1013. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
