|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 14, 9378-9385, April 2, 1999
From the Centre de recherche en cancérologie de
l'Université Laval, L'Hôtel-Dieu de Québec,
Québec, Québec G1R 2J6, Canada
Distinct biochemical activities have been
reported for small and large molecular complexes of heat shock protein
27 (HSP27), respectively. Using glycerol gradient ultracentrifugation
and chemical cross-linking, we show here that Chinese hamster HSP27 is
expressed in cells as homotypic multimers ranging from dimers up to
700-kDa oligomers. Treatments with arsenite, which induces phosphorylation on Ser15 and Ser90,
provoked a major change in the size distribution of the complexes that
shifted from oligomers to dimers. Ser90 phosphorylation was
sufficient and necessary for causing this change in structure. Dimer
formation was severely inhibited by replacing Ser90 with
Ala90 but not by replacing Ser15 with
Ala15. Using the yeast two-hybrid system, two domains were
identified that were responsible for HSP27 intermolecular interactions.
One domain was insensitive to phosphorylation and corresponded to the
C-terminal
HSP27 Multimerization Mediated by Phosphorylation-sensitive
Intermolecular Interactions at the Amino Terminus
-crystallin domain. The other domain was sensitive to
serine 90 phosphorylation and was located in the N-terminal region of
the protein. Fusion of this N-terminal domain to firefly luciferase
conferred luciferase with the capacity to form multimers that
dissociated into monomers upon phosphorylation. A deletion within this
domain of residues Arg5-Tyr23, which contains
a WDPF motif found in most proteins of the small heat shock protein
family, yielded a protein that forms only phosphorylation-insensitive dimers. We propose that HSP27 forms stable dimers through the -crystallin domain. These dimers further multimerize through intermolecular interactions mediated by the phosphorylation-sensitive N-terminal domain.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Hayes, V. Napoli, A. Mazurkie, W. F. Stafford, and P. Graceffa Phosphorylation Dependence of Hsp27 Multimeric Size and Molecular Chaperone Function J. Biol. Chem., July 10, 2009; 284(28): 18801 - 18807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Matsushima-Nishiwaki, S. Takai, S. Adachi, C. Minamitani, E. Yasuda, T. Noda, K. Kato, H. Toyoda, Y. Kaneoka, A. Yamaguchi, et al. Phosphorylated Heat Shock Protein 27 Represses Growth of Hepatocellular Carcinoma via Inhibition of Extracellular Signal-regulated Kinase J. Biol. Chem., July 4, 2008; 283(27): 18852 - 18860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Carra, S. J. Seguin, H. Lambert, and J. Landry HspB8 Chaperone Activity toward Poly(Q)-containing Proteins Depends on Its Association with Bag3, a Stimulator of Macroautophagy J. Biol. Chem., January 18, 2008; 283(3): 1437 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. MacIntyre, E. K. Tyson, M. Read, R. Smith, G. Yeo, K. Kwek, and E.-C. Chan Contraction in Human Myometrium Is Associated with Changes in Small Heat Shock Proteins Endocrinology, January 1, 2008; 149(1): 245 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Turakhia, C. D. Venkatakrishnan, K. Dunsmore, H. Wong, P. Kuppusamy, J. L. Zweier, and G. Ilangovan Doxorubicin-induced cardiotoxicity: direct correlation of cardiac fibroblast and H9c2 cell survival and aconitase activity with heat shock protein 27 Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H3111 - H3121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Alford, S. Glennie, B. R. Turrell, L. Rawlinson, J. Saklatvala, and J. L. E. Dean Heat Shock Protein 27 Functions in Inflammatory Gene Expression and Transforming Growth Factor-beta-activated Kinase-1 (TAK1)-mediated Signaling J. Biol. Chem., March 2, 2007; 282(9): 6232 - 6241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Venkatakrishnan, A. K. Tewari, L. Moldovan, A. J. Cardounel, J. L. Zweier, P. Kuppusamy, and G. Ilangovan Heat shock protects cardiac cells from doxorubicin-induced toxicity by activating p38 MAPK and phosphorylation of small heat shock protein 27 Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H2680 - H2691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Vertii, C. Hakim, A. Kotlyarov, and M. Gaestel Analysis of Properties of Small Heat Shock Protein Hsp25 in MAPK-activated Protein Kinase 2 (MK2)-deficient Cells: MK2-DEPENDENT INSOLUBILIZATION OF Hsp25 OLIGOMERS CORRELATES WITH SUSCEPTIBILITY TO STRESS J. Biol. Chem., September 15, 2006; 281(37): 26966 - 26975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Parhar, K. A. Baer, K. Parker, and M. J. Ropeleski Short-chain fatty acid mediated phosphorylation of heat shock protein 25: effects on camptothecin-induced apoptosis Am J Physiol Gastrointest Liver Physiol, August 1, 2006; 291(2): G178 - G188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Martin-Ventura, V. Nicolas, X. Houard, L. M. Blanco-Colio, A. Leclercq, J. Egido, R. Vranckx, J.-B. Michel, and O. Meilhac Biological Significance of Decreased HSP27 in Human Atherosclerosis Arterioscler. Thromb. Vasc. Biol., June 1, 2006; 26(6): 1337 - 1343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Giese, E. Basha, B. Y. Catague, and E. Vierling Evidence for an essential function of the N terminus of a small heat shock protein in vivo, independent of in vitro chaperone activity PNAS, December 27, 2005; 102(52): 18896 - 18901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Maulik Effect of p38 MAP kinase on cellular events during ischemia and reperfusion: possible therapy Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2302 - H2303. [Full Text] [PDF]
|
|
|
|
|
|
T. Okada, H. Otani, Y. Wu, S. Kyoi, C. Enoki, H. Fujiwara, T. Sumida, R. Hattori, and H. Imamura Role of F-actin organization in p38 MAP kinase-mediated apoptosis and necrosis in neonatal rat cardiomyocytes subjected to simulated ischemia and reoxygenation Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2310 - H2318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Berkowitz, P. Hu, Z. Liu, L. A. Diaz, J. J. Enghild, M. P. Chua, and D. S. Rubenstein Desmosome Signaling: INHIBITION OF p38MAPK PREVENTS PEMPHIGUS VULGARIS IgG-INDUCED CYTOSKELETON REORGANIZATION J. Biol. Chem., June 24, 2005; 280(25): 23778 - 23784. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Carra, M. Sivilotti, A. T. Chavez Zobel, H. Lambert, and J. Landry HspB8, a small heat shock protein mutated in human neuromuscular disorders, has in vivo chaperone activity in cultured cells Hum. Mol. Genet., June 15, 2005; 14(12): 1659 - 1669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. McMullen, P. W. Bryant, C. C. Glembotski, P. A. Vincent, and K. M. Pumiglia Activation of p38 Has Opposing Effects on the Proliferation and Migration of Endothelial Cells J. Biol. Chem., June 3, 2005; 280(22): 20995 - 21003. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hirano, X. Sun, C. A. DeGuzman, R. F. Ransom, K. R. McLeish, W. E. Smoyer, E. A. Shelden, M. J. Welsh, and R. Benndorf p38 MAPK/HSP25 signaling mediates cadmium-induced contraction of mesangial cells and renal glomeruli Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1133 - F1143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Doppler, P. Storz, J. Li, M. J. Comb, and A. Toker A Phosphorylation State-specific Antibody Recognizes Hsp27, a Novel Substrate of Protein Kinase D J. Biol. Chem., April 15, 2005; 280(15): 15013 - 15019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Chen, H.-Y. Zhang, K. Pavlish, and J. N. Benoit Effects of chronic portal hypertension on small heat-shock proteins in mesenteric arteries Am J Physiol Gastrointest Liver Physiol, April 1, 2005; 288(4): G616 - G620. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B G White, S J Williams, K Highmore, and D J MacPhee Small heat shock protein 27 (Hsp27) expression is highly induced in rat myometrium during late pregnancy and labour Reproduction, January 1, 2005; 129(1): 115 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Borbiev, A. Birukova, F. Liu, S. Nurmukhambetova, W. T. Gerthoffer, J. G. N. Garcia, and A. D. Verin p38 MAP kinase-dependent regulation of endothelial cell permeability Am J Physiol Lung Cell Mol Physiol, November 1, 2004; 287(5): L911 - L918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Giese and E. Vierling Mutants in a Small Heat Shock Protein That Affect the Oligomeric State: ANALYSIS AND ALLELE-SPECIFIC SUPPRESSION J. Biol. Chem., July 30, 2004; 279(31): 32674 - 32683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. P. Roux and J. Blenis ERK and p38 MAPK-Activated Protein Kinases: a Family of Protein Kinases with Diverse Biological Functions Microbiol. Mol. Biol. Rev., June 1, 2004; 68(2): 320 - 344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Theriault, H. Lambert, A. T. Chavez-Zobel, G. Charest, P. Lavigne, and J. Landry Essential Role of the NH2-terminal WD/EPF Motif in the Phosphorylation-activated Protective Function of Mammalian Hsp27 J. Biol. Chem., May 28, 2004; 279(22): 23463 - 23471. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pichon, M. Bryckaert, and E. Berrou Control of actin dynamics by p38 MAP kinase - Hsp27 distribution in the lamellipodium of smooth muscle cells J. Cell Sci., May 15, 2004; 117(12): 2569 - 2577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Sun, J.-M. Fontaine, J. S. Rest, E. A. Shelden, M. J. Welsh, and R. Benndorf Interaction of Human HSP22 (HSPB8) with Other Small Heat Shock Proteins J. Biol. Chem., January 23, 2004; 279(4): 2394 - 2402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Pasta, B. Raman, T. Ramakrishna, and Ch. M. Rao Role of the Conserved SRLFDQFFG Region of {alpha}-Crystallin, a Small Heat Shock Protein: EFFECT ON OLIGOMERIC SIZE, SUBUNIT EXCHANGE, AND CHAPERONE-LIKE ACTIVITY J. Biol. Chem., December 19, 2003; 278(51): 51159 - 51166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Doerwald, C. Onnekink, S. T. van Genesen, W. W. de Jong, and N. H. Lubsen Translational Thermotolerance Provided by Small Heat Shock Proteins Is Limited to Cap-dependent Initiation and Inhibited by 2-Aminopurine J. Biol. Chem., December 12, 2003; 278(50): 49743 - 49750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Aoudjit, M. Stanciu, H. Li, S. Lemay, and T. Takano p38 Mitogen-activated protein kinase protects glomerular epithelial cells from complement-mediated cell injury Am J Physiol Renal Physiol, October 1, 2003; 285(4): F765 - F774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Singh, D. W. Powell, M. J. Rane, T. H. Millard, J. O. Trent, W. M. Pierce, J. B. Klein, L. M. Machesky, and K. R. McLeish Identification of the p16-Arc Subunit of the Arp 2/3 Complex as a Substrate of MAPK-activated Protein Kinase 2 by Proteomic Analysis J. Biol. Chem., September 19, 2003; 278(38): 36410 - 36417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-J. Park, R. B. Gaynor, and Y. T. Kwak Heat Shock Protein 27 Association with the I{kappa}B Kinase Complex Regulates Tumor Necrosis Factor {alpha}-induced NF-{kappa}B Activation J. Biol. Chem., September 12, 2003; 278(37): 35272 - 35278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Chavez Zobel, A. Loranger, N. Marceau, J. R. Theriault, H. Lambert, and J. Landry Distinct chaperone mechanisms can delay the formation of aggresomes by the myopathy-causing R120G {alpha}B-crystallin mutant Hum. Mol. Genet., July 1, 2003; 12(13): 1609 - 1620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Koteiche and H. S. Mchaourab Mechanism of Chaperone Function in Small Heat-shock Proteins. PHOSPHORYLATION-INDUCED ACTIVATION OF TWO-MODE BINDING IN alpha B-CRYSTALLIN J. Biol. Chem., March 14, 2003; 278(12): 10361 - 10367. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Webster Serine Phosphorylation and Suppression of Apoptosis by the Small Heat Shock Protein {alpha}B-Crystallin Circ. Res., February 7, 2003; 92(2): 130 - 132. [Full Text] [PDF]
|
|
|
|
|
|
H. Sakamoto, T. Mashima, K. Yamamoto, and T. Tsuruo Modulation of Heat-shock Protein 27 (Hsp27) Anti-apoptotic Activity by Methylglyoxal Modification J. Biol. Chem., November 22, 2002; 277(48): 45770 - 45775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Bova, Q. Huang, L. Ding, and J. Horwitz Subunit Exchange, Conformational Stability, and Chaperone-like Function of the Small Heat Shock Protein 16.5 from Methanococcus jannaschii J. Biol. Chem., October 4, 2002; 277(41): 38468 - 38475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Decker, M. L. Decker, S. Nakamura, Y.-S. Zhao, S. Hedjbeli, K. R. Harris, and F. J. Klocke HSC73-tubulin complex formation during low-flow ischemia in the canine myocardium Am J Physiol Heart Circ Physiol, October 1, 2002; 283(4): H1322 - H1333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Eaton, W. Fuller, and M. J. Shattock S-Thiolation of HSP27 Regulates Its Multimeric Aggregate Size Independently of Phosphorylation J. Biol. Chem., June 7, 2002; 277(24): 21189 - 21196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Narberhaus {alpha}-Crystallin-Type Heat Shock Proteins: Socializing Minichaperones in the Context of a Multichaperone Network Microbiol. Mol. Biol. Rev., March 1, 2002; 66(1): 64 - 93. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. E. SMOYER and R. F. RANSOM Hsp27 regulates podocyte cytoskeletal changes in an in vitro model of podocyte process retraction FASEB J, March 1, 2002; 16(3): 315 - 326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Yeh, Y.-M. Chen, and C.-Y. Lin Functional Regions of Rice Heat Shock Protein, Oshsp16.9, Required for Conferring Thermotolerance in Escherichia coli Plant Physiology, February 1, 2002; 128(2): 661 - 668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Meier, G. L. King, A. Clermont, A. Perez, M. Hayashi, and E. P. Feener Angiotensin AT1 Receptor Stimulates Heat Shock Protein 27 Phosphorylation In Vitro and In Vivo Hypertension, December 1, 2001; 38(6): 1260 - 1265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jia, R. F. Ransom, M. Shibanuma, C. Liu, M. J. Welsh, and W. E. Smoyer Identification and Characterization of hic-5/ARA55 as an hsp27 Binding Protein J. Biol. Chem., October 19, 2001; 276(43): 39911 - 39918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. O. Hannigan, L. Zhan, Y. Ai, A. Kotlyarov, M. Gaestel, and C.-K. Huang Abnormal Migration Phenotype of Mitogen-Activated Protein Kinase-Activated Protein Kinase 2-/- Neutrophils in Zigmond Chambers Containing Formyl-Methionyl-Leucyl-Phenylalanine Gradients J. Immunol., October 1, 2001; 167(7): 3953 - 3961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. T. Gerthoffer and S. J. Gunst Signal Transduction in Smooth Muscle: Invited Review: Focal adhesion and small heat shock proteins in the regulation of actin remodeling and contractility in smooth muscle J Appl Physiol, August 1, 2001; 91(2): 963 - 972. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Kyriakis and J. Avruch Mammalian Mitogen-Activated Protein Kinase Signal Transduction Pathways Activated by Stress and Inflammation Physiol Rev, April 1, 2001; 81(2): 807 - 869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Jiang and S. Altman Protein-protein interactions with subunits of human nuclear RNase P PNAS, January 10, 2001; (2001) 21561498. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. M. Bany and G. A. Schultz Increased Expression of a Novel Heat Shock Protein Transcript in the Mouse Uterus During Decidualization and in Response to Progesterone Biol Reprod, January 1, 2001; 64(1): 284 - 292. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. J. Charette, J. N. Lavoie, H. Lambert, and J. Landry Inhibition of Daxx-Mediated Apoptosis by Heat Shock Protein 27 Mol. Cell. Biol., October 15, 2000; 20(20): 7602 - 7612. [Abstract] [Full Text]
|
|
|
|
|
|
M. Lasa, K. R. Mahtani, A. Finch, G. Brewer, J. Saklatvala, and A. R. Clark Regulation of Cyclooxygenase 2 mRNA Stability by the Mitogen-Activated Protein Kinase p38 Signaling Cascade Mol. Cell. Biol., June 15, 2000; 20(12): 4265 - 4274. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Cuesta, G. Laroia, and R. J. Schneider Chaperone Hsp27 inhibits translation during heat shock by binding eIF4G and facilitating dissociation of cap-initiation complexes Genes & Dev., June 15, 2000; 14(12): 1460 - 1470. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Kacimi, J. Chentoufi, N. Honbo, C. S. Long, and J. S. Karliner Hypoxia differentially regulates stress proteins in cultured cardiomyocytes: Role of the p38 stress-activated kinase signaling cascade, and relation to cytoprotection Cardiovasc Res, April 1, 2000; 46(1): 139 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sugiyama, A. Suzuki, M. Kishikawa, R. Akutsu, T. Hirose, M. M. Y. Waye, S. K. W. Tsui, S. Yoshida, and S. Ohno Muscle Develops a Specific Form of Small Heat Shock Protein Complex Composed of MKBP/HSPB2 and HSPB3 during Myogenic Differentiation J. Biol. Chem., January 14, 2000; 275(2): 1095 - 1104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Dorion, J. Berube, J. Huot, and J. Landry A Short Lived Protein Involved in the Heat Shock Sensing Mechanism Responsible for Stress-activated Protein Kinase 2 (SAPK2/p38) Activation J. Biol. Chem., December 31, 1999; 274(53): 37591 - 37597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bellmann, V. Burkart, J. Bruckhoff, H. Kolb, and J. Landry p38-dependent Enhancement of Cytokine-induced Nitric-oxide Synthase Gene Expression by Heat Shock Protein 70 J. Biol. Chem., June 9, 2000; 275(24): 18172 - 18179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Morrow, Y. Inaguma, K. Kato, and R. M. Tanguay The Small Heat Shock Protein Hsp22 of Drosophila melanogaster Is a Mitochondrial Protein Displaying Oligomeric Organization J. Biol. Chem., September 29, 2000; 275(40): 31204 - 31210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Studer and F. Narberhaus Chaperone Activity and Homo- and Hetero-oligomer Formation of Bacterial Small Heat Shock Proteins J. Biol. Chem., November 17, 2000; 275(47): 37212 - 37218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ito, K. Kamei, I. Iwamoto, Y. Inaguma, D. Nohara, and K. Kato Phosphorylation-induced Change of the Oligomerization State of alpha B-crystallin J. Biol. Chem., February 9, 2001; 276(7): 5346 - 5352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Benndorf, X. Sun, R. R. Gilmont, K. J. Biederman, M. P. Molloy, C. W. Goodmurphy, H. Cheng, P. C. Andrews, and M. J. Welsh HSP22, a New Member of the Small Heat Shock Protein Superfamily, Interacts with Mimic of Phosphorylated HSP27 (3DHSP27) J. Biol. Chem., July 13, 2001; 276(29): 26753 - 26761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Jiang and S. Altman Protein-protein interactions with subunits of human nuclear RNase P PNAS, January 30, 2001; 98(3): 920 - 925. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |