JBC Invitrogen Ultrasensitive Cytokine Assays

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lu, Y.
Right arrow Articles by Stewart, T. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lu, Y.
Right arrow Articles by Stewart, T. A.

J Biol Chem, Vol. 274, Issue 15, 10047-10052, April 9, 1999

NSP1 Defines a Novel Family of Adaptor Proteins Linking Integrin and Tyrosine Kinase Receptors to the c-Jun N-terminal Kinase/Stress-activated Protein Kinase Signaling Pathway

Yanmei Lu, Jennifer Brush, and Timothy A. Stewart

From the Department of Endocrine Research, Genentech, Inc., South San Francisco, California 94080

As part of a program to further understand the mechanism by which extracellular signals are coordinated and cell-specific outcomes are generated, we have cloned a novel class of related adaptor molecules (NSP1, NSP2, and NSP3) and have characterized in more detail one of the members, NSP1. NSP1 has an Shc-related SH2 domain and a putative proline/serine-rich SH3 interaction domain. Treatment of cells with epidermal growth factor or insulin leads to NSP1 phosphorylation and increased association with a hypophosphorylated adaptor protein, p130Cas. In contrast, cell contact with fibronectin results in Cas phosphorylation and a transient dissociation of NSP1 from p130Cas. Increased expression of NSP1 in 293 cells induces activation of JNK1, but not of ERK2. Consistent with this observation, NSP1 increases the activity of an AP-1-containing promoter. Thus, we have described a novel family of adaptor proteins, one of which may be involved in the process by which receptor tyrosine kinase and integrin receptors control the c-Jun N-terminal kinase/stress-activated protein kinase pathway.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 Cancer Res.Home page
R. S. Schrecengost, R. B. Riggins, K. S. Thomas, M. S. Guerrero, and A. H. Bouton
Breast Cancer Antiestrogen Resistance-3 Expression Regulates Breast Cancer Cell Migration through Promotion of p130Cas Membrane Localization and Membrane Ruffling
Cancer Res., July 1, 2007; 67(13): 6174 - 6182.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Briknarova, F. Nasertorabi, M. L. Havert, E. Eggleston, D. W. Hoyt, C. Li, A. J. Olson, K. Vuori, and K. R. Ely
The Serine-rich Domain from Crk-associated Substrate (p130cas) Is a Four-helix Bundle
J. Biol. Chem., June 10, 2005; 280(23): 21908 - 21914.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
K. N. Felekkis, R. P. Narsimhan, R. Near, A. F. Castro, Y. Zheng, L. A. Quilliam, and A. Lerner
AND-34 Activates Phosphatidylinositol 3-Kinase and Induces Anti-Estrogen Resistance in a SH2 and GDP Exchange Factor-Like Domain-Dependent Manner
Mol. Cancer Res., January 1, 2005; 3(1): 32 - 41.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Dail, M. S. Kalo, J. A. Seddon, J.-F. Cote, K. Vuori, and E. B. Pasquale
SHEP1 Function in Cell Migration Is Impaired by a Single Amino Acid Mutation That Disrupts Association with the Scaffolding Protein Cas but Not with Ras GTPases
J. Biol. Chem., October 1, 2004; 279(40): 41892 - 41902.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. B. Riggins, L. A. Quilliam, and A. H. Bouton
Synergistic Promotion of c-Src Activation and Cell Migration by Cas and AND-34/BCAR3
J. Biol. Chem., July 18, 2003; 278(30): 28264 - 28273.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Sakakibara, Y. Ohba, K. Kurokawa, M. Matsuda, and S. Hattori
Novel function of Chat in controlling cell adhesion via Cas-Crk-C3G-pathway-mediated Rap1 activation
J. Cell Sci., March 14, 2003; 115(24): 4915 - 4924.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Sakakibara, S. Hattori, S. Nakamura, and T. Katagiri
A Novel Hematopoietic Adaptor Protein, Chat-H, Positively Regulates T Cell Receptor-mediated Interleukin-2 Production by Jurkat Cells
J. Biol. Chem., February 14, 2003; 278(8): 6012 - 6017.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. Cai, K. N. Felekkis, R. I. Near, G. M. O'Neill, J. M. van Seventer, E. A. Golemis, and A. Lerner
The GDP Exchange Factor AND-34 Is Expressed in B Cells, Associates With HEF1, and Activates Cdc42
J. Immunol., January 15, 2003; 170(2): 969 - 978.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. J. Fashena, M. B. Einarson, G. M. O'Neill, C. Patriotis, and E. A. Golemis
Dissection of HEF1-dependent functions in motility and transcriptional regulation
J. Cell Sci., January 1, 2002; 115(1): 99 - 111.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. M. O'Neill and E. A. Golemis
Proteolysis of the Docking Protein HEF1 and Implications for Focal Adhesion Dynamics
Mol. Cell. Biol., August 1, 2001; 21(15): 5094 - 5108.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. F. Law, G. M. O'Neill, S. J. Fashena, M. B. Einarson, and E. A. Golemis
The Docking Protein HEF1 Is an Apoptotic Mediator at Focal Adhesion Sites
Mol. Cell. Biol., July 15, 2000; 20(14): 5184 - 5195.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
A. Sakakibara and S. Hattori
Chat, a Cas/HEF1-associated Adaptor Protein That Integrates Multiple Signaling Pathways
J. Biol. Chem., February 25, 2000; 275(9): 6404 - 6410.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
A. Brinkman, S. van der Flier, E. M. Kok, and L. C. J. Dorssers
BCAR1, a Human Homologue of the Adapter Protein p130Cas, and Antiestrogen Resistance in Breast Cancer Cells
J Natl Cancer Inst, January 19, 2000; 92(2): 112 - 120.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. A. Navas, D. T. Baldwin, and T. A. Stewart
RIP2 Is a Raf1-activated Mitogen-activated Protein Kinase Kinase
J. Biol. Chem., November 19, 1999; 274(47): 33684 - 33690.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. C. Dodelet, C. Pazzagli, A. H. Zisch, C. A. Hauser, and E. B. Pasquale
A Novel Signaling Intermediate, SHEP1, Directly Couples Eph Receptors to R-Ras and Rap1A
J. Biol. Chem., November 5, 1999; 274(45): 31941 - 31946.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Gotoh, D. Cai, X. Tian, L. A. Feig, and A. Lerner
p130Cas Regulates the Activity of AND-34, a Novel Ral, Rap1, and R-Ras Guanine Nucleotide Exchange Factor
J. Biol. Chem., September 22, 2000; 275(39): 30118 - 30123.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. F. Rebhun, A. F. Castro, and L. A. Quilliam
Identification of Guanine Nucleotide Exchange Factors (GEFs) for the Rap1 GTPase. REGULATION OF MR-GEF BY M-Ras-GTP INTERACTION
J. Biol. Chem., November 3, 2000; 275(45): 34901 - 34908.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.