| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 15, 10474-10480, April 9, 1999
§,
,,, and¶**
From the A partial-length human cDNA with a predicted
amino acid sequence homologous to a previously described heparan
sulfate iduronyl 2-sulfotransferase (Kobayashi, M., Habuchi, H.,
Yoneda, M., Habuchi, O., and Kimata, K. (1997) J. Biol.
Chem. 272, 13980-13985) was obtained by searching the expressed
sequence-tagged data bank. Northern blot analysis was performed using
this homologous cDNA as a probe, which demonstrated ubiquitous
expression of messages of 5.1 and 2.0 kilobases in a number of human
tissues and in several human cancer cell lines. Since the human
lymphoma Raji cell line had the highest level of expression, it was
used to isolate a full-length cDNA clone. The full-length cDNA
was found to contain an open reading frame that predicted a type II
transmembrane protein composed of 406 amino acid residues.
The cDNA in a baculovirus expression vector was expressed in
Sf9 insect cells, and cell extracts were then incubated together
with 3'-phosphoadenosine 5'-phospho[35S]sulfate and
potential glycosaminoglycan acceptors. This demonstrated substantial
sulfotransferase activity with dermatan sulfate, a small degree of
activity with chondroitin sulfate, but no sulfotransferase activity
with desulfated N-resulfated heparin. Analysis of
[35S]sulfate-labeled disaccharide products of chondroitin
ABC, chondroitin AC, and chondroitin B lyase treatment demonstrated
that the enzyme only transferred sulfate to the 2-position of uronyl
residues, which were preponderantly iduronyl residues in dermatan
sulfate, but some lesser transfer to glucuronyl residues of chondroitin sulfate.
Department of Biology, Massachusetts
Institute of Technology, Cambridge, Massachusetts 02139, the
§ Edith Nourse Rogers Memorial Veterans Hospital,
Bedford, Massachusetts 01730, the ¶ Department of Medicine, Brigham and Women's
Hospital,
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
This article has been cited by other articles:
|
|
 |
|
  K. Akita, A. von Holst, Y. Furukawa, T. Mikami, K. Sugahara, and A. Faissner Expression of Multiple Chondroitin/Dermatan Sulfotransferases in the Neurogenic Regions of the Embryonic and Adult Central Nervous System Implies That Complex Chondroitin Sulfates Have a Role in Neural Stem Cell Maintenance Stem Cells, March 1, 2008; 26(3): 798 - 809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yamaguchi, S. Ohtake, K. Kimata, and O. Habuchi Molecular Cloning of Squid N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase and Synthesis of a Unique Chondroitin Sulfate Containing E D Hybrid Tetrasaccharide Structure by the Recombinant Enzyme Glycobiology, December 1, 2007; 17(12): 1365 - 1376. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhu, L. M. Stevens, and D. Stein Synthesis of the sulfate donor PAPS in either the Drosophila germline or somatic follicle cells can support embryonic dorsal-ventral axis formation Development, April 15, 2007; 134(8): 1465 - 1469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Xu, D. Song, L. C. Pedersen, and J. Liu Mutational Study of Heparan Sulfate 2-O-Sulfotransferase and Chondroitin Sulfate 2-O-Sulfotransferase J. Biol. Chem., March 16, 2007; 282(11): 8356 - 8367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yusa, K. Kitajima, and O. Habuchi N-Linked Oligosaccharides on Chondroitin 6-Sulfotransferase-1 Are Required for Production of the Active Enzyme, Golgi Localization, and Sulfotransferase Activity toward Keratan Sulfate J. Biol. Chem., July 21, 2006; 281(29): 20393 - 20403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mitsunaga, T. Mikami, S. Mizumoto, J. Fukuda, and K. Sugahara Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains in the Development of Cerebellum: SPATIOTEMPORAL REGULATION OF THE EXPRESSION OF CRITICAL DISULFATED DISACCHARIDES BY SPECIFIC SULFOTRANSFERASES J. Biol. Chem., July 14, 2006; 281(28): 18942 - 18952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ohtake, K. Kimata, and O. Habuchi Recognition of Sulfation Pattern of Chondroitin Sulfate by Uronosyl 2-O-Sulfotransferase J. Biol. Chem., November 25, 2005; 280(47): 39115 - 39123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Bao, M. S. G. Pavao, J. C. dos Santos, and K. Sugahara A Functional Dermatan Sulfate Epitope Containing Iduronate(2-O-sulfate){alpha}1-3GalNAc(6-O-sulfate) Disaccharide in the Mouse Brain: DEMONSTRATION USING A NOVEL MONOCLONAL ANTIBODY RAISED AGAINST DERMATAN SULFATE OF ASCIDIAN ASCIDIA NIGRA J. Biol. Chem., June 17, 2005; 280(24): 23184 - 23193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hikino, T. Mikami, A. Faissner, A.-C. E. S. Vilela-Silva, M. S. G. Pavao, and K. Sugahara Oversulfated Dermatan Sulfate Exhibits Neurite Outgrowth-promoting Activity toward Embryonic Mouse Hippocampal Neurons: IMPLICATIONS OF DERMATAN SULFATE IN NEURITOGENESIS IN THE BRAIN J. Biol. Chem., October 31, 2003; 278(44): 43744 - 43754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ohtake, K. Kimata, and O. Habuchi A Unique Nonreducing Terminal Modification of Chondroitin Sulfate by N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase J. Biol. Chem., October 3, 2003; 278(40): 38443 - 38452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yada, M. Gotoh, T. Sato, M. Shionyu, M. Go, H. Kaseyama, H. Iwasaki, N. Kikuchi, Y.-D. Kwon, A. Togayachi, et al. Chondroitin Sulfate Synthase-2: MOLECULAR CLONING AND CHARACTERIZATION OF A NOVEL HUMAN GLYCOSYLTRANSFERASE HOMOLOGOUS TO CHONDROITIN SULFATE GLUCURONYLTRANSFERASE, WHICH HAS DUAL ENZYMATIC ACTIVITIES J. Biol. Chem., August 8, 2003; 278(32): 30235 - 30247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sato, M. Gotoh, K. Kiyohara, T. Akashima, H. Iwasaki, A. Kameyama, H. Mochizuki, T. Yada, N. Inaba, A. Togayachi, et al. Differential Roles of Two N-Acetylgalactosaminyltransferases, CSGalNAcT-1, and a Novel Enzyme, CSGalNAcT-2. INITIATION AND ELONGATION IN SYNTHESIS OF CHONDROITIN SULFATE J. Biol. Chem., January 24, 2003; 278(5): 3063 - 3071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fukuda, N. Hiraoka, T. O. Akama, and M. N. Fukuda Carbohydrate-modifying Sulfotransferases: Structure, Function, and Pathophysiology J. Biol. Chem., December 14, 2001; 276(51): 47747 - 47750. [Full Text] [PDF]
|
|
|
|
|
|
X. Bai, J. R. Brown, A. Varki, and J. D. Esko Enhanced 3-O-sulfation of galactose in Asn-linked glycans and Maackia amurenesis lectin binding in a new Chinese hamster ovary cell line Glycobiology, August 1, 2001; 11(8): 621 - 632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Hasegawa, T. Torii, T. Kato, H. Miyajima, A. Furuhata, K. Nakayasu, A. Kanai, and O. Habuchi Decreased GlcNAc 6-O-Sulfotransferase Activity in the Cornea with Macular Corneal Dystrophy Invest. Ophthalmol. Vis. Sci., November 1, 2000; 41(12): 3670 - 3677. [Abstract] [Full Text]
|
|
|
|
|
|
S. Yamauchi, S. Mita, T. Matsubara, M. Fukuta, H. Habuchi, K. Kimata, and O. Habuchi Molecular Cloning and Expression of Chondroitin 4-Sulfotransferase J. Biol. Chem., March 17, 2000; 275(12): 8975 - 8981. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Prydz and K. Dalen Synthesis and sorting of proteoglycans J. Cell Sci., January 1, 2000; 113(2): 193 - 205. [Abstract] [PDF]
|
|
|
|
|
|
N. Hiraoka, H. Nakagawa, E. Ong, T. O. Akama, M. N. Fukuda, and M. Fukuda Molecular Cloning and Expression of Two Distinct Human Chondroitin 4-O-Sulfotransferases That Belong to the HNK-1 Sulfotransferase Gene Family J. Biol. Chem., June 23, 2000; 275(26): 20188 - 20196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Li, F. Gong, K. El Darwish, M. Jalkanen, and U. Lindahl Characterization of the D-Glucuronyl C5-epimerase Involved in the Biosynthesis of Heparin and Heparan Sulfate J. Biol. Chem., June 1, 2001; 276(23): 20069 - 20077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Uchimura, K. Kadomatsu, H. Nishimura, H. Muramatsu, E. Nakamura, N. Kurosawa, O. Habuchi, F. M. El-Fasakhany, Y. Yoshikai, and T. Muramatsu Functional Analysis of the Chondroitin 6-Sulfotransferase Gene in Relation to Lymphocyte Subpopulations, Brain Development, and Oversulfated Chondroitin Sulfates J. Biol. Chem., January 4, 2002; 277(2): 1443 - 1450. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
