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J Biol Chem, Vol. 274, Issue 15, 10523-10532, April 9, 1999
From the School of Biochemistry and Molecular Genetics, The
University of New South Wales, Sydney,
New South Wales 2052, Australia
Expression of yeast genes involved in
one-carbon metabolism is controlled by glycine, by
L-methionine, and by nitrogen sources. Here we report
a novel control element containing a core CTTCTT motif mediating the
glycine response, demonstrating that a protein binds this element, that
binding is modulated by tetrahydrofolate, and that folate is required
for the in vivo glycine response. In an heterologous
CYC1 promoter the region needed for the glycine response of
GCV2 (encoding the P-subunit of glycine decarboxylase) mediated repression that was relieved by glycine. It was also responsible for L-methionine control but not nitrogen
repression. GCV1 and GCV3 have an homologous
region in their promoters. The GCV1 region conferred a
glycine response on an heterologous promoter acting as a repressor or
activator depending on promoter context. A protein was identified
that bound to the glycine regulatory regions of GCV1
and GCV2 only if the CTTCTT motif was
intact. This protein protected a 17-base pair
CATCN7CTTCTT region of GCV2 that is conserved
between GCV1 and GCV2. Protein binding was
increased by tetrahydrofolate, and use of a fol1 deletion
mutant indicated the involvement of a folate in the in vivo
glycine response. Tetrahydrofolate or a derivative may act as a ligand
for the transcription factor controlling expression of one-carbon
metabolism genes.
Control of Expression of One-carbon Metabolism Genes of
Saccharomyces cerevisiae Is Mediated by a
Tetrahydrofolate-responsive Protein Binding to a Glycine Regulatory
Region Including a Core 5'-CTTCTT-3' Motif
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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