| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 15, 10571-10581, April 9, 1999
From the Adhesion of fibroblasts to extracellular matrices
via integrin receptors is accompanied by extensive cytoskeletal
rearrangements and intracellular signaling events. The protein kinase C
(PKC) family of serine/threonine kinases has been implicated in several integrin-mediated events including focal adhesion formation, cell spreading, cell migration, and cytoskeletal rearrangements. However, the mechanism by which PKC regulates integrin function is not known. To
characterize the role of PKC family kinases in mediating integrin-induced signaling, we monitored the effects of PKC inhibition on fibronectin-induced signaling events in Cos7 cells using
pharmacological and genetic approaches. We found that inhibition of
classical and novel isoforms of PKC by down-regulation with
12-0-tetradeconoyl-phorbol-13-acetate or overexpression of
dominant-negative mutants of PKC significantly reduced extracellular
regulated kinase 2 (Erk2) activation by fibronectin receptors in Cos7
cells. Furthermore, overexpression of constitutively active PKC
Protein Kinase C Regulates Integrin-induced Activation of the
Extracellular Regulated Kinase Pathway Upstream of Shc
,
Department of Cell Biology, Harvard Medical
School, Boston, Massachusetts 02115 and the § Department of
Molecular Biology, Yokohama City University School of Medicine, 3-9, Fuku-ura, Kanazawa-ku, Yokohama 236, Japan
,
PKC
, or PKC
was sufficient to rescue
12-0-tetradeconoyl-phorbol-13-acetate-mediated
down-regulation of Erk2 activation, and all three of these PKC isoforms
were activated following adhesion. PKC was required for maximal
activation of mitogen-activated kinase kinase 1, Raf-1, and Ras,
tyrosine phosphorylation of Shc, and Shc association with Grb2. PKC
inhibition does not appear to have a generalized effect on integrin
signaling, because it does not block integrin-induced focal adhesion
kinase or paxillin tyrosine phosphorylation. These results indicate
that PKC activity enhances Erk2 activation in response to fibronectin
by stimulating the Erk/mitogen-activated protein kinase pathway at an
early step upstream of Shc.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M.-A Oh, E.-S. Kang, S.-A. Lee, E.-O. Lee, Y.-B. Kim, S.-H. Kim, and J. W. Lee PKC{delta} and cofilin activation affects peripheral actin reorganization and cell-cell contact in cells expressing integrin {alpha}5 but not its tailless mutant J. Cell Sci., August 1, 2007; 120(15): 2717 - 2730. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Xu, Z. Xie, R. Reece, D. Pimental, and M.-H. Zou Uncoupling of Endothelial Nitric Oxidase Synthase by Hypochlorous Acid: Role of NAD(P)H Oxidase-Derived Superoxide and Peroxynitrite Arterioscler. Thromb. Vasc. Biol., December 1, 2006; 26(12): 2688 - 2695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Osmanagic-Myers, M. Gregor, G. Walko, G. Burgstaller, S. Reipert, and G. Wiche Plectin-controlled keratin cytoarchitecture affects MAP kinases involved in cellular stress response and migration J. Cell Biol., August 14, 2006; 174(4): 557 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zheng and P. J. McKeown-Longo Cell adhesion regulates Ser/Thr phosphorylation and proteasomal degradation of HEF1 J. Cell Sci., January 1, 2006; 119(1): 96 - 103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Rucci, C. DiGiacinto, L. Orru, D. Millimaggi, R. Baron, and A. Teti A novel protein kinase C {alpha}-dependent signal to ERK1/2 activated by {alpha}V{beta}3 integrin in osteoclasts and in Chinese hamster ovary (CHO) cells J. Cell Sci., August 1, 2005; 118(15): 3263 - 3275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Wheeler, P. J. Reddig, K. J. Ness, C. P. Leith, T. D. Oberley, and A. K. Verma Overexpression of Protein Kinase C-{epsilon} in the Mouse Epidermis Leads to a Spontaneous Myeloproliferative-Like Disease Am. J. Pathol., January 1, 2005; 166(1): 117 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Wheeler, K. E. Martin, K. J. Ness, Y. Li, N. E. Dreckschmidt, M. Wartman, H. N. Ananthaswamy, D. L. Mitchell, and A. K. Verma Protein Kinase C {epsilon} Is an Endogenous Photosensitizer That Enhances Ultraviolet Radiation-Induced Cutaneous Damage and Development of Squamous Cell Carcinomas1 Cancer Res., November 1, 2004; 64(21): 7756 - 7765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hasegawa, M. Fujitani, K. Hata, M. Tohyama, S. Yamagishi, and T. Yamashita Promotion of Axon Regeneration by Myelin-Associated Glycoprotein and Nogo through Divergent Signals Downstream of Gi/G J. Neurosci., July 28, 2004; 24(30): 6826 - 6832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. G. Choudhury A Linear Signal Transduction Pathway Involving Phosphatidylinositol 3-Kinase, Protein Kinase C{epsilon}, and MAPK in Mesangial Cells Regulates Interferon-{gamma}-induced STAT1{alpha} Transcriptional Activation J. Biol. Chem., June 25, 2004; 279(26): 27399 - 27409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. I. Willis, D. Pierre-Paul, B. E. Sumpio, and V. Gahtan Vascular Smooth Muscle Cell Migration: Current Research and Clinical Implications Vascular and Endovascular Surgery, January 1, 2004; 38(1): 11 - 23. [Abstract] [PDF]
|
|
|
|
|
|
Z. Xie, M. Singh, D. A. Siwik, W. L. Joyner, and K. Singh Osteopontin Inhibits Interleukin-1{beta}-stimulated Increases in Matrix Metalloproteinase Activity in Adult Rat Cardiac Fibroblasts: ROLE OF PROTEIN KINASE C-{zeta} J. Biol. Chem., December 5, 2003; 278(49): 48546 - 48552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Davis, J. Ronesi, and D. M. Lovinger A Predominant Role for Inhibition of the Adenylate Cyclase/Protein Kinase A Pathway in ERK Activation by Cannabinoid Receptor 1 in N1E-115 Neuroblastoma Cells J. Biol. Chem., December 5, 2003; 278(49): 48973 - 48980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Asghar, T. Hussain, and M. F. Lokhandwala Overexpression of PKC-{beta}I and -{delta} contributes to higher PKC activity in the proximal tubules of old Fischer 344 rats Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1100 - F1107. [Abstract] [Full Text]
|
|
|
|
|
|
D. L. Wheeler, K. J. Ness, T. D. Oberley, and A. K. Verma Protein Kinase C{epsilon} Is Linked to 12-O-tetradecanoylphorbol-13-acetate-induced Tumor Necrosis Factor-{alpha} Ectodomain Shedding and the Development of Metastatic Squamous Cell Carcinoma in Protein Kinase C{epsilon} Transgenic Mice Cancer Res., October 1, 2003; 63(19): 6547 - 6555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Mazzoni, A. Adam, E. Bal de Kier Joffe, and J. A. Aguirre-Ghiso Immortalized Mammary Epithelial Cells Overexpressing Protein Kinase C {gamma} Acquire a Malignant Phenotype and Become Tumorigenic in Vivo Mol. Cancer Res., August 1, 2003; 1(10): 776 - 787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, K. Chen, Y. Tu, A. Velyvis, Y. Yang, J. Qin, and C. Wu Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites J. Cell Sci., March 14, 2003; 115(24): 4777 - 4786. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Niu, H. Xie, and E. E. Marcantonio Integrin-mediated Tyrosine Phosphorylation of Shc in T Cells Is Regulated by Protein Kinase C-dependent Phosphorylations of Lck Mol. Biol. Cell, February 1, 2003; 14(2): 349 - 360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Liao, R. Bucala, and R. A. Mitchell Adhesion-dependent Signaling by Macrophage Migration Inhibitory Factor (MIF) J. Biol. Chem., January 3, 2003; 278(1): 76 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Q. Wang, P. Sun, and A. S. Paller Ganglioside Modulation Regulates Epithelial Cell Adhesion and Spreading via Ganglioside-specific Effects on Signaling J. Biol. Chem., October 18, 2002; 277(43): 40410 - 40419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mauro, C. Ciccarelli, P. De Cesaris, A. Scoglio, M. Bouche, M. Molinaro, A. Aquino, and B. M. Zani PKC{alpha}-mediated ERK, JNK and p38 activation regulates the myogenic program in human rhabdomyosarcoma cells J. Cell Sci., September 15, 2002; 115(18): 3587 - 3599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-H. Disatnik, S. C. Boutet, C. H. Lee, D. Mochly-Rosen, and T. A. Rando Sequential activation of individual PKC isozymes in integrin-mediated muscle cell spreading: a role for MARCKS in an integrin signaling pathway J. Cell Sci., May 15, 2002; 115(10): 2151 - 2163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. H. Ziegler, U. Tigges, A. Zieseniss, and B. M. Jockusch A Lipid-regulated Docking Site on Vinculin for Protein Kinase C J. Biol. Chem., February 22, 2002; 277(9): 7396 - 7404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Hyduk and M. I. Cybulsky {alpha}4 Integrin Signaling Activates Phosphatidylinositol 3-Kinase and Stimulates T Cell Adhesion to Intercellular Adhesion Molecule-1 to a Similar Extent As CD3, but Induces a Distinct Rearrangement of the Actin Cytoskeleton J. Immunol., January 15, 2002; 168(2): 696 - 704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Stupack and D. A. Cheresh Get a ligand, get a life: integrins, signaling and cell survival J. Cell Sci., January 10, 2002; 115(19): 3729 - 3738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Paruchuri, B. Hallberg, M. Juhas, C. Larsson, and A. Sjolander Leukotriene D4 activates MAPK through a Ras-independent but PKC{epsilon}-dependent pathway in intestinal epithelial cells J. Cell Sci., January 5, 2002; 115(9): 1883 - 1893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Stockton and B. S. Jacobson Modulation of Cell-Substrate Adhesion by Arachidonic Acid: Lipoxygenase Regulates Cell Spreading and ERK1/2-inducible Cyclooxygenase Regulates Cell Migration in NIH-3T3 Fibroblasts Mol. Biol. Cell, July 1, 2001; 12(7): 1937 - 1956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Alt, M. Ohba, L. Li, M. Gartsbein, A. Belanger, Mitchell. F. Denning, T. Kuroki, S. H. Yuspa, and T. Tennenbaum Protein Kinase C{{delta}}-mediated Phosphorylation of {{alpha}}6{beta}4 Is Associated with Reduced Integrin Localization to the Hemidesmosome and Decreased Keratinocyte Attachment Cancer Res., June 1, 2001; 61(11): 4591 - 4598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liu, C. Graham, V. Parravicini, M. J. Brown, J. Rivera, and S. Shaw Protein kinase C {theta} is expressed in mast cells and is functionally involved in Fc{varepsilon} receptor I signaling J. Leukoc. Biol., May 1, 2001; 69(5): 831 - 840. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Gao, S. Tang, S. Zhou, and J. A. Ware The Thromboxane A2 Receptor Activates Mitogen-Activated Protein Kinase via Protein Kinase C-Dependent Gi Coupling and Src-Dependent Phosphorylation of the Epidermal Growth Factor Receptor J. Pharmacol. Exp. Ther., April 13, 2001; 296(2): 426 - 433. [Abstract] [Full Text]
|
|
|
|
|
|
C. Buensuceso, D Woodside, J. Huff, G. Plopper, and T. O'Toole The WD protein Rack1 mediates protein kinase C and integrin-dependent cell migration J. Cell Sci., January 5, 2001; 114(9): 1691 - 1698. [Abstract] [PDF]
|
|
|
|
|
|
C. E. Ahnadi, P. Giguère, S. Gravel, D. Gagné, A.-C. Goulet, T. Fülöp Jr, M. D. Payet, and G. Dupuis Chronic PMA treatment of Jurkat T lymphocytes results in decreased protein tyrosine phosphorylation and inhibition of CD3- but not Ti-dependent antibody-triggered Ca2+ signaling J. Leukoc. Biol., August 1, 2000; 68(2): 293 - 300. [Abstract] [Full Text]
|
|
|
|
|
|
M. Fornaro, C. A. Steger, A. M. Bennett, J. J. Wu, and L. R. Languino Differential Role of beta 1C and beta 1A Integrin Cytoplasmic Variants in Modulating Focal Adhesion Kinase, Protein Kinase B/AKT, and Ras/Mitogen-activated Protein Kinase Pathways Mol. Biol. Cell, July 1, 2000; 11(7): 2235 - 2249. [Abstract] [Full Text]
|
|
|
|
|
|
J. Li, Y.-N. Kim, and P. J. Bertics Platelet-derived Growth Factor-stimulated Migration of Murine Fibroblasts Is Associated with Epidermal Growth Factor Receptor Expression and Tyrosine Phosphorylation J. Biol. Chem., January 28, 2000; 275(4): 2951 - 2958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Adams, J. D. Clelland, G. D.M. Collett, F. Matsumura, S. Yamashiro, and L. Zhang Cell-Matrix Adhesions Differentially Regulate Fascin Phosphorylation Mol. Biol. Cell, December 1, 1999; 10(12): 4177 - 4190. [Abstract] [Full Text]
|
|
|
|
|
|
L. Barberis, K. K. Wary, G. Fiucci, F. Liu, E. Hirsch, M. Brancaccio, F. Altruda, G. Tarone, and F. G. Giancotti Distinct Roles of the Adaptor Protein Shc and Focal Adhesion Kinase in Integrin Signaling to ERK J. Biol. Chem., November 17, 2000; 275(47): 36532 - 36540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Dong, J. S. Gutkind, and A. C. Larner Granulocyte Colony-stimulating Factor Induces Erk5 Activation, Which Is Differentially Regulated by Protein-tyrosine Kinases and Protein Kinase C. REGULATION OF CELL PROLIFERATION AND SURVIVAL J. Biol. Chem., March 30, 2001; 276(14): 10811 - 10816. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
