|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 15, 9930-9936, April 9, 1999
From the Using a set of parental and recombinant murine
hepatitis virus strains, we demonstrate that the nucleocapsid protein
induces transcription of the novel fgl2 prothrombinase gene
and elevated procoagulant activity in those strains that produce
fulminant hepatitis. Chinese hamster ovary cells cotransfected with a
construct expressing nucleocapsid protein from susceptible strains and
with a luciferase reporter construct containing the fgl2
promoter showed a 6-fold increase in luciferase activity compared with
nontransfected cells or cells cotransfected with a construct expressing
nucleocapsid protein from resistant strains. Two deletions found at
coding sites 111-123 and 1143-1145 of structural domains I and III,
respectively, of the nucleocapsid gene may account for the differences
between pathogenic and nonpathogenic strains. Preliminary mapping of
the fgl2 promoter has defined a region from
The Nucleocapsid Protein of Murine Hepatitis Virus Type 3 Induces Transcription of the Novel fgl2 Prothrombinase
Gene
,,,,,
,,,, and
Multi-Organ Transplant Program and
Departments of Medicine and Pathology, Toronto Hospital, St. Michael's
Hospital, and the University of Toronto, Toronto, Ontario M5G 2C4,
Canada, the § Department of Molecular Microbiology and
Immunology and the Howard Hughes Medical Institute, University of
Southern California School of Medicine, Los Angeles, California 90033, and the Department of Pathology, Texas A & M University,
College Station, Texas 77843
372 to 306
upstream from the ATG translation initiation site to be responsive to
nucleocapsid protein. Hence, mapping of genetic determinants in
parental and recombinant strains demonstrates that the nucleocapsid
protein of strains that induce fulminant hepatitis is responsible for transcription of the fgl2 prothrombinase gene. These
studies provide new insights into the role of the nucleocapsid gene in
the pathogenesis of viral hepatitis.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Frieman and R. Baric Mechanisms of Severe Acute Respiratory Syndrome Pathogenesis and Innate Immunomodulation Microbiol. Mol. Biol. Rev., December 1, 2008; 72(4): 672 - 685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Han, W. Yan, W. Guo, D. Xi, Y. Zhou, W. Li, S. Gao, M. Liu, G. Levy, X. Luo, et al. Hepatitis B Virus-induced hFGL2 Transcription Is Dependent on c-Ets-2 and MAPK Signal Pathway J. Biol. Chem., November 21, 2008; 283(47): 32715 - 32729. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Han, W. Yan, Y. Huang, H. Yao, Z. Wang, D. Xi, W. Li, Y. Zhou, J. Hou, X. Luo, et al. The Nucleocapsid Protein of SARS-CoV Induces Transcription of hfgl2 Prothrombinase Gene Dependent on C/EBP Alpha J. Biochem., July 1, 2008; 144(1): 51 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Shalev, H. Liu, C. Koscik, A. Bartczak, M. Javadi, K. M. Wong, A. Maknojia, W. He, M. F. Liu, J. Diao, et al. Targeted Deletion of fgl2 Leads to Impaired Regulatory T Cell Activity and Development of Autoimmune Glomerulonephritis J. Immunol., January 1, 2008; 180(1): 249 - 260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Reed, G. Howell, S. M. Harrison, K.-A. Spencer, and J. A. Hiscox Characterization of the Nuclear Export Signal in the Coronavirus Infectious Bronchitis Virus Nucleocapsid Protein J. Virol., April 15, 2007; 81(8): 4298 - 4304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Navas-Martin, M. Brom, M.-M. Chua, R. Watson, Z. Qiu, and S. R. Weiss Replicase Genes of Murine Coronavirus Strains A59 and JHM Are Interchangeable: Differences in Pathogenesis Map to the 3' One-Third of the Genome J. Virol., January 15, 2007; 81(2): 1022 - 1026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. De Albuquerque, E. Baig, X. Ma, J. Zhang, W. He, A. Rowe, M. Habal, M. Liu, I. Shalev, G. P. Downey, et al. Murine hepatitis virus strain 1 produces a clinically relevant model of severe acute respiratory syndrome in a/j mice. J. Virol., November 1, 2006; 80(21): 10382 - 10394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Weiss and S. Navas-Martin Coronavirus Pathogenesis and the Emerging Pathogen Severe Acute Respiratory Syndrome Coronavirus Microbiol. Mol. Biol. Rev., December 1, 2005; 69(4): 635 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Ning, Y. Sun, M. Han, L. Zhang, C. Zhu, W. Zhang, H. Guo, J. Li, W. Yan, F. Gong, et al. Role of Fibrinogen-Like Protein 2 Prothrombinase/Fibroleukin in Experimental and Human Allograft Rejection J. Immunol., June 1, 2005; 174(11): 7403 - 7411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Ning, S. Lakatoo, M. Liu, W. Yang, Z. Wang, M. J. Phillips, and G. A. Levy Induction of Prothrombinase fgl2 by the Nucleocapsid Protein of Virulent Mouse Hepatitis Virus Is Dependent on Host Hepatic Nuclear Factor-4alpha J. Biol. Chem., April 25, 2003; 278(18): 15541 - 15549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Navas and S. R. Weiss Murine Coronavirus-Induced Hepatitis: JHM Genetic Background Eliminates A59 Spike-Determined Hepatotropism J. Virol., April 15, 2003; 77(8): 4972 - 4978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Levy, M. Liu, J. Ding, S. Yuwaraj, J. Leibowitz, P. A. Marsden, Q. Ning, A. Kovalinka, and M. J. Phillips Molecular and Functional Analysis of the Human Prothrombinase Gene (HFGL2) and Its Role in Viral Hepatitis Am. J. Pathol., April 1, 2000; 156(4): 1217 - 1225. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |