| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 16, 10673-10676, April 16, 1999
From the Pharmacogenetics Section, Laboratory of Reproductive and
Developmental Toxicology, NIEHS, National Institutes of Health,
Research Triangle Park, North Carolina 27709
Heparan sulfate
N-deacetylase/N-sulfotransferase (HSNST)
catalyzes the first and obligatory step in the biosynthesis of heparan sulfates and heparin. The crystal structure of the sulfotransferase domain (NST1) of human HSNST-1 has been determined at 2.3-Å resolution in a binary complex with 3'-phosphoadenosine 5'-phosphate (PAP). NST1
is approximately spherical with an open cleft, and consists of a single
/
fold with a central five-stranded parallel -sheet and a
three-stranded anti-parallel -sheet bearing an interstrand disulfide
bond. The structural regions 1, 6, 1, 7, 5'-phosphosulfate binding loop (between 1 and 1), and a random coil (between 8 and 13) constitute the PAP binding site of NST1. The 6 and random coil (between 2 and 2), which form an open cleft near the
5'-phosphate of the PAP molecule, may provide interactions for
substrate binding. The conserved residue Lys-614 is in position to form
a hydrogen bond with the bridge oxygen of the 5'-phosphate.
This article has been cited by other articles:
|
|
 |
|
  D. Xu, D. Song, L. C. Pedersen, and J. Liu Mutational Study of Heparan Sulfate 2-O-Sulfotransferase and Chondroitin Sulfate 2-O-Sulfotransferase J. Biol. Chem., March 16, 2007; 282(11): 8356 - 8367. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Chen, K. Ichihara-Tanaka, and T. Muramatsu Role of the Carboxyl-Terminal Region in the Activity of N-Acetylglucosamine 6-O-Sulfotransferase-1 J. Biochem., November 1, 2004; 136(5): 659 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Moon, S. C. Edavettal, J. M. Krahn, E. M. Munoz, M. Negishi, R. J. Linhardt, J. Liu, and L. C. Pedersen Structural Analysis of the Sulfotransferase (3-O-Sulfotransferase Isoform 3) Involved in the Biosynthesis of an Entry Receptor for Herpes Simplex Virus 1 J. Biol. Chem., October 22, 2004; 279(43): 45185 - 45193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Edavettal, K. A. Lee, M. Negishi, R. J. Linhardt, J. Liu, and L. C. Pedersen Crystal Structure and Mutational Analysis of Heparan Sulfate 3-O-Sulfotransferase Isoform 1 J. Biol. Chem., June 11, 2004; 279(24): 25789 - 25797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Barnett, S. Tsvetanov, N. Gamage, J. L. Martin, R. G. Duggleby, and M. E. McManus Active Site Mutations and Substrate Inhibition in Human Sulfotransferase 1A1 and 1A3 J. Biol. Chem., April 30, 2004; 279(18): 18799 - 18805. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Pi, Y. Yu, J. D. Mougous, and J. A. Leary Observation of a hybrid random ping-pong mechanism of catalysis for NodST: A mass spectrometry approach Protein Sci., April 1, 2004; 13(4): 903 - 912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Jemth, E. Smeds, A.-T. Do, H. Habuchi, K. Kimata, U. Lindahl, and M. Kusche-Gullberg Oligosaccharide Library-based Assessment of Heparan Sulfate 6-O-Sulfotransferase Substrate Specificity J. Biol. Chem., June 27, 2003; 278(27): 24371 - 24376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. U. Gamage, R. G. Duggleby, A. C. Barnett, M. Tresillian, C. F. Latham, N. E. Liyou, M. E. McManus, and J. L. Martin Structure of a Human Carcinogen-converting Enzyme, SULT1A1. STRUCTURAL AND KINETIC IMPLICATIONS OF SUBSTRATE INHIBITION J. Biol. Chem., February 21, 2003; 278(9): 7655 - 7662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Chapman, M. C. Bryan, and C.-H. Wong Mechanistic studies of beta -arylsulfotransferase IV PNAS, February 4, 2003; 100(3): 910 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. van den Born, D. S. Pikas, B. J.M. Pisa, I. Eriksson, L. Kjellen, and J. H.M. Berden Antibody-based assay for N-deacetylase activity of heparan sulfate/heparin N-deacetylase/N-sulfotransferase (NDST): novel characteristics of NDST-1 and -2 Glycobiology, January 1, 2003; 13(1): 1 - 10. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Strott Sulfonation and Molecular Action Endocr. Rev., October 1, 2002; 23(5): 703 - 732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Pedersen, E. Petrotchenko, S. Shevtsov, and M. Negishi Crystal Structure of the Human Estrogen Sulfotransferase-PAPS Complex. EVIDENCE FOR CATALYTIC ROLE OF Ser137 IN THE SULFURYL TRANSFER REACTION J. Biol. Chem., May 10, 2002; 277(20): 17928 - 17932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Bell, A. Mavila, R. Salazar, K. J. Bayless, S. Kanagala, S. A. Maxwell, and G. E. Davis Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and G-protein signaling J. Cell Sci., January 8, 2001; 114(15): 2755 - 2773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Prydz and K. Dalen Synthesis and sorting of proteoglycans J. Cell Sci., January 1, 2000; 113(2): 193 - 205. [Abstract] [PDF]
|
|
|
|
|
|
E. V. Petrotchenko, M. E. Doerflein, Y. Kakuta, L. C. Pedersen, and M. Negishi Substrate Gating Confers Steroid Specificity to Estrogen Sulfotransferase J. Biol. Chem., October 15, 1999; 274(42): 30019 - 30022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ong, J.-C. Yeh, Y. Ding, O. Hindsgaul, L. C. Pedersen, M. Negishi, and M. Fukuda Structure and Function of HNK-1 Sulfotransferase. IDENTIFICATION OF DONOR AND ACCEPTOR BINDING SITES BY SITE-DIRECTED MUTAGENESIS J. Biol. Chem., September 3, 1999; 274(36): 25608 - 25612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hiraoka, H. Nakagawa, E. Ong, T. O. Akama, M. N. Fukuda, and M. Fukuda Molecular Cloning and Expression of Two Distinct Human Chondroitin 4-O-Sulfotransferases That Belong to the HNK-1 Sulfotransferase Gene Family J. Biol. Chem., June 23, 2000; 275(26): 20188 - 20196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Pedersen, K. Tsuchida, H. Kitagawa, K. Sugahara, T. A. Darden, and M. Negishi Heparan/Chondroitin Sulfate Biosynthesis. STRUCTURE AND MECHANISM OF HUMAN GLUCURONYLTRANSFERASE I J. Biol. Chem., October 27, 2000; 275(44): 34580 - 34585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Xia, M. R. Evers, H.-G. Kang, M. Schachner, and J. U. Baenziger Molecular Cloning and Expression of the Pituitary Glycoprotein Hormone N-Acetylgalactosamine-4-O-sulfotransferase J. Biol. Chem., December 1, 2000; 275(49): 38402 - 38409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-i. Aikawa, K. Grobe, M. Tsujimoto, and J. D Esko Multiple Isozymes of Heparan Sulfate/Heparin GlcNAc N-Deacetylase/GlcN N-Sulfotransferase. STRUCTURE AND ACTIVITY OF THE FOURTH MEMBER, NDST4 J. Biol. Chem., February 16, 2001; 276(8): 5876 - 5882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-G. Kang, M. R. Evers, G. Xia, J. U. Baenziger, and M. Schachner Molecular Cloning and Expression of an N-Acetylgalactosamine-4-O-sulfotransferase That Transfers Sulfate to Terminal and Non-terminal beta 1,4-Linked N-Acetylgalactosamine J. Biol. Chem., March 30, 2001; 276(14): 10861 - 10869. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
