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J Biol Chem, Vol. 274, Issue 16, 10802-10806, April 16, 1999

Direct EPR Detection of the Carbonate Radical Anion Produced from Peroxynitrite and Carbon Dioxide

Marcelo G. Bonini, Rafael Radi^§, Gerardo Ferrer-Sueta^¶, Ana M. Da C. Ferreira, and Ohara Augusto

From the  Departamento de Bioquímica and  Departamento de Química, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil 05599-970 and ^§ Departamento de Bioquimica, Facultad de Medicina and ^¶ Unidad Asociada Enzimologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay 11800

The biological effects of peroxynitrite have been recently considered to be largely dependent on its reaction with carbon dioxide, which is present in high concentrations in intra- and extracellular compartments. Peroxynitrite anion (ONOO) reacts rapidly with carbon dioxide, forming an adduct, nitrosoperoxocarboxylate (ONOOCO₂), whose decomposition has been proposed to produce reactive intermediates such as the carbonate radical (CO₃). Here, by the use of rapid mixing continuous flow electron paramagnetic resonance (EPR), we directly detected the carbonate radical in flow mixtures of peroxynitrite with bicarbonate-carbon dioxide over the pH range of 6-9. The radical was unambiguously identified by its EPR parameters (g = 2.0113; line width = 5.5 G) and by experiments with bicarbonate labeled with ¹³C. In this case, the singlet EPR signal obtained with ¹²C bicarbonate splits into the expected doublet because of ¹³C (a(¹³C)= 11.7 G). The singlet spectrum of the unlabeled radical was invariant between pH 6 and 9, confirming that in this pH range the detected radical is the carbonate radical anion (CO₃). Importantly, in addition to contributing to the understanding of nitrosoperoxocarboxylate decomposition pathways, this is the first report unambiguously demonstrating the formation of the carbonate radical anion at physiological pHs by direct EPR spectroscopy.

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Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

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