JBC Ideal method for primary cell transfection

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Doering, T.
Right arrow Articles by Sandhoff, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Doering, T.
Right arrow Articles by Sandhoff, K.

J Biol Chem, Vol. 274, Issue 16, 11038-11045, April 16, 1999

Sphingolipid Activator Proteins Are Required for Epidermal Permeability Barrier Formation

Thomas DoeringDagger , Walter M. Holleran§, Astrid PotratzDagger , Gabriele VielhaberDagger , Peter M. Elias§, Kunihiko Suzukiparallel , and Konrad SandhoffDagger

From the Dagger  Kekulé-Institut für Organische Chemie und Biochemie, Universität Bonn, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany, the § Department of Dermatology, School of Medicine, University of California and the Dermatology Service and Research Unit (190), Veterans Affairs Medical Center, San Francisco, California 94121, and the parallel  Departments of Neurology and Psychiatry, School of Medicine, University of North Carolina, Chapel Hill, North Carolina, 27599

The epidermal permeability barrier is maintained by extracellular lipid membranes within the interstices of the stratum corneum. Ceramides, the major components of these multilayered membranes, derive in large part from hydrolysis of glucosylceramides mediated by stratum corneum beta -glucocerebrosidase (beta -GlcCerase). Prosaposin (pSAP) is a large precursor protein that is proteolytically cleaved to form four distinct sphingolipid activator proteins, which stimulate enzymatic hydrolysis of sphingolipids, including glucosylceramide. Recently, pSAP has been eliminated in a mouse model using targeted deletion and homologous recombination. In addition to the extracutaneous findings noted previously, our present data indicate that pSAP deficiency in the epidermis has significant consequences including: 1) an accumulation of epidermal glucosylceramides together with below normal levels of ceramides; 2) alterations in lipids that are bound by ester linkages to proteins of the cornified cell envelope; 3) a thickened stratum lucidum with evidence of scaling; and 4) a striking abnormality in lamellar membrane maturation within the interstices of the stratum corneum. Together, these results demonstrate that the production of pSAP, and presumably mature sphingolipid activator protein generation, is required for normal epidermal barrier formation and function. Moreover, detection of significant amounts of covalently bound omega -OH-GlcCer in pSAP-deficient epidermis suggests that deglucosylation to omega -OH-Cer is not a requisite step prior to covalent attachment of lipid to cornified envelope proteins.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
R.-P. Charles, M. Guitard, C. Leyvraz, B. Breiden, M. Haftek, Z. Haftek-Terreau, J.-C. Stehle, K. Sandhoff, and E. Hummler
Postnatal Requirement of the Epithelial Sodium Channel for Maintenance of Epidermal Barrier Function
J. Biol. Chem., February 1, 2008; 283(5): 2622 - 2630.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
N. Epp, G. Furstenberger, K. Muller, S. de Juanes, M. Leitges, I. Hausser, F. Thieme, G. Liebisch, G. Schmitz, and P. Krieg
12R-lipoxygenase deficiency disrupts epidermal barrier function
J. Cell Biol., April 9, 2007; 177(1): 173 - 182.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
V. Vasireddy, Y. Uchida, N. Salem Jr, S. Y. Kim, M. N. A. Mandal, G. B. Reddy, R. Bodepudi, N. L. Alderson, J. C. Brown, H. Hama, et al.
Loss of functional ELOVL4 depletes very long-chain fatty acids (>=C28) and the unique {omega}-O-acylceramides in skin leading to neonatal death
Hum. Mol. Genet., March 1, 2007; 16(5): 471 - 482.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Jennemann, R. Sandhoff, L. Langbein, S. Kaden, U. Rothermel, H. Gallala, K. Sandhoff, H. Wiegandt, and H.-J. Grone
Integrity and Barrier Function of the Epidermis Critically Depend on Glucosylceramide Synthesis
J. Biol. Chem., February 2, 2007; 282(5): 3083 - 3094.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Miyazaki, A. Dobrzyn, P. M. Elias, and J. M. Ntambi
Stearoyl-CoA desaturase-2 gene expression is required for lipid synthesis during early skin and liver development
PNAS, August 30, 2005; 102(35): 12501 - 12506.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
C. Leyvraz, R.-P. Charles, I. Rubera, M. Guitard, S. Rotman, B. Breiden, K. Sandhoff, and E. Hummler
The epidermal barrier function is dependent on the serine protease CAP1/Prss8
J. Cell Biol., August 1, 2005; 170(3): 487 - 496.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Aho, K. Li, Y. Ryoo, C. McGee, A. Ishida-Yamamoto, J. Uitto, and J. F. Klement
Periplakin Gene Targeting Reveals a Constituent of the Cornified Cell Envelope Dispensable for Normal Mouse Development
Mol. Cell. Biol., July 15, 2004; 24(14): 6410 - 6418.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
T. Herrmann, F. van der Hoeven, H.-J. Grone, A. F. Stewart, L. Langbein, I. Kaiser, G. Liebisch, I. Gosch, F. Buchkremer, W. Drobnik, et al.
Mice with targeted disruption of the fatty acid transport protein 4 (Fatp 4, Slc27a4) gene show features of lethal restrictive dermopathy
J. Cell Biol., June 23, 2003; 161(6): 1105 - 1115.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
T. Doering, H. Brade, and K. Sandhoff
Sphingolipid metabolism during epidermal barrier development in mice
J. Lipid Res., October 1, 2002; 43(10): 1727 - 1733.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
G. Vielhaber, L. Brade, B. Lindner, S. Pfeiffer, R. Wepf, U. Hintze, K.-P. Wittern, and H. Brade
Mouse anti-ceramide antiserum: a specific tool for the detection of endogenous ceramide
Glycobiology, June 1, 2001; 11(6): 451 - 457.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
C. G. Schuette, B. Pierstorff, S. Huettler, and K. Sandhoff
Sphingolipid activator proteins: proteins with complex functions in lipid degradation and skin biogenesis
Glycobiology, June 1, 2001; 11(6): 81R - 90R.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
Y. Uchida, M. Hara, H. Nishio, E. Sidransky, S. Inoue, F. Otsuka, A. Suzuki, P. M. Elias, W. M. Holleran, and S. Hamanaka
Epidermal sphingomyelins are precursors for selected stratum corneum ceramides
J. Lipid Res., December 1, 2000; 41(12): 2071 - 2082.
[Abstract] [Full Text]

Home page
 GlycobiologyHome page
L. Brade, G. Vielhaber, E. Heinz, and H. Brade
In vitro characterization of anti-glucosylceramide rabbit antisera
Glycobiology, June 1, 2000; 10(6): 629 - 636.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Nemes, L. N. Marekov, L. Fesus, and P. M. Steinert
A novel function for transglutaminase 1: Attachment of long-chain omega -hydroxyceramides to involucrin by ester bond formation
PNAS, July 20, 1999; 96(15): 8402 - 8407.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.