JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An addition or correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Beall, A.
Right arrow Articles by Brophy, C. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beall, A.
Right arrow Articles by Brophy, C. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 274, Issue 16, 11344-11351, April 16, 1999

The Small Heat Shock-related Protein, HSP20, Is Phosphorylated on Serine 16 during Cyclic Nucleotide-dependent Relaxation

Arthur Beallab, Drew Bagwellbc, David Woodrumabd, Terrence A. Stomingaf, Kanefusa Katog, Atsushi Suzukih, Howard Rasmussenabd, and Colleen M. Brophyabcd

From the Departments of c Surgery, a Medicine (Institute for Molecular Medicine and Genetics), d Cell Biology and Anatomy, and f Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, Georgia 30912, the b Augusta Veterans Administration Medical Center, Augusta, Georgia 30912, the g Department of Biochemistry, Institute for Developmental Research, Human Service Center, Kasugai, Aichi 480-03, Japan, and the h Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236, Japan

The small heat shock-related protein 20 (HSP20) is present in four isoforms in bovine carotid artery smooth muscles. Three of the isoforms are phosphorylated and one is not. Increases in the phosphorylation of two isoforms of HSP20 (isoform 3, pI 5.9; and 8, pI 5.7) are associated with cyclic nucleotide-dependent relaxation of bovine carotid artery smooth muscles. Increases in the phosphorylation of another isoform (isoform 4, pI 6.0) are associated with phorbol ester-induced contraction of bovine carotid artery smooth muscles. In this investigation we determined that isoforms 3 and 8 are phosphorylated on Ser16 of the HSP20 molecule during activation of cAMP-dependent signaling pathways. Phosphorylation state-specific antibodies produced against a peptide containing phosphorylated Ser16 recognized isoforms 3 and 8 but not isoform 4. In human vascular tissue, only isoform 3 is present. Incubation of transiently permeabilized strips of bovine carotid artery smooth muscle with synthetic peptides in which Ser16 is phosphorylated, inhibits contractile responses to high extracellular KCl and to serotonin. These data suggest that phosphorylation of HSP20 on Ser16 modulates cAMP-dependent vasorelaxation.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
S. J. Gunst and W. Zhang
Actin cytoskeletal dynamics in smooth muscle: a new paradigm for the regulation of smooth muscle contraction
Am J Physiol Cell Physiol, September 1, 2008; 295(3): C576 - C587.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
R. B. Penn and J. L. Benovic
Regulation of Heterotrimeric G Protein Signaling in Airway Smooth Muscle
Proceedings of the ATS, January 1, 2008; 5(1): 47 - 57.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. Komalavilas, R. B. Penn, C. R. Flynn, J. Thresher, L. B. Lopes, E. J. Furnish, M. Guo, M. A. Pallero, J. E. Murphy-Ullrich, and C. M. Brophy
The small heat shock-related protein, HSP20, is a cAMP-dependent protein kinase substrate that is involved in airway smooth muscle relaxation
Am J Physiol Lung Cell Mol Physiol, January 1, 2008; 294(1): L69 - L78.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. M. Rembold
Force suppression and the crossbridge cycle in swine carotid artery
Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1003 - C1009.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
B E Cross, H M O'Dea, and D J MacPhee
Expression of small heat shock-related protein 20 (HSP20) in rat myometrium is markedly decreased during late pregnancy and labour
Reproduction, April 1, 2007; 133(4): 807 - 817.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
B. Teng, W. Qin, H. R. Ansari, and S. J. Mustafa
Involvement of p38-mitogen-activated protein kinase in adenosine receptor-mediated relaxation of coronary artery
Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2574 - H2580.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. R. Flynn, C. M. Brophy, E. J. Furnish, P. Komalavilas, D. Tessier, J. Thresher, and L. Joshi
Transduction of phosphorylated heat shock-related protein 20, HSP20, prevents vasospasm of human umbilical artery smooth muscle
J Appl Physiol, May 1, 2005; 98(5): 1836 - 1845.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
X. Chen, H.-Y. Zhang, K. Pavlish, and J. N. Benoit
Effects of chronic portal hypertension on small heat-shock proteins in mesenteric arteries
Am J Physiol Gastrointest Liver Physiol, April 1, 2005; 288(4): G616 - G620.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. K. Meeks, M. L. Ripley, Z. Jin, and C. M. Rembold
Heat shock protein 20-mediated force suppression in forskolin-relaxed swine carotid artery
Am J Physiol Cell Physiol, March 1, 2005; 288(3): C633 - C639.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
G.-C. Fan, G. Chu, B. Mitton, Q. Song, Q. Yuan, and E. G. Kranias
Small Heat-Shock Protein Hsp20 Phosphorylation Inhibits {beta}-Agonist-Induced Cardiac Apoptosis
Circ. Res., June 11, 2004; 94(11): 1474 - 1482.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
C. M. Brophy and P. Komalavilas
Heat Shock-Related Protein 20 (HSP20) in Cardiomyocytes
Circ. Res., May 28, 2004; 94(10): e85 - e85.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Sun, J.-M. Fontaine, J. S. Rest, E. A. Shelden, M. J. Welsh, and R. Benndorf
Interaction of Human HSP22 (HSPB8) with Other Small Heat Shock Proteins
J. Biol. Chem., January 23, 2004; 279(4): 2394 - 2402.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
W. Pipkin, J. A. Johnson, T. L. Creazzo, J. Burch, P. Komalavilas, and C. Brophy
Localization, Macromolecular Associations, and Function of the Small Heat Shock-Related Protein HSP20 in Rat Heart
Circulation, January 28, 2003; 107(3): 469 - 476.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. J. O'Connor and C. M. Rembold
Heat-induced force suppression and HSP20 phosphorylation in swine carotid media
J Appl Physiol, August 1, 2002; 93(2): 484 - 488.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
F. Narberhaus
{alpha}-Crystallin-Type Heat Shock Proteins: Socializing Minichaperones in the Context of a Multichaperone Network
Microbiol. Mol. Biol. Rev., March 1, 2002; 66(1): 64 - 93.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. Brophy, D. Woodrum, and C. M. Rembold
Phosphorylation of HSP20 on Serine 157
J Appl Physiol, February 1, 2002; 92(2): 890 - 891.
[Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. Komalavilas, S. Mehta, C. J. Wingard, D. T. Dransfield, J. Bhalla, J. E. Woodrum, J. R. Molinaro, and C. M. Brophy
PI3-kinase/Akt modulates vascular smooth muscle tone via cAMP signaling pathways
J Appl Physiol, October 1, 2001; 91(4): 1819 - 1827.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. M. Lincoln, N. Dey, and H. Sellak
Signal Transduction in Smooth Muscle: Invited Review: cGMP-dependent protein kinase signaling mechanisms in smooth muscle: from the regulation of tone to gene expression
J Appl Physiol, September 1, 2001; 91(3): 1421 - 1430.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. M. Rembold, M. O'Connor, M. Clarkson, R. L. Wardle, and R. A. Murphy
Signal Transduction in Smooth Muscle: Selected Contribution: HSP20 phosphorylation in nitroglycerin- and forskolin-induced sustained reductions in swine carotid media tone
J Appl Physiol, September 1, 2001; 91(3): 1460 - 1466.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. C. Fuchs, A. D. Giulumian, L. Knoepp, W. Pipkin, M. Dickinson, C. Hayles, and C. Brophy
Stress causes decrease in vascular relaxation linked with altered phosphorylation of heat shock proteins
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2000; 279(2): R492 - R498.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. M. Brophy, L. Knoepp, J. Xin, and J. S. Pollock
Functional expression of NOS 1 in vascular smooth muscle
Am J Physiol Heart Circ Physiol, March 1, 2000; 278(3): H991 - H997.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. A. Woodrum, C. M. Brophy, C. J. Wingard, A. Beall, and H. Rasmussen
Phosphorylation events associated with cyclic nucleotidedependent inhibition of smooth muscle contraction
Am J Physiol Heart Circ Physiol, September 1, 1999; 277(3): H931 - H939.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Butt, D. Immler, H. E. Meyer, A. Kotlyarov, K. Laa{beta}, and M. Gaestel
Heat Shock Protein 27 Is a Substrate of cGMP-dependent Protein Kinase in Intact Human Platelets. PHOSPHORYLATION-INDUCED ACTIN POLYMERIZATION CAUSED BY HSP27 MUTANTS
J. Biol. Chem., March 2, 2001; 276(10): 7108 - 7113.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Benndorf, X. Sun, R. R. Gilmont, K. J. Biederman, M. P. Molloy, C. W. Goodmurphy, H. Cheng, P. C. Andrews, and M. J. Welsh
HSP22, a New Member of the Small Heat Shock Protein Superfamily, Interacts with Mimic of Phosphorylated HSP27 (3DHSP27)
J. Biol. Chem., July 13, 2001; 276(29): 26753 - 26761.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.