| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 17, 11451-11454, April 23, 1999
,,
,,
From the Visual arrestin quenches light-induced
signaling by binding to light-activated, phosphorylated rhodopsin
(P-Rh*). Here we present structure-function data, which in conjunction
with the refined crystal structure of arrestin (Hirsch, J. A.,
Schubert, C., Gurevich, V. V., and Sigler, P. B. (1999)
Cell, in press), support a model for the conversion of a
basal or "inactive" conformation of free arrestin to one that can
bind to and inhibit the light activated receptor. The trigger for this
transition is an interaction of the phosphorylated COOH-terminal
segment of the receptor with arrestin that disrupts intramolecular
interactions, including a hydrogen-bonded network of buried, charged
side chains, referred to as the "polar core." This disruption
permits structural adjustments that allow arrestin to bind to the
receptor. Our mutational survey identifies residues in arrestin
(Arg175, Asp30, Asp296,
Asp303, Arg382), which when altered bypass the
need for the interaction with the receptor's phosphopeptide, enabling
arrestin to bind to activated, nonphosphorylated rhodopsin (Rh*). These
mutational changes disrupt interactions and substructures which the
crystallographic model and previous biochemical studies have shown are
responsible for maintaining the inactive state. The molecular basis for
these disruptions was confirmed by successfully introducing
structure-based second site substitutions that restored the critical
interactions. The nearly absolute conservation of the mutagenically
sensitive residues throughout the arrestin family suggests that this
mechanism is likely to be applicable to arrestin-mediated
desensitization of most G-protein-coupled receptors.
Ralph and Muriel Roberts Laboratory for
Vision Science, Sun Health Research Institute, Sun City, Arizona 85372 and the § Howard Hughes Medical Institute, Department
of Molecular Biophysics and Biochemistry, Yale University, New
Haven, Connecticut 06510
This article has been cited by other articles:
|
|
 |
|
  S. A. Vishnivetskiy, D. Raman, J. Wei, M. J. Kennedy, J. B. Hurley, and V. V. Gurevich Regulation of Arrestin Binding by Rhodopsin Phosphorylation Level J. Biol. Chem., November 2, 2007; 282(44): 32075 - 32083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. N. Nobles, Z. Guan, K. Xiao, T. G. Oas, and R. J. Lefkowitz The Active Conformation of beta-Arrestin1: DIRECT EVIDENCE FOR THE PHOSPHATE SENSOR IN THE N-DOMAIN AND CONFORMATIONAL DIFFERENCES IN THE ACTIVE STATES OF beta-ARRESTINS1 AND -2 J. Biol. Chem., July 20, 2007; 282(29): 21370 - 21381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Hanson, D. J. Francis, S. A. Vishnivetskiy, C. S. Klug, and V. V. Gurevich Visual Arrestin Binding to Microtubules Involves a Distinct Conformational Change J. Biol. Chem., April 7, 2006; 281(14): 9765 - 9772. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Hanson, D. J. Francis, S. A. Vishnivetskiy, E. A. Kolobova, W. L. Hubbell, C. S. Klug, and V. V. Gurevich Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin PNAS, March 28, 2006; 103(13): 4900 - 4905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Hanson and V. V. Gurevich The Differential Engagement of Arrestin Surface Charges by the Various Functional Forms of the Receptor J. Biol. Chem., February 10, 2006; 281(6): 3458 - 3462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Z. Szabo, M. Numata, V. Lukashova, P. Iannuzzi, and J. Orlowski {beta}-Arrestins bind and decrease cell-surface abundance of the Na+/H+ exchanger NHE5 isoform PNAS, February 22, 2005; 102(8): 2790 - 2795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Nair, S. M. Hanson, M. J. Kennedy, J. B. Hurley, V. V. Gurevich, and V. Z. Slepak Direct Binding of Visual Arrestin to Microtubules Determines the Differential Subcellular Localization of Its Splice Variants in Rod Photoreceptors J. Biol. Chem., September 24, 2004; 279(39): 41240 - 41248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ling, M. Ascano, P. Robinson, and S. K. Gregurick Experimental and Computational Studies of the Desensitization Process in the Bovine Rhodopsin-Arrestin Complex Biophys. J., April 1, 2004; 86(4): 2445 - 2454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O.-J. Kim, B. R. Gardner, D. B. Williams, P. S. Marinec, D. M. Cabrera, J. D. Peters, C. C. Mak, K.-M. Kim, and D. R. Sibley The Role of Phosphorylation in D1 Dopamine Receptor Desensitization: EVIDENCE FOR A NOVEL MECHANISM OF ARRESTIN ASSOCIATION J. Biol. Chem., February 27, 2004; 279(9): 7999 - 8010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Vishnivetskiy, M. M. Hosey, J. L. Benovic, and V. V. Gurevich Mapping the Arrestin-Receptor Interface: STRUCTURAL ELEMENTS RESPONSIBLE FOR RECEPTOR SPECIFICITY OF ARRESTIN PROTEINS J. Biol. Chem., January 9, 2004; 279(2): 1262 - 1268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhang, N. Cuenca, T. Ivanova, J. Church-Kopish, J. M. Frederick, P. R. MacLeish, and W. Baehr Identification and Light-Dependent Translocation of a Cone-Specific Antigen, Cone Arrestin, Recognized by Monoclonal Antibody 7G6 Invest. Ophthalmol. Vis. Sci., July 1, 2003; 44(7): 2858 - 2867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Gray, A. Bhatnagar, V. V. Gurevich, and B. L. Roth The Interaction of a Constitutively Active Arrestin with the Arrestin-Insensitive 5-HT2A Receptor Induces Agonist-Independent Internalization Mol. Pharmacol., May 1, 2003; 63(5): 961 - 972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Pan, E. V. Gurevich, and V. V. Gurevich The Nature of the Arrestin{middle dot}Receptor Complex Determines the Ultimate Fate of the Internalized Receptor J. Biol. Chem., March 21, 2003; 278(13): 11623 - 11632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Key, T. D. Foutz, V. V. Gurevich, L. A. Sklar, and E. R. Prossnitz N-Formyl Peptide Receptor Phosphorylation Domains Differentially Regulate Arrestin and Agonist Affinity J. Biol. Chem., January 31, 2003; 278(6): 4041 - 4047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-H. Kim, J.-G. Kang, S.-S. Yang, K.-S. Chung, P.-S. Song, and C.-M. Park A Phytochrome-Associated Protein Phosphatase 2A Modulates Light Signals in Flowering Time Control in Arabidopsis PLANT CELL, December 1, 2002; 14(12): 3043 - 3056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Vishnivetskiy, J. A. Hirsch, M.-G. Velez, Y. V. Gurevich, and V. V. Gurevich Transition of Arrestin into the Active Receptor-binding State Requires an Extended Interdomain Hinge J. Biol. Chem., November 8, 2002; 277(46): 43961 - 43967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Schroder, A. Pulvermuller, and K. P. Hofmann Arrestin and Its Splice Variant Arr1-370A (p44). MECHANISM AND BIOLOGICAL ROLE OF THEIR INTERACTION WITH RHODOPSIN J. Biol. Chem., November 8, 2002; 277(46): 43987 - 43996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Huttenrauch, A. Nitzki, F.-T. Lin, S. Honing, and M. Oppermann beta -Arrestin Binding to CC Chemokine Receptor 5 Requires Multiple C-terminal Receptor Phosphorylation Sites and Involves a Conserved Asp-Arg-Tyr Sequence Motif J. Biol. Chem., August 16, 2002; 277(34): 30769 - 30777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kishi, H. Krishnamurthy, C. Galet, R. S. Bhaskaran, and M. Ascoli Identification of a Short Linear Sequence Present in the C-terminal Tail of the Rat Follitropin Receptor That Modulates Arrestin-3 Binding in a Phosphorylation-independent Fashion J. Biol. Chem., June 7, 2002; 277(24): 21939 - 21946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mukherjee, V. V. Gurevich, A. Preninger, H. E. Hamm, M.-F. Bader, A. T. Fazleabas, L. Birnbaumer, and M. Hunzicker-Dunn Aspartic Acid 564 in the Third Cytoplasmic Loop of the Luteinizing Hormone/Choriogonadotropin Receptor Is Crucial for Phosphorylation-independent Interaction with Arrestin2 J. Biol. Chem., May 10, 2002; 277(20): 17916 - 17927. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Mundell, A.-L. Matharu, G. Pula, D. Holman, P. J. Roberts, and E. Kelly Metabotropic Glutamate Receptor 1 Internalization Induced by Muscarinic Acetylcholine Receptor Activation: Differential Dependency of Internalization of Splice Variants on Nonvisual Arrestins Mol. Pharmacol., May 1, 2002; 61(5): 1114 - 1123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Dinculescu, J. H. McDowell, S. A. Amici, D. R. Dugger, N. Richards, P. A. Hargrave, and W. C. Smith Insertional Mutagenesis and Immunochemical Analysis of Visual Arrestin Interaction with Rhodopsin J. Biol. Chem., March 29, 2002; 277(14): 11703 - 11708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Potter, T. A. Key, V. V. Gurevich, L. A. Sklar, and E. R. Prossnitz Arrestin Variants Display Differential Binding Characteristics for the Phosphorylated N-Formyl Peptide Receptor Carboxyl Terminus J. Biol. Chem., March 8, 2002; 277(11): 8970 - 8978. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Vilardaga, C. Krasel, S. Chauvin, T. Bambino, M. J. Lohse, and R. A. Nissenson Internalization Determinants of the Parathyroid Hormone Receptor Differentially Regulate beta -Arrestin/Receptor Association J. Biol. Chem., March 1, 2002; 277(10): 8121 - 8129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Qian, L. Pipolo, and W. G. Thomas Association of {beta}-Arrestin 1 with the Type 1A Angiotensin II Receptor Involves Phosphorylation of the Receptor Carboxyl Terminus and Correlates with Receptor Internalization Mol. Endocrinol., October 1, 2001; 15(10): 1706 - 1719. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. G. Ferguson Evolving Concepts in G Protein-Coupled Receptor Endocytosis: The Role in Receptor Desensitization and Signaling Pharmacol. Rev., March 1, 2001; 53(1): 1 - 24. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Seibold, B. Williams, Z.-F. Huang, J. Friedman, R. H. Moore, B. J. Knoll, and R. B. Clark Localization of the Sites Mediating Desensitization of the beta 2-Adrenergic Receptor by the GRK Pathway Mol. Pharmacol., November 1, 2000; 58(5): 1162 - 1173. [Abstract] [Full Text]
|
|
|
|
|
|
W. C. Smith, E. V. Gurevich, D. R. Dugger, S. A. Vishnivetskiy, C. L. Shelamer, J. H. McDowell, and V. V. Gurevich Cloning and Functional Characterization of Salamander Rod and Cone Arrestins Invest. Ophthalmol. Vis. Sci., August 1, 2000; 41(9): 2445 - 2455. [Abstract] [Full Text]
|
|
|
|
|
|
C. Lee, S.-H. Park, M.-Y. Lee, and M.-H. Yu Regulation of protein function by native metastability PNAS, July 5, 2000; 97(14): 7727 - 7731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Itokawa, Z. Lin, N.-S. Cai, C. Wu, S. Kitayama, J.-B. Wang, and G. R. Uhl Dopamine Transporter Transmembrane Domain Polar Mutants: Delta G and Delta Delta G Values Implicate Regions Important for Transporter Functions Mol. Pharmacol., June 1, 2000; 57(6): 1093 - 1103. [Abstract] [Full Text]
|
|
|
|
|
|
K. B. Lee, J. A. Ptasienski, R. Pals-Rylaarsdam, V. V. Gurevich, and M. M. Hosey Arrestin Binding to the M2 Muscarinic Acetylcholine Receptor Is Precluded by an Inhibitory Element in the Third Intracellular Loop of the Receptor J. Biol. Chem., March 24, 2000; 275(13): 9284 - 9289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakamura, X. Liu, and M. Ascoli Seven Non-contiguous Intracellular Residues of the Lutropin/Choriogonadotropin Receptor Dictate the Rate of Agonist-induced Internalization and Its Sensitivity to Non-visual Arrestins J. Biol. Chem., January 7, 2000; 275(1): 241 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Bennett, D. C. Maestas, and E. R. Prossnitz Arrestin Binding to the G Protein-coupled N-Formyl Peptide Receptor Is Regulated by the Conserved "DRY" Sequence J. Biol. Chem., August 4, 2000; 275(32): 24590 - 24594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. B. Lee, J. A. Ptasienski, M. Bunemann, and M. M. Hosey Acidic Amino Acids Flanking Phosphorylation Sites in the M2 Muscarinic Receptor Regulate Receptor Phosphorylation, Internalization, and Interaction with Arrestins J. Biol. Chem., November 10, 2000; 275(46): 35767 - 35777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pulvermuller, K. Schroder, T. Fischer, and K. P. Hofmann Interactions of Metarhodopsin II. ARRESTIN PEPTIDES COMPETE WITH ARRESTIN AND TRANSDUCIN J. Biol. Chem., November 22, 2000; 275(48): 37679 - 37685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Vishnivetskiy, C. Schubert, G. C. Climaco, Y. V. Gurevich, M.-G. Velez, and V. V. Gurevich An Additional Phosphate-binding Element in Arrestin Molecule. IMPLICATIONS FOR THE MECHANISM OF ARRESTIN ACTIVATION J. Biol. Chem., December 22, 2000; 275(52): 41049 - 41057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Celver, J. Lowe, A. Kovoor, V. V. Gurevich, and C. Chavkin Threonine 180 Is Required for G-protein-coupled Receptor Kinase 3- and beta -Arrestin 2-mediated Desensitization of the {micro}-Opioid Receptor in Xenopus Oocytes J. Biol. Chem., February 9, 2001; 276(7): 4894 - 4900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Vilardaga, M. Frank, C. Krasel, C. Dees, R. A. Nissenson, and M. J. Lohse Differential Conformational Requirements for Activation of G Proteins and the Regulatory Proteins Arrestin and G Protein-coupled Receptor Kinase in the G Protein-coupled Receptor for Parathyroid Hormone (PTH)/PTH-related Protein J. Biol. Chem., August 31, 2001; 276(36): 33435 - 33443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Celver, S. A. Vishnivetskiy, C. Chavkin, and V. V. Gurevich Conservation of the Phosphate-sensitive Elements in the Arrestin Family of Proteins J. Biol. Chem., March 8, 2002; 277(11): 9043 - 9048. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Key, T. A. Bennett, T. D. Foutz, V. V. Gurevich, L. A. Sklar, and E. R. Prossnitz Regulation of Formyl Peptide Receptor Agonist Affinity by Reconstitution with Arrestins and Heterotrimeric G Proteins J. Biol. Chem., December 21, 2001; 276(52): 49204 - 49212. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
