| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 17, 11593-11598, April 23, 1999
From the Cancer Research Center, The Burnham Institute,
La Jolla, California 92037
In vivo phage display is a powerful
method to study organ- and tissue-specific vascular addresses. Using
this approach, peptides capable of tissue-specific homing can be
identified by performing a selection for that trait in
vivo. We recently showed that the CGFECVRQCPERC (termed GFE-1)
peptide can selectively bind to mouse lung vasculature after an
intravenous injection. Our aim in the present study was to identify the
receptor for this lung-homing peptide. By using affinity
chromatography, we isolated a 55-kDa lung cell-surface protein that
selectively binds to the GFE-1 peptide. Protein sequencing established
the identity of the receptor as membrane dipeptidase (MDP), a
cell-surface zinc metalloprotease involved in the metabolism of
glutathione, leukotriene D4, and certain 
-lactam
antibiotics. Phage particles displaying the GFE-1 peptide selectively
bind to COS-1 cells transfected with the murine MDP cDNA. Moreover,
the synthetic GFE-1 peptide could inhibit MDP activity. By establishing
MDP as the receptor for the GFE-1 peptide, our results suggest
potential applications for both MDP and the GFE-1 peptide in delivery
of compounds to the lungs. This work also demonstrates that
cell-surface proteases can be involved in tissue-specific homing.
This article has been cited by other articles:
|
|
 |
|
  R. Robert, M.-J. Jacobin-Valat, D. Daret, S. Miraux, A. T. Nurden, J.-M. Franconi, and G. Clofent-Sanchez Identification of Human scFvs Targeting Atherosclerotic Lesions: SELECTION BY SINGLE ROUND IN VIVO PHAGE DISPLAY J. Biol. Chem., December 29, 2006; 281(52): 40135 - 40143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Li, R. Zhao, X. Wu, Y. Sun, M. Yao, J. Li, Y. Xu, and J. Gu Identification and characterization of a novel peptide ligand of epidermal growth factor receptor for targeted delivery of therapeutics FASEB J, December 1, 2005; 19(14): 1978 - 1985. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Oh, I. Mohiuddin, Y. Sun, J. B. Putnam Jr, W. K. Hong, W. Arap, and R. Pasqualini Phenotypic Diversity of the Lung Vasculature in Experimental Models of Metastases Chest, December 1, 2005; 128(6_suppl): 596S - 600S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Oh, I. Mohiuddin, Y. Sun, J. B. Putnam Jr, W. K. Hong, W. Arap, and R. Pasqualini Phenotypic Diversity of the Lung Vasculature in Experimental Models of Metastases Chest, December 1, 2005; 128(6_suppl): 596S - 600S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhang, J. A. Hoffman, and E. Ruoslahti Molecular Profiling of Heart Endothelial Cells Circulation, September 13, 2005; 112(11): 1601 - 1611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. J. Yao, M. G. Ozawa, M. Trepel, W. Arap, D. M. McDonald, and R. Pasqualini Targeting Pancreatic Islets with Phage Display Assisted by Laser Pressure Catapult Microdissection Am. J. Pathol., February 1, 2005; 166(2): 625 - 636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-S. Ding, Y.-J. Zhou, X.-Y. Chen, J. Zhang, P.-X. Zhang, Z.-Y. Sun, X.-Y. Tan, and J.-N. Liu Lung Endothelium Targeting for Pulmonary Embolism Thrombolysis Circulation, December 9, 2003; 108(23): 2892 - 2898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-C. Wu, M.-Y. Jung, C.-Y. Chiu, T.-T. Chao, S.-C. Lai, J.-T. Jan, and M.-F. Shaio Identification of a dengue virus type 2 (DEN-2) serotype-specific B-cell epitope and detection of DEN-2-immunized animal serum samples using an epitope-based peptide antigen J. Gen. Virol., October 1, 2003; 84(10): 2771 - 2779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Benson, E. B. Gottlin, D. J. Christensen, and P. T. Hamilton Intracellular Expression of Peptide Fusions for Demonstration of Protein Essentiality in Bacteria Antimicrob. Agents Chemother., September 1, 2003; 47(9): 2875 - 2881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-C. Cheng, M. Abdel-Ghany, and B. U. Pauli A Novel Consensus Motif in Fibronectin Mediates Dipeptidyl Peptidase IV Adhesion and Metastasis J. Biol. Chem., June 27, 2003; 278(27): 24600 - 24607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Koolpe, M. Dail, and E. B. Pasquale An Ephrin Mimetic Peptide That Selectively Targets the EphA2 Receptor J. Biol. Chem., November 27, 2002; 277(49): 46974 - 46979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Akerman, W. C. W. Chan, P. Laakkonen, S. N. Bhatia, and E. Ruoslahti Nanocrystal targeting in vivo PNAS, October 1, 2002; 99(20): 12617 - 12621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Kolonin, R. Pasqualini, and W. Arap Teratogenicity induced by targeting a placental immunoglobulin transporter PNAS, October 1, 2002; 99(20): 13055 - 13060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Essler and E. Ruoslahti Molecular specialization of breast vasculature: A breast-homing phage-displayed peptide binds to aminopeptidase P in breast vasculature PNAS, February 19, 2002; 99(4): 2252 - 2257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Arap, W. Haedicke, M. Bernasconi, R. Kain, D. Rajotte, S. Krajewski, H. M. Ellerby, D. E. Bredesen, R. Pasqualini, and E. Ruoslahti Targeting the prostate for destruction through a vascular address PNAS, February 5, 2002; 99(3): 1527 - 1531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-C. Wu, C.-T. Yeh, Y.-L. Huang, L.-J. Tarn, and C.-C. Lung Characterization of Neutralizing Antibodies and Identification of Neutralizing Epitope Mimics on the Clostridium botulinum Neurotoxin Type A Appl. Envir. Microbiol., July 1, 2001; 67(7): 3201 - 3207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. White, S. A. Nicklin, T. Sawamura, and A. H. Baker Identification of Peptides That Target the Endothelial Cell-Specific LOX-1 Receptor Hypertension, February 1, 2001; 37(2): 449 - 455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Nicklin, S. J. White, S. J. Watkins, R. E. Hawkins, and A. H. Baker Selective Targeting of Gene Transfer to Vascular Endothelial Cells by Use of Peptides Isolated by Phage Display Circulation, July 11, 2000; 102(2): 231 - 237. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
