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J Biol Chem, Vol. 274, Issue 18, 12201-12204, April 30, 1999

COMMUNICATION
Base Excision Repair of N-Methylpurines in a Yeast Minichromosome
EFFECTS OF TRANSCRIPTION, DNA SEQUENCE, AND NUCLEOSOME POSITIONING

From the Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington 99164-4660

Base excision repair of dimethyl sulfate induced N-methylpurines (NMPs) was measured in a yeast minichromosome that has a galactose-inducible GAL1:URA3 fusion gene, a constitutively expressed HIS3 gene, and varied regions of chromatin structure. Removal rates of NMPs varied dramatically (>20-fold) at different sites along three selected fragments encompassing a total length of 1775 base pairs. Repair of NMPs was not coupled to transcription, because the transcribed strands of HIS3 and induced GAL1:URA3 were not repaired faster than the nontranscribed strands. However, the repair rate of NMPs was significantly affected by the nearest neighbor nucleotides. Slow repair occurred at NMPs between purines, especially guanines, whereas fast repair occurred at NMPs between pyrimidines. NMPs between a purine and pyrimidine were repaired at moderate rates. Moreover, a rough correlation between nucleosome positions and repair rates exists in some but not all regions that were analyzed.

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