|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 18, 12217-12221, April 30, 1999
From the Department of Biological Chemistry, The Weizmann Institute
of Science, Rehovot, Israel 76100 and the Trans-splicing in trypanosomes
involves the addition of a common spliced leader (SL) sequence, which
is derived from a small RNA, the SL RNA, to all mRNA precursors.
The SL RNA is present in the cell in the form of a ribonucleoprotein,
the SL RNP. Using conventional chromatography and affinity selection
with 2'-O-methylated RNA oligonucleotides at high ionic
strength, five proteins of 70, 16, 13, 12, and 8 kDa were co-selected
with the SL RNA from Leptomonas collosoma, representing the
SL RNP core particle. Under conditions of lower ionic strength,
additional proteins of 28 and 20 kDa were revealed. On the basis of
peptide sequences, the gene coding for a protein with a predicted
molecular weight of 11.9 kDa was cloned and identified as homologue of
the cis-spliceosomal SmE. The protein carries the Sm motifs
1 and 2 characteristic of Sm antigens that bind to all known
cis-spliceosomal uridylic acid-rich small nuclear RNAs (U
snRNAs), suggesting the existence of Sm proteins in trypanosomes. This
finding is of special interest because trypanosome snRNPs are the only
snRNPs examined to date that are not recognized by anti-Sm antibodies.
Because of the early divergence of trypanosomes from the eukaryotic
lineage, the trypanosome SmE protein represents one of the primordial
Sm proteins in nature.
Purification of the Spliced Leader Ribonucleoprotein Particle
from Leptomonas collosoma Revealed the Existence of an Sm
Protein in Trypanosomes
CLONING THE SmE HOMOLOGUE
,, and Institut
für Biochemie, Humboldt-Universität/Charité
Monbijou-Strasse 2a, D-10117 Berlin, Germany
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  I. D. Tkacz, Y. Lustig, M. Z. Stern, M. Biton, M. Salmon-Divon, A. Das, V. Bellofatto, and S. Michaeli Identification of novel snRNA-specific Sm proteins that bind selectively to U2 and U4 snRNAs in Trypanosoma brucei RNA, January 1, 2007; 13(1): 30 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Dumas, C. Chow, M. Muller, and B. Papadopoulou A Novel Class of Developmentally Regulated Noncoding RNAs in Leishmania Eukaryot. Cell, December 1, 2006; 5(12): 2033 - 2046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Liu, X.-h. Liang, S. Uliel, M. Belahcen, R. Unger, and S. Michaeli Identification and Functional Characterization of Lsm Proteins in Trypanosoma brucei J. Biol. Chem., April 30, 2004; 279(18): 18210 - 18219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mandelboim, S. Barth, M. Biton, X.-h. Liang, and S. Michaeli Silencing of Sm Proteins in Trypanosoma brucei by RNA Interference Captured a Novel Cytoplasmic Intermediate in Spliced Leader RNA Biogenesis J. Biol. Chem., December 19, 2003; 278(51): 51469 - 51478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-h. Liang, A. Haritan, S. Uliel, and S. Michaeli trans and cis Splicing in Trypanosomatids: Mechanism, Factors, and Regulation Eukaryot. Cell, October 1, 2003; 2(5): 830 - 840. [Full Text] [PDF]
|
|
|
|
|
|
G. M. Zeiner, N. R. Sturm, and D. A. Campbell Exportin 1 Mediates Nuclear Export of the Kinetoplastid Spliced Leader RNA Eukaryot. Cell, April 1, 2003; 2(2): 222 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mandelboim, C. L. Estrano, C. Tschudi, E. Ullu, and S. Michaeli On the Role of Exon and Intron Sequences in trans-Splicing Utilization and cap 4 Modification of the Trypanosomatid Leptomonas collosoma SL RNA J. Biol. Chem., September 13, 2002; 277(38): 35210 - 35218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Evans, I. Perez, M. MacMorris, D. Leake, C. J. Wilusz, and T. Blumenthal A complex containing CstF-64 and the SL2 snRNP connects mRNA 3' end formation and trans-splicing in C. elegans operons Genes & Dev., October 1, 2001; 15(19): 2562 - 2571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Evans and T. Blumenthal trans Splicing of Polycistronic Caenorhabditis elegans Pre-mRNAs: Analysis of the SL2 RNA Mol. Cell. Biol., September 15, 2000; 20(18): 6659 - 6667. [Abstract] [Full Text]
|
|
|
|
 |
|
 Z. Palfi, S. Lücke, H.-W. Lahm, W. S. Lane, V. Kruft, E. Bragado-Nilsson, B. Séraphin, and A. Bindereif The spliceosomal snRNP core complex of Trypanosoma brucei: Cloning and functional analysis reveals seven Sm protein constituents PNAS, July 12, 2000; (2000) 150236097. [Abstract] [Full Text]
|
|
|
|
|
|
L. Li, L. R. Otake, Y.-x. Xu, and S. Michaeli The trans-Spliceosomal U4 RNA from the Monogenetic Trypanosomatid Leptomonas collosoma. CLONING AND IDENTIFICATION OF A TRANSCRIBED tRNA-LIKE ELEMENT THAT CONTROLS ITS EXPRESSION J. Biol. Chem., January 28, 2000; 275(4): 2259 - 2264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-x. Xu, L. Liu, and S. Michaeli Functional Analyses of Positions across the 5' Splice Site of the Trypanosomatid Spliced Leader RNA. IMPLICATIONS FOR BASE-PAIR INTERACTION WITH U5 AND U6 snRNAs J. Biol. Chem., September 1, 2000; 275(36): 27883 - 27892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-x. Xu, L. Liu, C. Lopez-Estrano, and S. Michaeli Expression Studies on Clustered Trypanosomatid Box C/D Small Nucleolar RNAs J. Biol. Chem., April 20, 2001; 276(17): 14289 - 14298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Palfi, S. Lucke, H.-W. Lahm, W. S. Lane, V. Kruft, E. Bragado-Nilsson, B. Seraphin, and A. Bindereif The spliceosomal snRNP core complex of Trypanosoma brucei: Cloning and functional analysis reveals seven Sm protein constituents PNAS, August 1, 2000; 97(16): 8967 - 8972. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |