HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hayashi, H. Articles by Nishimura, M. Search for Related Content PubMed PubMed Citation Articles by Hayashi, H. Articles by Nishimura, M. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 18, 12715-12721, April 30, 1999

A Novel Acyl-CoA Oxidase That Can Oxidize Short-chain Acyl-CoA in Plant Peroxisomes

Hiroshi Hayashi^§, Luigi De Bellis^¶, Adriana Ciurli^¶, Maki Kondo, Makoto Hayashi^§, and Mikio Nishimura^§

From the  Department of Cell Biology, National Institute for Basic Biology and the ^§ Department of Molecular Biomechanics, Graduate University for Advanced Studies, Okazaki 444-8585, Japan and the ^¶ Dipartimento di Biologia delle Piante Agrarie, via Mariscoglio 34, 56124 Pisa, Italy

Short-chain acyl-CoA oxidases are -oxidation enzymes that are active on short-chain acyl-CoAs and that appear to be present in higher plant peroxisomes and absent in mammalian peroxisomes. Therefore, plant peroxisomes are capable of performing complete -oxidation of acyl-CoA chains, whereas mammalian peroxisomes can perform -oxidation of only those acyl-CoA chains that are larger than octanoyl-CoA (C₈). In this report, we have shown that a novel acyl-CoA oxidase can oxidize short-chain acyl-CoA in plant peroxisomes. A peroxisomal short-chain acyl-CoA oxidase from Arabidopsis was purified following the expression of the Arabidopsis cDNA in a baculovirus expression system. The purified enzyme was active on butyryl-CoA (C₄), hexanoyl-CoA (C₆), and octanoyl-CoA (C₈). Cell fractionation and immunocytochemical analysis revealed that the short-chain acyl-CoA oxidase is localized in peroxisomes. The expression pattern of the short-chain acyl-CoA oxidase was similar to that of peroxisomal 3-ketoacyl-CoA thiolase, a marker enzyme of fatty acid -oxidation, during post-germinative growth. Although the molecular structure and amino acid sequence of the enzyme are similar to those of mammalian mitochondrial acyl-CoA dehydrogenase, the purified enzyme has no activity as acyl-CoA dehydrogenase. These results indicate that the short-chain acyl-CoA oxidases function in fatty acid -oxidation in plant peroxisomes, and that by the cooperative action of long- and short-chain acyl-CoA oxidases, plant peroxisomes are capable of performing the complete -oxidation of acyl-CoA.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				Y. Arai, M. Hayashi, and M. Nishimura Proteomic Identification and Characterization of a Novel Peroxisomal Adenine Nucleotide Transporter Supplying ATP for Fatty Acid {beta}-Oxidation in Soybean and Arabidopsis PLANT CELL, December 1, 2008; 20(12): 3227 - 3240. [Abstract] [Full Text] [PDF]

				B. S. Zheng, E. Ronnberg, L. Viitanen, T. A. Salminen, K. Lundgren, T. Moritz, and J. Edqvist Arabidopsis sterol carrier protein-2 is required for normal development of seeds and seedlings J. Exp. Bot., September 1, 2008; 59(12): 3485 - 3499. [Abstract] [Full Text] [PDF]

				B. K. Zolman, N. Martinez, A. Millius, A. R. Adham, and B. Bartel Identification and Characterization of Arabidopsis Indole-3-Butyric Acid Response Mutants Defective in Novel Peroxisomal Enzymes Genetics, September 1, 2008; 180(1): 237 - 251. [Abstract] [Full Text] [PDF]

				Y. Arai, M. Hayashi, and M. Nishimura Proteomic Analysis of Highly Purified Peroxisomes from Etiolated Soybean Cotyledons Plant Cell Physiol., April 1, 2008; 49(4): 526 - 539. [Abstract] [Full Text] [PDF]

				K. A. Lucas, J. R. Filley, J. M. Erb, E. R. Graybill, and J. W. Hawes Peroxisomal Metabolism of Propionic Acid and Isobutyric Acid in Plants J. Biol. Chem., August 24, 2007; 282(34): 24980 - 24989. [Abstract] [Full Text] [PDF]

				J. Mackenzie, L. Pedersen, S. Arent, and A. Henriksen Controlling Electron Transfer in Acyl-CoA Oxidases and Dehydrogenases: A STRUCTURAL VIEW J. Biol. Chem., October 13, 2006; 281(41): 31012 - 31020. [Abstract] [Full Text] [PDF]

				K. Ishizaki, T. R. Larson, N. Schauer, A. R. Fernie, I. A. Graham, and C. J. Leaver The Critical Role of Arabidopsis Electron-Transfer Flavoprotein:Ubiquinone Oxidoreductase during Dark-Induced Starvation PLANT CELL, September 1, 2005; 17(9): 2587 - 2600. [Abstract] [Full Text] [PDF]

				S. Reumann, C. Ma, S. Lemke, and L. Babujee AraPerox. A Database of Putative Arabidopsis Proteins from Plant Peroxisomes Plant Physiology, September 1, 2004; 136(1): 2587 - 2608. [Abstract] [Full Text] [PDF]

				S. Reumann Specification of the Peroxisome Targeting Signals Type 1 and Type 2 of Plant Peroxisomes by Bioinformatics Analyses Plant Physiology, June 1, 2004; 135(2): 783 - 800. [Abstract] [Full Text] [PDF]

				M. C. Castillo, C. Martinez, A. Buchala, J.-P. Metraux, and J. Leon Gene-Specific Involvement of {beta}-Oxidation in Wound-Activated Responses in Arabidopsis Plant Physiology, May 1, 2004; 135(1): 85 - 94. [Abstract] [Full Text] [PDF]

				T. Kamada, K. Nito, H. Hayashi, S. Mano, M. Hayashi, and M. Nishimura Functional Differentiation of Peroxisomes Revealed by Expression Profiles of Peroxisomal Genes in Arabidopsis thaliana Plant Cell Physiol., December 15, 2003; 44(12): 1275 - 1289. [Abstract] [Full Text] [PDF]

				Y. Fukao, M. Hayashi, I. Hara-Nishimura, and M. Nishimura Novel Glyoxysomal Protein Kinase, GPK1, Identified by Proteomic Analysis of Glyoxysomes in Etiolated Cotyledons of Arabidopsis thaliana Plant Cell Physiol., October 15, 2003; 44(10): 1002 - 1012. [Abstract] [Full Text] [PDF]

				E. L. Rylott, C. A. Rogers, A. D. Gilday, T. Edgell, T. R. Larson, and I. A Graham Arabidopsis Mutants in Short- and Medium-chain Acyl-CoA Oxidase Activities Accumulate Acyl-CoAs and Reveal That Fatty Acid {beta}-Oxidation Is Essential for Embryo Development J. Biol. Chem., June 6, 2003; 278(24): 21370 - 21377. [Abstract] [Full Text] [PDF]

				H. Hayashi, L. De Bellis, Y. Hayashi, K. Nito, A. Kato, M. Hayashi, I. Hara-Nishimura, and M. Nishimura Molecular Characterization of an Arabidopsis Acyl-Coenzyme A Synthetase Localized on Glyoxysomal Membranes Plant Physiology, December 1, 2002; 130(4): 2019 - 2026. [Abstract] [Full Text] [PDF]

				S. Lawand, A.-J. Dorne, D. Long, G. Coupland, R. Mache, and P. Carol Arabidopsis A BOUT DE SOUFFLE, Which Is Homologous with Mammalian Carnitine Acyl Carrier, Is Required for Postembryonic Growth in the Light PLANT CELL, September 1, 2002; 14(9): 2161 - 2173. [Abstract] [Full Text] [PDF]

				Y. Arai, H. Nakashita, Y. Suzuki, Y. Kobayashi, T. Shimizu, M. Yasuda, Y. Doi, and I. Yamaguchi Synthesis of a Novel Class of Polyhydroxyalkanoates in Arabidopsis Peroxisomes, and Their Use in Monitoring Short-Chain-Length Intermediates of {beta}-Oxidation Plant Cell Physiol., May 15, 2002; 43(5): 555 - 562. [Abstract] [Full Text] [PDF]

				K. Nito, M. Hayashi, and M. Nishimura Direct Interaction and Determination of Binding Domains among Peroxisomal Import Factors in Arabidopsis thaliana Plant Cell Physiol., April 15, 2002; 43(4): 355 - 366. [Abstract] [Full Text] [PDF]

				S. Mano, C. Nakamori, M. Hayashi, A. Kato, M. Kondo, and M. Nishimura Distribution and Characterization of Peroxisomes in Arabidopsis by Visualization with GFP: Dynamic Morphology and Actin-Dependent Movement Plant Cell Physiol., March 1, 2002; 43(3): 331 - 341. [Abstract] [Full Text] [PDF]

				E. Watanabe, T. Shimada, M. Kuroyanagi, M. Nishimura, and I. Hara-Nishimura Calcium-mediated Association of a Putative Vacuolar Sorting Receptor PV72 with a Propeptide of 2S Albumin J. Biol. Chem., March 1, 2002; 277(10): 8708 - 8715. [Abstract] [Full Text] [PDF]

				M. Kuroyanagi, M. Nishimura, and I. Hara-Nishimura Activation of Arabidopsis Vacuolar Processing Enzyme by Self-Catalytic Removal of an Auto-Inhibitory Domain of the C-Terminal Propeptide Plant Cell Physiol., February 1, 2002; 43(2): 143 - 151. [Abstract] [Full Text] [PDF]

				M. Hayashi, K. Nito, R. Takei-Hoshi, M. Yagi, M. Kondo, A. Suenaga, T. Yamaya, and M. Nishimura Ped3p is a Peroxisomal ATP-Binding Cassette Transporter that might Supply Substrates for Fatty Acid {beta}-Oxidation Plant Cell Physiol., January 1, 2002; 43(1): 1 - 11. [Abstract] [Full Text] [PDF]

				Y. Fukao, Y. Hayashi, S. Mano, M. Hayashi, and M. Nishimura Developmental Analysis of a Putative ATP/ADP Carrier Protein Localized on Glyoxysomal Membranes During the Peroxisome Transition in Pumpkin Cotyledons Plant Cell Physiol., August 1, 2001; 42(8): 835 - 841. [Abstract] [Full Text] [PDF]

				K. Daschner, I. Couee, and S. Binder The Mitochondrial Isovaleryl-Coenzyme A Dehydrogenase of Arabidopsis Oxidizes Intermediates of Leucine and Valine Catabolism Plant Physiology, June 1, 2001; 126(2): 601 - 612. [Abstract] [Full Text] [PDF]

				K. Nito, K. Yamaguchi, M. Kondo, M. Hayashi, and M. Nishimura Pumpkin Peroxisomal Ascorbate Peroxidase is Localized on Peroxisomal Membranes and Unknown Membranous Structures Plant Cell Physiol., January 1, 2001; 42(1): 20 - 27. [Abstract] [Full Text] [PDF]

				B. K. Zolman, A. Yoder, and B. Bartel Genetic Analysis of Indole-3-butyric Acid Responses in Arabidopsis thaliana Reveals Four Mutant Classes Genetics, November 1, 2000; 156(3): 1323 - 1337. [Abstract] [Full Text]

				B. E. Froman, P. C. Edwards, A. G. Bursch, and K. Dehesh ACX3, a Novel Medium-Chain Acyl-Coenzyme A Oxidase from Arabidopsis Plant Physiology, June 1, 2000; 123(2): 733 - 742. [Abstract] [Full Text]

				L. De Bellis, S. Gonzali, A. Alpi, H. Hayashi, M. Hayashi, and M. Nishimura Purification and Characterization of a Novel Pumpkin Short-Chain Acyl-Coenzyme A Oxidase with Structural Similarity to Acyl-Coenzyme A Dehydrogenases Plant Physiology, May 1, 2000; 123(1): 327 - 334. [Abstract] [Full Text]

				P. J. Eastmond, M. A. Hooks, D. Williams, P. Lange, N. Bechtold, C. Sarrobert, L. Nussaume, and I. A. Graham Promoter Trapping of a Novel Medium-chain Acyl-CoA Oxidase, Which Is Induced Transcriptionally during Arabidopsis Seed Germination J. Biol. Chem., October 27, 2000; 275(44): 34375 - 34381. [Abstract] [Full Text] [PDF]