HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Blumenthal, S. G. Articles by Dittmer, J. Search for Related Content PubMed PubMed Citation Articles by Blumenthal, S. G. Articles by Dittmer, J. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 18, 12910-12916, April 30, 1999

Regulation of the Human Interleukin-5 Promoter by Ets Transcription Factors
ETS1 AND ETS2, BUT NOT ELF-1, COOPERATE WITH GATA3 AND HTLV-I TAX₁

Sibylle G. Blumenthal, Gabriele Aichele, Thomas Wirth^§, Armin P. Czernilofsky^¶, Alfred Nordheim, and Jürgen Dittmer

From the  Institut für Zellbiologie, Abteilung Molekularbiologie, Universität Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany, the ^§ Institut für Medizinische Strahlenkunde und Zellforschung (MSZ), Versbacher Strasse 5, 97078 Würzburg, Germany, and the ^¶ Bender + Co Gesellschaft mbH, Dr. Boehringer-Gasse 5-11, 1121 Wien, Austria

Interleukin-5 (IL-5), expressed primarily by type-2 T helper (Th2) cells, plays an important role in the development of allergic diseases, such as allergic asthma. Studying the regulation of IL-5 gene expression by Ets transcription factors, we found that Ets1 and Ets2, but not Elf-1, were able to activate the human IL-5 promoter in Jurkat T-cells. This required the presence of either phorbol 12-myristate acetate (PMA) plus ionomycin or PMA plus the viral protein HTLV-I Tax₁. By mutation studies, it could be shown that Ets1 and Ets2 exerted their effects on the IL-5 promoter through a GGAA motif within the Cle0 element. In myeloid Kasumi cells, Ets1 and Ets2 failed to stimulate IL-5 promoter activity, unless the T-cell specific transcription factor GATA3 was added. These results show, for the first time, that Ets1 and Ets2 are able to cooperate with GATA3. Both ionomycin and Tax₁ increased the combined effect of GATA3 with Ets1 and Ets2 in the presence of PMA. The data further demonstrate that, in addition to Ets1, Ets2 is also able to functionally cooperate with Tax₁. The synergism of GATA3 with either Ets1 or Ets2 may play an important role in calcium- or Tax₁-dependent regulation of IL-5 expression in Th2 cells or in HTLV-I transformed adult T-cell leukemia cells, respectively.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				D. Gaucher, R. Therrien, N. Kettaf, B. R. Angermann, G. Boucher, A. Filali-Mouhim, J. M. Moser, R. S. Mehta, D. R. Drake III, E. Castro, et al. Yellow fever vaccine induces integrated multilineage and polyfunctional immune responses J. Exp. Med., December 22, 2008; 205(13): 3119 - 3131. [Abstract] [Full Text] [PDF]

				V. K. Nagaleekar, S. A. Diehl, I. Juncadella, C. Charland, N. Muthusamy, S. Eaton, L. Haynes, L. A. Garrett-Sinha, J. Anguita, and M. Rincon IP3 Receptor-Mediated Ca2+ Release in Naive CD4 T Cells Dictates Their Cytokine Program J. Immunol., December 15, 2008; 181(12): 8315 - 8322. [Abstract] [Full Text] [PDF]

				S. Klein-Hessling, T. Bopp, M. K. Jha, A. Schmidt, S. Miyatake, E. Schmitt, and E. Serfling Cyclic AMP-induced Chromatin Changes Support the NFATc-mediated Recruitment of GATA-3 to the Interleukin 5 Promoter J. Biol. Chem., November 7, 2008; 283(45): 31030 - 31037. [Abstract] [Full Text] [PDF]

				J. Wang, M. F. Shannon, and I. G. Young A role for Ets1, synergizing with AP-1 and GATA-3 in the regulation of IL-5 transcription in mouse Th2 lymphocytes Int. Immunol., February 1, 2006; 18(2): 313 - 323. [Abstract] [Full Text] [PDF]

				R. Grenningloh, B. Y. Kang, and I-C. Ho Ets-1, a functional cofactor of T-bet, is essential for Th1 inflammatory responses J. Exp. Med., February 22, 2005; 201(4): 615 - 626. [Abstract] [Full Text] [PDF]

				M. D. Dodon, Z. Li, S. Hamaia, and L. Gazzolo Tax protein of human T-cell leukaemia virus type 1 induces interleukin 17 gene expression in T cells J. Gen. Virol., July 1, 2004; 85(7): 1921 - 1932. [Abstract] [Full Text] [PDF]

				M. Arman, J. Calvo, M. E. Trojanowska, P. N. Cockerill, M. Santana, M. Lopez-Cabrera, J. Vives, and F. Lozano Transcriptional Regulation of Human CD5: Important Role of Ets Transcription Factors in CD5 Expression in T Cells J. Immunol., June 15, 2004; 172(12): 7519 - 7529. [Abstract] [Full Text] [PDF]

				Y. Nakamura, S. Esnault, T. Maeda, E. A. B. Kelly, J. S. Malter, and N. N. Jarjour Ets-1 Regulates TNF-{alpha}-Induced Matrix Metalloproteinase-9 and Tenascin Expression in Primary Bronchial Fibroblasts J. Immunol., February 1, 2004; 172(3): 1945 - 1952. [Abstract] [Full Text] [PDF]

				H. Lie-Venema, A. C. Gittenberger-de Groot, L. J.P. van Empel, M. J. Boot, H. Kerkdijk, E. de Kant, and M. C. DeRuiter Ets-1 and Ets-2 Transcription Factors Are Essential for Normal Coronary and Myocardial Development in Chicken Embryos Circ. Res., April 18, 2003; 92(7): 749 - 756. [Abstract] [Full Text] [PDF]

				S. Klein-Hessling, M. K. Jha, B. Santner-Nanan, F. Berberich-Siebelt, T. Baumruker, A. Schimpl, and E. Serfling Protein Kinase A Regulates GATA-3-Dependent Activation of IL-5 Gene Expression in Th2 Cells J. Immunol., March 15, 2003; 170(6): 2956 - 2961. [Abstract] [Full Text] [PDF]

				J. P. Justice, M. T. Borchers, J. J. Lee, W. H. Rowan, Y. Shibata, and M. R. Van Scott Ragweed-induced expression of GATA-3, IL-4, and IL-5 by eosinophils in the lungs of allergic C57BL/6J mice Am J Physiol Lung Cell Mol Physiol, February 1, 2002; 282(2): L302 - L309. [Abstract] [Full Text] [PDF]

				G. T. F. Schwenger, R. Fournier, C. C. Kok, V. A. Mordvinov, D. Yeoman, and C. J. Sanderson GATA-3 Has Dual Regulatory Functions in Human Interleukin-5 Transcription J. Biol. Chem., December 14, 2001; 276(51): 48502 - 48509. [Abstract] [Full Text] [PDF]

				R. K. Lindemann, P. Ballschmieter, A. Nordheim, and J. Dittmer Transforming Growth Factor beta Regulates Parathyroid Hormone-related Protein Expression in MDA-MB-231 Breast Cancer Cells through a Novel Smad/Ets Synergism J. Biol. Chem., November 30, 2001; 276(49): 46661 - 46670. [Abstract] [Full Text] [PDF]

				S. Finotto, G. T. De Sanctis, H. A. Lehr, U. Herz, M. Buerke, M. Schipp, B. Bartsch, R. Atreya, E. Schmitt, P. R. Galle, et al. Treatment of Allergic Airway Inflammation and Hyperresponsiveness by Antisense-induced Local Blockade of GATA-3 Expression J. Exp. Med., May 29, 2001; 193(11): 1247 - 1260. [Abstract] [Full Text] [PDF]

				T. H. Mogensen and S. R. Paludan Molecular Pathways in Virus-Induced Cytokine Production Microbiol. Mol. Biol. Rev., March 1, 2001; 65(1): 131 - 150. [Abstract] [Full Text] [PDF]

				C. G. Garlisi, A. S. Uss, H. Xiao, F. Tian, K. E. Sheridan, L. Wang, M. Motasim Billah, R. W. Egan, K. S. Stranick, and S. P. Umland A Unique mRNA Initiated within a Middle Intron of WHSC1/MMSET Encodes a DNA Binding Protein That Suppresses Human IL-5 Transcription Am. J. Respir. Cell Mol. Biol., January 1, 2001; 24(1): 90 - 98. [Abstract] [Full Text]

				M. S. Salerno, V. A. Mordvinov, and C. J. Sanderson Binding of Octamer Factors to a Novel 3'-Positive Regulatory Element in the Mouse Interleukin-5 Gene J. Biol. Chem., February 11, 2000; 275(6): 4525 - 4531. [Abstract] [Full Text] [PDF]

				D. O. Cowley and B. J. Graves Phosphorylation represses Ets-1 DNA binding by reinforcing autoinhibition Genes & Dev., February 1, 2000; 14(3): 366 - 376. [Abstract] [Full Text]