HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hajduch, E. Articles by Hundal, H. S. Search for Related Content PubMed PubMed Citation Articles by Hajduch, E. Articles by Hundal, H. S. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 19, 13563-13568, May 7, 1999

Serotonin (5-Hydroxytryptamine), a Novel Regulator of Glucose Transport in Rat Skeletal Muscle

Eric Hajduch, Franck Rencurel, Anudharan Balendran, Ian H. Batty^§, C. Peter Downes^§, and Harinder S. Hundal

From the Departments of Anatomy and Physiology and ^§ Biochemistry and the  Medical Research Council Protein Phosphorylation Unit, The University of Dundee, Dundee DD1 4HN, Scotland

In this study we show that serotonin (5-hydroxytryptamine (5-HT)) causes a rapid stimulation in glucose uptake by ~50% in both L6 myotubes and isolated rat skeletal muscle. This activation is mediated via the 5-HT_2A receptor, which is expressed in L6, rat, and human skeletal muscle. In L6 cells, expression of the 5-HT_2A receptor is developmentally regulated based on the finding that receptor abundance increases by over 3-fold during differentiation from myoblasts to myotubes. Stimulation of the 5-HT_2A receptor using methylserotonin (m-HT), a selective 5-HT_2A agonist, increased muscle glucose uptake in a manner similar to that seen in response to 5-HT. The agonist-mediated stimulation in glucose uptake was attributable to an increase in the plasma membrane content of GLUT1, GLUT3, and GLUT4. The stimulatory effects of 5-HT and m-HT were suppressed in the presence of submicromolar concentrations of ketanserin (a selective 5-HT_2A antagonist) providing further evidence that the increase in glucose uptake was specifically mediated via the 5-HT_2A receptor. Treatment of L6 cells with insulin resulted in tyrosine phosphorylation of IRS1, increased cellular production of phosphatidylinositol 3,4,5-phosphate and a 41-fold activation in protein kinase B (PKB/Akt) activity. In contrast, m-HT did not modulate IRS1, phosphoinositide 3-kinase, or PKB activity. The present results indicate that rat and human skeletal muscle both express the 5-HT_2A receptor and that 5-HT and specific 5-HT_2A agonists can rapidly stimulate glucose uptake in skeletal muscle by a mechanism which does not depend upon components that participate in the insulin signaling pathway.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				W S Waring, J Wrate, and D N Bateman Olanzapine overdose is associated with acute muscle toxicity Human and Experimental Toxicology, December 1, 2006; 25(12): 735 - 740. [Abstract] [PDF]

				M. Gilles, A. Wilke, D. Kopf, A. Nonell, H. Lehnert, and M. Deuschle Antagonism of the Serotonin (5-HT)-2 Receptor and Insulin Sensitivity: Implications for Atypical Antipsychotics Psychosom Med, September 1, 2005; 67(5): 748 - 751. [Abstract] [Full Text] [PDF]

				M. C. Moore, K. Kimura, H. Shibata, T. Honjoh, M. Saito, C. A. Everett, M. S. Smith, and A. D. Cherrington Portal 5-hydroxytryptophan infusion enhances glucose disposal in conscious dogs Am J Physiol Endocrinol Metab, August 1, 2005; 289(2): E225 - E231. [Abstract] [Full Text] [PDF]

				D. E. Johnson, H. Yamazaki, K. M. Ward, A. W. Schmidt, W. S. Lebel, J. L. Treadway, E. M. Gibbs, W. S. Zawalich, and H. Rollema Inhibitory Effects of Antipsychotics on Carbachol-Enhanced Insulin Secretion From Perifused Rat Islets: Role of Muscarinic Antagonism in Antipsychotic-Induced Diabetes and Hyperglycemia Diabetes, May 1, 2005; 54(5): 1552 - 1558. [Abstract] [Full Text] [PDF]

				M. C. Moore, C. A. DiCostanzo, D. Dardevet, M. Lautz, B. Farmer, D. W. Neal, and A. D. Cherrington Portal infusion of a selective serotonin reuptake inhibitor enhances hepatic glucose disposal in conscious dogs Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1057 - E1063. [Abstract] [Full Text] [PDF]

				M. C. Moore, W. B. Geho, M. Lautz, B. Farmer, D. W. Neal, and A. D. Cherrington Portal Serotonin Infusion and Glucose Disposal in Conscious Dogs Diabetes, January 1, 2004; 53(1): 14 - 20. [Abstract] [Full Text] [PDF]

				L. M. Ballou, H.-Y. Lin, G. Fan, Y.-P. Jiang, and R. Z. Lin Activated G{alpha}q Inhibits p110{alpha} Phosphatidylinositol 3-Kinase and Akt J. Biol. Chem., June 20, 2003; 278(26): 23472 - 23479. [Abstract] [Full Text] [PDF]

				F. Wappler, M. Fiege, and J. Schulte am Esch Pathophysiological role of the serotonin system in malignant hyperthermia Br. J. Anaesth., November 1, 2001; 87(5): 794 - 798. [Abstract] [Full Text] [PDF]