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J Biol Chem, Vol. 274, Issue 2, 1017-1025, January 8, 1999
,**
From the Infectious Neisseria gonorrhoeae make
relatively large lipooligosaccharides (LOS) that structurally resemble
human glycosphingolipids. MS11mkC is an LOS variant of N. gonorrhoeae strain MS11 which was isolated from men at the onset
of dysuria (Schneider, H., Griffiss, J. M., Boslego, J. W.,
Hitchcock, P. J., Zahos, K. M., and Apicella, M. A. (1991) J. Exp. Med. 174, 1601-1605). Delayed extraction
matrix-assisted laser desorption and ionization and electrospray
ionization mass spectrometry of O-deacylated MS11mkC LOS
produced ions consistent with known LOS which have
lacto-N-neotetraose (Gal Centre for Immunochemistry and the
** Department of Laboratory Medicine, University of
California, San Francisco, California 94121 and the ¶ Department
of Bacterial Diseases, Walter Reed Army Institute of Research,
Washington, D. C. 20307
1
4GlcNAc13Gal14Glc; paraglobosyl; monoclonal
antibodies (mAbs) 1B2+ and 06B4+) and
GalNAclacto-N-neotetraose (gangliosyl; mAb
1-1-M+) oligosaccharides. Ion peaks for a larger LOS which
also bound mAb 1B2 indicated the addition of a hexose (+162 Da) to
gangliosyl LOS or the addition of a hexose and a
N-acetylhexosamine (+365 Da) to paraglobosyl LOS. Analysis
of HF-treated and O-deacylated LOS revealed three major
components present in a phosphoethanolamine (PEA)0 and a
PEA1 series. Digestion of MS11mkC LOS by
-N-acetylhexosaminidase and -galactosidase, alone and
sequentially, combined with mAb binding patterns, confirmed the
presence of a nonreducing terminal repeating LacNAc
((Gal14GlcNAc)2) on the largest LOS, rather than a parallel oligosaccharide structure.
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