HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Niewmierzycka, A. Articles by Clarke, S. Search for Related Content PubMed PubMed Citation Articles by Niewmierzycka, A. Articles by Clarke, S. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 2, 814-824, January 8, 1999

S-Adenosylmethionine-dependent Methylation in Saccharomyces cerevisiae
IDENTIFICATION OF A NOVEL PROTEIN ARGININE METHYLTRANSFERASE

From the Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California 90095-1569

We used sequence motifs conserved in S-adenosylmethionine-dependent methyltransferases to identify 26 putative methyltransferases from the complete genome of the yeast Saccharomyces cerevisiae. Seven sequences with the best matches to the methyltransferase consensus motifs were selected for further study. We prepared yeast disruption mutants of each of the genes encoding these sequences, and we found that disruption of the YJL125c gene is lethal, whereas disruptions of YCR047c and YDR140w lead to slow growth phenotypes. Normal growth was observed when the YDL201w, YDR465c, YHR209w, and YOR240w genes were disrupted. Initial analysis of protein methylation patterns of all mutants by amino acid analysis revealed that the YDR465c mutant has a defect in the methylation of the -nitrogen atom of arginine residues. We propose that YDR465c codes for the methyltransferase responsible for this recently characterized type of protein methylation, and we designate the enzyme as Rmt2 (protein arginine methyltransferase). In addition, we show that the methylation of susceptible residues in Rmt2 substrates is likely to take place on nascent polypeptide chains and that these substrates exist in the cell as fully methylated species. Interestingly, Rmt2 has 27% sequence identity over 138 amino acids to the mammalian guanidinoacetate N-methyltransferase, an enzyme responsible for methylating the -nitrogen of the small molecule guanidinoacetate.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				T. C. Petrossian and S. G. Clarke Multiple Motif Scanning to Identify Methyltransferases from the Yeast Proteome Mol. Cell. Proteomics, July 1, 2009; 8(7): 1516 - 1526. [Abstract] [Full Text] [PDF]

				J. C. Fisk, J. Sayegh, C. Zurita-Lopez, S. Menon, V. Presnyak, S. G. Clarke, and L. K. Read A Type III Protein Arginine Methyltransferase from the Protozoan Parasite Trypanosoma brucei J. Biol. Chem., April 24, 2009; 284(17): 11590 - 11600. [Abstract] [Full Text] [PDF]

				J. White, Z. Li, R. Sardana, J. M. Bujnicki, E. M. Marcotte, and A. W. Johnson Bud23 Methylates G1575 of 18S rRNA and Is Required for Efficient Nuclear Export of Pre-40S Subunits Mol. Cell. Biol., May 15, 2008; 28(10): 3151 - 3161. [Abstract] [Full Text] [PDF]

				J. Sayegh, K. Webb, D. Cheng, M. T. Bedford, and S. G. Clarke Regulation of Protein Arginine Methyltransferase 8 (PRMT8) Activity by Its N-terminal Domain J. Biol. Chem., December 14, 2007; 282(50): 36444 - 36453. [Abstract] [Full Text] [PDF]

				D. A. Pasternack, J. Sayegh, S. Clarke, and L. K. Read Evolutionarily Divergent Type II Protein Arginine Methyltransferase in Trypanosoma brucei Eukaryot. Cell, September 1, 2007; 6(9): 1665 - 1681. [Abstract] [Full Text] [PDF]

				A. E. McBride, C. Zurita-Lopez, A. Regis, E. Blum, A. Conboy, S. Elf, and S. Clarke Protein Arginine Methylation in Candida albicans: Role in Nuclear Transport Eukaryot. Cell, July 1, 2007; 6(7): 1119 - 1129. [Abstract] [Full Text] [PDF]

				F. Bachand Protein Arginine Methyltransferases: from Unicellular Eukaryotes to Humans Eukaryot. Cell, June 1, 2007; 6(6): 889 - 898. [Full Text] [PDF]

				A. Stetler, C. Winograd, J. Sayegh, A. Cheever, E. Patton, X. Zhang, S. Clarke, and S. Ceman Identification and characterization of the methyl arginines in the fragile X mental retardation protein Fmrp Hum. Mol. Genet., January 1, 2006; 15(1): 87 - 96. [Abstract] [Full Text] [PDF]

				J. Lee, J. Sayegh, J. Daniel, S. Clarke, and M. T. Bedford PRMT8, a New Membrane-bound Tissue-specific Member of the Protein Arginine Methyltransferase Family J. Biol. Chem., September 23, 2005; 280(38): 32890 - 32896. [Abstract] [Full Text] [PDF]

				A. E. McBride, J. T. Cook, E. A. Stemmler, K. L. Rutledge, K. A. McGrath, and J. A. Rubens Arginine Methylation of Yeast mRNA-binding Protein Npl3 Directly Affects Its Function, Nuclear Export, and Intranuclear Protein Interactions J. Biol. Chem., September 2, 2005; 280(35): 30888 - 30898. [Abstract] [Full Text] [PDF]

				J. Eichler and M. W. W. Adams Posttranslational Protein Modification in Archaea Microbiol. Mol. Biol. Rev., September 1, 2005; 69(3): 393 - 425. [Abstract] [Full Text] [PDF]

				L. B. W. SZEWCZAK, J. S. GABRIELSEN, S. J. DEGREGORIO, S. A. STROBEL, and J. A. STEITZ Molecular basis for RNA kink-turn recognition by the h15.5K small RNP protein RNA, September 1, 2005; 11(9): 1407 - 1419. [Abstract] [Full Text] [PDF]

				A. ALEXANDROV, E. J. GRAYHACK, and E. M. PHIZICKY tRNA m7G methyltransferase Trm8p/Trm82p: Evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p RNA, May 1, 2005; 11(5): 821 - 830. [Abstract] [Full Text] [PDF]

				A. Zakrzewska, A. Boorsma, S. Brul, K. J. Hellingwerf, and F. M. Klis Transcriptional Response of Saccharomyces cerevisiae to the Plasma Membrane-Perturbing Compound Chitosan Eukaryot. Cell, April 1, 2005; 4(4): 703 - 715. [Abstract] [Full Text] [PDF]

				V. Heurgue-Hamard, S. Champ, L. Mora, T. Merkoulova-Rainon, L. L. Kisselev, and R. H. Buckingham The Glutamine Residue of the Conserved GGQ Motif in Saccharomyces cerevisiae Release Factor eRF1 Is Methylated by the Product of the YDR140w Gene J. Biol. Chem., January 28, 2005; 280(4): 2439 - 2445. [Abstract] [Full Text] [PDF]

				A. K. HENRAS, R. CAPEYROU, Y. HENRY, and M. CAIZERGUES-FERRER Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs RNA, November 18, 2004; 10(11): 1704 - 1712. [Abstract] [Full Text] [PDF]

				C. Lemaire, F. Guibet-Grandmougin, D. Angles, G. Dujardin, and N. Bonnefoy A Yeast Mitochondrial Membrane Methyltransferase-like Protein Can Compensate for oxa1 Mutations J. Biol. Chem., November 12, 2004; 279(46): 47464 - 47472. [Abstract] [Full Text] [PDF]

				G. K. Arhin, S. Shen, H. Irmer, E. Ullu, and C. Tschudi Role of a 300-Kilodalton Nuclear Complex in the Maturation of Trypanosoma brucei Initiator Methionyl-tRNA Eukaryot. Cell, August 1, 2004; 3(4): 893 - 899. [Abstract] [Full Text] [PDF]

				T. B. Miranda, M. Miranda, A. Frankel, and S. Clarke PRMT7 Is a Member of the Protein Arginine Methyltransferase Family with a Distinct Substrate Specificity J. Biol. Chem., May 28, 2004; 279(22): 22902 - 22907. [Abstract] [Full Text] [PDF]

				Q. Fang, J. Peng, and T. Dierks Post-translational Formylglycine Modification of Bacterial Sulfatases by the Radical S-Adenosylmethionine Protein AtsB J. Biol. Chem., April 9, 2004; 279(15): 14570 - 14578. [Abstract] [Full Text] [PDF]

				M. Yanagida, T. Hayano, Y. Yamauchi, T. Shinkawa, T. Natsume, T. Isobe, and N. Takahashi Human Fibrillarin Forms a Sub-complex with Splicing Factor 2-associated p32, Protein Arginine Methyltransferases, and Tubulins {alpha}3 and {beta}1 That Is Independent of Its Association with Preribosomal Ribonucleoprotein Complexes J. Biol. Chem., January 16, 2004; 279(3): 1607 - 1614. [Abstract] [Full Text] [PDF]

				H. R. Kalhor and S. Clarke Novel Methyltransferase for Modified Uridine Residues at the Wobble Position of tRNA Mol. Cell. Biol., December 15, 2003; 23(24): 9283 - 9292. [Abstract] [Full Text] [PDF]

				J. E. Katz, M. Dlakic, and S. Clarke Automated Identification of Putative Methyltransferases from Genomic Open Reading Frames Mol. Cell. Proteomics, August 1, 2003; 2(8): 525 - 540. [Abstract] [Full Text] [PDF]

				J. E. JACKMAN, R. K. MONTANGE, H. S. MALIK, and E. M. PHIZICKY Identification of the yeast gene encoding the tRNA m1G methyltransferase responsible for modification at position 9 RNA, May 1, 2003; 9(5): 574 - 585. [Abstract] [Full Text] [PDF]

				T. S. Rozhdestvensky, T. H. Tang, I. V. Tchirkova, J. Brosius, J.-P. Bachellerie, and A. Huttenhofer Binding of L7Ae protein to the K-turn of archaeal snoRNAs: a shared RNA binding motif for C/D and H/ACA box snoRNAs in Archaea Nucleic Acids Res., February 1, 2003; 31(3): 869 - 877. [Abstract] [Full Text] [PDF]

				L. Gros, C. Delaporte, S. Frey, J. Decesse, B. R. de Saint-Vincent, L. Cavarec, A. Dubart, A. V. Gudkov, and A. Jacquemin-Sablon Identification of New Drug Sensitivity Genes Using Genetic Suppressor Elements: Protein Arginine N-Methyltransferase Mediates Cell Sensitivity to DNA-damaging Agents Cancer Res., January 1, 2003; 63(1): 164 - 171. [Abstract] [Full Text] [PDF]

				S. Galardi, A. Fatica, A. Bachi, A. Scaloni, C. Presutti, and I. Bozzoni Purified Box C/D snoRNPs Are Able To Reproduce Site-Specific 2'-O-Methylation of Target RNA In Vitro Mol. Cell. Biol., October 1, 2002; 22(19): 6663 - 6668. [Abstract] [Full Text] [PDF]

				S. Fujiyama, M. Yanagida, T. Hayano, Y. Miura, T. Isobe, and N. Takahashi Isolation and Proteomic Characterization of Human Parvulin-associating Preribosomal Ribonucleoprotein Complexes J. Biol. Chem., June 21, 2002; 277(26): 23773 - 23780. [Abstract] [Full Text] [PDF]

				N. Tomasevic and B. A. Peculis Xenopus LSm Proteins Bind U8 snoRNA via an Internal Evolutionarily Conserved Octamer Sequence Mol. Cell. Biol., June 15, 2002; 22(12): 4101 - 4112. [Abstract] [Full Text] [PDF]

				H. H. Ng, Q. Feng, H. Wang, H. Erdjument-Bromage, P. Tempst, Y. Zhang, and K. Struhl Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association Genes & Dev., June 15, 2002; 16(12): 1518 - 1527. [Abstract] [Full Text] [PDF]

				M.-K. Chern, K.-N. Chang, L.-F. Liu, T.-C. S. Tam, Y.-C. Liu, Y.-L. Liang, and M. F. Tam Yeast Ribosomal Protein L12 Is a Substrate of Protein-arginine Methyltransferase 2 J. Biol. Chem., May 3, 2002; 277(18): 15345 - 15353. [Abstract] [Full Text] [PDF]

				S. Clarke The methylator meets the terminator PNAS, February 5, 2002; 99(3): 1104 - 1106. [Full Text] [PDF]

				A. Frankel, N. Yadav, J. Lee, T. L. Branscombe, S. Clarke, and M. T. Bedford The Novel Human Protein Arginine N-Methyltransferase PRMT6 Is a Nuclear Enzyme Displaying Unique Substrate Specificity J. Biol. Chem., January 25, 2002; 277(5): 3537 - 3543. [Abstract] [Full Text] [PDF]

				K. W. Jones, K. Gorzynski, C. M. Hales, U. Fischer, F. Badbanchi, R. M. Terns, and M. P. Terns Direct Interaction of the Spinal Muscular Atrophy Disease Protein SMN with the Small Nucleolar RNA-associated Protein Fibrillarin J. Biol. Chem., October 12, 2001; 276(42): 38645 - 38651. [Abstract] [Full Text] [PDF]

				X. Cheng and R. J. Roberts AdoMet-dependent methylation, DNA methyltransferases and base flipping Nucleic Acids Res., September 15, 2001; 29(18): 3784 - 3795. [Abstract] [Full Text] [PDF]

				B. Hong, K. Wu, J. S. Brockenbrough, P. Wu, and J. P. Aris Temperature sensitive nop2 alleles defective in synthesis of 25S rRNA and large ribosomal subunits in Saccharomyces cerevisiae Nucleic Acids Res., July 15, 2001; 29(14): 2927 - 2937. [Abstract] [Full Text] [PDF]

				H. J. Sofia, G. Chen, B. G. Hetzler, J. F. Reyes-Spindola, and N. E. Miller Radical SAM, a novel protein superfamily linking unresolved steps in familiar biosynthetic pathways with radical mechanisms: functional characterization using new analysis and information visualization methods Nucleic Acids Res., March 1, 2001; 29(5): 1097 - 1106. [Abstract] [Full Text] [PDF]

				S.A. GERBI, A.V. BOROVJAGIN, M. EZROKHI, and T.S. LANGE Ribosome Biogenesis: Role of Small Nucleolar RNA in Maturation of Eukaryotic rRNA Cold Spring Harb Symp Quant Biol, January 1, 2001; 66(0): 575 - 590. [Abstract] [PDF]

				W. A. Speckmann, R. M. Terns, and M. P. Terns The Box C/D motif directs snoRNA 5'-cap hypermethylation Nucleic Acids Res., November 15, 2000; 28(22): 4467 - 4473. [Abstract] [Full Text] [PDF]

				Snaar, Wiesmeijer, A. Jochemsen, H. Tanke, and R. Dirks Mutational Analysis of Fibrillarin and Its Mobility in Living Human Cells J. Cell Biol., October 30, 2000; 151(3): 653 - 662. [Abstract] [Full Text] [PDF]

				A. Colley, J. D. Beggs, D. Tollervey, and D. L. J. Lafontaine Dhr1p, a Putative DEAH-Box RNA Helicase, Is Associated with the Box C+D snoRNP U3 Mol. Cell. Biol., October 1, 2000; 20(19): 7238 - 7246. [Abstract] [Full Text]

				B. Schwer, N. Saha, X. Mao, H.-W. Chen, and S. Shuman Structure-Function Analysis of Yeast mRNA Cap Methyltransferase and High-Copy Suppression of Conditional Mutants by AdoMet Synthase and the Ubiquitin Conjugating Enzyme Cdc34p Genetics, August 1, 2000; 155(4): 1561 - 1576. [Abstract] [Full Text]

				D. L. J. Lafontaine and D. Tollervey Synthesis and Assembly of the Box C+D Small Nucleolar RNPs Mol. Cell. Biol., April 15, 2000; 20(8): 2650 - 2659. [Abstract] [Full Text]

				B. E. Jady and T. Kiss Characterisation of the U83 and U84 small nucleolar RNAs: two novel 2'-O-ribose methylation guide RNAs that lack complementarities to ribosomal RNAs Nucleic Acids Res., March 15, 2000; 28(6): 1348 - 1354. [Abstract] [Full Text] [PDF]

				L. Pintard, D. Kressler, and B. Lapeyre Spb1p Is a Yeast Nucleolar Protein Associated with Nop1p and Nop58p That Is Able To Bind S-Adenosyl-L-Methionine In Vitro Mol. Cell. Biol., February 15, 2000; 20(4): 1370 - 1381. [Abstract] [Full Text]

				T. Selmer, J. Kahnt, M. Goubeaud, S. Shima, W. Grabarse, U. Ermler, and R. K. Thauer The Biosynthesis of Methylated Amino Acids in the Active Site Region of Methyl-coenzyme M Reductase J. Biol. Chem., February 11, 2000; 275(6): 3755 - 3760. [Abstract] [Full Text] [PDF]

				A. E. McBride, V. H. Weiss, H. K. Kim, J. M. Hogle, and P. A. Silver Analysis of the Yeast Arginine Methyltransferase Hmt1p/Rmt1p and Its in Vivo Function. COFACTOR BINDING AND SUBSTRATE INTERACTIONS J. Biol. Chem., February 4, 2000; 275(5): 3128 - 3136. [Abstract] [Full Text] [PDF]

				D. Kressler, P. Linder, and J. de la Cruz Protein trans-Acting Factors Involved in Ribosome Biogenesis in Saccharomyces cerevisiae Mol. Cell. Biol., December 1, 1999; 19(12): 7897 - 7912. [Full Text] [PDF]

				P. Ganot, B. E. Jady, M.-L. Bortolin, X. Darzacq, and T. Kiss Nucleolar Factors Direct the 2'-O-Ribose Methylation and Pseudouridylation of U6 Spliceosomal RNA Mol. Cell. Biol., October 1, 1999; 19(10): 6906 - 6917. [Abstract] [Full Text] [PDF]

				D. A. Dunbar, S. Wormsley, T. M. Lowe, and S. J. Baserga Fibrillarin-associated Box C/D Small Nucleolar RNAs in Trypanosoma brucei. SEQUENCE CONSERVATION AND IMPLICATIONS FOR 2'-O-RIBOSE METHYLATION OF rRNA J. Biol. Chem., May 5, 2000; 275(19): 14767 - 14776. [Abstract] [Full Text] [PDF]

				B. Polevoda, M. R. Martzen, B. Das, E. M. Phizicky, and F. Sherman Cytochrome c Methyltransferase, Ctm1p, of Yeast J. Biol. Chem., June 30, 2000; 275(27): 20508 - 20513. [Abstract] [Full Text] [PDF]

				F. Barneche, F. Steinmetz, and M. Echeverria Fibrillarin Genes Encode Both a Conserved Nucleolar Protein and a Novel Small Nucleolar RNA Involved in Ribosomal RNA Methylation in Arabidopsis thaliana J. Biol. Chem., August 25, 2000; 275(35): 27212 - 27220. [Abstract] [Full Text] [PDF]

				T. L. Branscombe, A. Frankel, J.-H. Lee, J. R. Cook, Z.-h. Yang, S. Pestka, and S. Clarke PRMT5 (Janus Kinase-binding Protein 1) Catalyzes the Formation of Symmetric Dimethylarginine Residues in Proteins J. Biol. Chem., August 24, 2001; 276(35): 32971 - 32976. [Abstract] [Full Text] [PDF]

				J. Anderson, L. Phan, and A. G. Hinnebusch The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae PNAS, May 9, 2000; 97(10): 5173 - 5178. [Abstract] [Full Text] [PDF]