|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 20, 14079-14089, May 14, 1999
From the Ruttenberg Cancer Center, Mount Sinai School of Medicine,
New York, New York 10029-6574
To identify mechanisms whereby activating
transcription factor 2 (ATF2) alters the radiation resistance of human
melanoma cells, we examined the possible role of ATF2 in UVC-induced
apoptosis. Forced expression of full-length or truncated (
Down-regulation of Tumor Necrosis Factor
Expression by
Activating Transcription Factor 2 Increases UVC-induced Apoptosis of
Late-stage Melanoma Cells
1-195
amino acids) forms of ATF2 in LU1205, a late-stage human melanoma cell
line, elevated the levels of UVC-induced apoptosis. At the same time, either truncated or full-length forms of ATF2 reduced UVC-induced activation of the tumor necrosis factor-
(TNF) promoter and decreased expression of TNF. Forced expression of c-Jun in
ATF2-expressing melanoma cells restored TNF expression, suggesting
that both forms of ATF2 sequestered transcription factors that
positively regulate TNF expression in response to UV irradiation.
Antagonistic antibodies to Fas, but not to TNFR1, efficiently
suppressed UVC-induced apoptosis, suggesting that the Fas pathway
mediates the primary apoptotic signal in melanoma cells whereas the
TNFR1 pathway elicits a survival signal. Indeed, treatment of melanoma
cells with TNF before UVC irradiation partially suppressed
UVC-induced apoptosis, further supporting the protective role of TNF
in UVC-treated melanoma cells. Taken together, our findings suggest
that ATF2 contributes to UVC-induced apoptosis through transcriptional
silencing of TNF, which balances Fas-mediated cell death in melanoma.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  O Gubbay, M T Rae, A S McNeilly, F X Donadeu, A J Zeleznik, and S G Hillier cAMP response element-binding (CREB) signalling and ovarian surface epithelial cell survival. J. Endocrinol., October 1, 2006; 191(1): 275 - 285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bhoumik, L. Gangi, and Z. Ronai Inhibition of Melanoma Growth and Metastasis by ATF2-Derived Peptides Cancer Res., November 15, 2004; 64(22): 8222 - 8230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Zhu, Y. Zhang, A. M. Bode, and Z. Dong Involvement of ERKs and mitogen- and stress-activated protein kinase in UVC-induced phosphorylation of ATF2 in JB6 cells Carcinogenesis, October 1, 2004; 25(10): 1847 - 1852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sevilla, C. R. Santos, F. M. Vega, and P. A. Lazo Human Vaccinia-related Kinase 1 (VRK1) Activates the ATF2 Transcriptional Activity by Novel Phosphorylation on Thr-73 and Ser-62 and Cooperates with JNK J. Biol. Chem., June 25, 2004; 279(26): 27458 - 27465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hong, H. M. Choi, M. J. Park, Y. H. Kim, Y. H. Choi, H. H. Kim, Y. H. Choi, and J. Cheong Activation and Interaction of ATF2 with the Coactivator ASC-2 Are Responsive for Granulocytic Differentiation by Retinoic Acid J. Biol. Chem., April 23, 2004; 279(17): 16996 - 17003. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bhoumik, N. Jones, and Z. Ronai Transcriptional switch by activating transcription factor 2-derived peptide sensitizes melanoma cells to apoptosis and inhibits their tumorigenicity PNAS, March 23, 2004; 101(12): 4222 - 4227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nakayama, M. Kimura, A. Wada, K. Yahiro, K.-i. Ogushi, T. Niidome, A. Fujikawa, D. Shirasaka, N. Aoyama, H. Kurazono, et al. Helicobacter pylori VacA Activates the p38/Activating Transcription Factor 2-mediated Signal Pathway in AZ-521 Cells J. Biol. Chem., February 20, 2004; 279(8): 7024 - 7028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hayakawa, C. Depatie, M. Ohmichi, and D. Mercola The Activation of c-Jun NH2-terminal Kinase (JNK) by DNA-damaging Agents Serves to Promote Drug Resistance via Activating Transcription Factor 2 (ATF2)-dependent Enhanced DNA Repair J. Biol. Chem., May 30, 2003; 278(23): 20582 - 20592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. N. Ivanov, P. L. Bergami, G. Maulit, T.-A. Sato, D. Sassoon, and Z.'e. Ronai FAP-1 Association with Fas (Apo-1) Inhibits Fas Expression on the Cell Surface Mol. Cell. Biol., May 15, 2003; 23(10): 3623 - 3635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Waetzig and T. Herdegen A Single c-Jun N-terminal Kinase Isoform (JNK3-p54) Is an Effector in Both Neuronal Differentiation and Cell Death J. Biol. Chem., January 3, 2003; 278(1): 567 - 572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. N. Ivanov, M. Krasilnikov, and Z.'e. Ronai Regulation of Fas Expression by STAT3 and c-Jun Is Mediated by Phosphatidylinositol 3-Kinase-AKT Signaling J. Biol. Chem., February 8, 2002; 277(7): 4932 - 4944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-G. Cho, A. Bhoumik, L. Broday, V. Ivanov, B. Rosenstein, and Z.'e. Ronai TIP49b, a Regulator of Activating Transcription Factor 2 Response to Stress and DNA Damage Mol. Cell. Biol., December 15, 2001; 21(24): 8398 - 8413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bhoumik, V. Ivanov, and Z.'e. Ronai Activating Transcription Factor 2-derived Peptides Alter Resistance of Human Tumor Cell Lines to Ultraviolet Irradiation and Chemical Treatment Clin. Cancer Res., February 1, 2001; 7(2): 331 - 342. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Houvras, M. Benezra, H. Zhang, J. J. Manfredi, B. L. Weber, and J. D. Licht BRCA1 Physically and Functionally Interacts with ATF1 J. Biol. Chem., November 10, 2000; 275(46): 36230 - 36237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-F. Shih, Y.-H. Wu, C.-H. Hung, H.-Y. Yang, and J.-Y. Lin Abrin Triggers Cell Death by Inactivating a Thiol-specific Antioxidant Protein J. Biol. Chem., June 8, 2001; 276(24): 21870 - 21877. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |