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J Biol Chem, Vol. 274, Issue 20, 14331-14336, May 14, 1999
Heterodimeric Interactions between Chicken Ovalbumin Upstream
Promoter-Transcription Factor Family Members ARP1 and Ear2
Dorina
Avram ,
Jane E.
Ishmael ,
Daniel J.
Nevrivy §,
Valerie J.
Peterson ,
Suk-Hyung
Lee ,
Paul
Dowell §, and
Mark
Leid §
From the Laboratory of Molecular Pharmacology,
College of Pharmacy and § Program in Molecular and Cellular
Biology, Oregon State University, Corvallis, Oregon 97331-3507
Members of the chicken ovalbumin upstream
promoter-transcription factor (COUP-TF) subfamily of orphan nuclear
receptors, which minimally includes COUP-TFI and ARP1, are highly
expressed in brain and are generally considered to be constitutive
repressors of transcription. We have used a yeast two-hybrid system to
isolate proteins expressed in brain that interact with ARP1. One of the proteins isolated in this screen was Ear2, another orphan receptor that
has been suggested to be a member of the COUP-TF subfamily. Here we
demonstrate that ARP1 and Ear2 form heterodimers in solution and on
directly repeated response elements with high efficiency and a
specificity differing from that of homodimeric complexes composed of
either receptor. ARP1 and Ear2 were observed to interact in mammalian
cells, and the tissue distribution of Ear2 transcripts was found to
overlap precisely with the expression pattern of ARP1 in several mouse
tissues and embryonal carcinoma cell lines. Heterodimeric interactions
between ARP1 and Ear2 may define a distinct pathway of orphan receptor signaling.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

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Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.
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