JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schmidt, M.
Right arrow Articles by Jakobs, K. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schmidt, M.
Right arrow Articles by Jakobs, K. H.

J Biol Chem, Vol. 274, Issue 21, 14648-14654, May 21, 1999

A Role for Rho-kinase in Rho-controlled Phospholipase D Stimulation by the m3 Muscarinic Acetylcholine Receptor

Martina SchmidtDagger , Matthias VoßDagger , Paschal A. Oude WeerninkDagger , Julia WetzelDagger , Mutsuki Amano, Kozo Kaibuchi, and Karl H. JakobsDagger

From the Dagger  Institut für Pharmakologie, Universitätsklinikum Essen, D-45122 Essen, Germany and the  Division of Signal Transduction, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-01, Japan

Stimulation of phospholipase D (PLD) by membrane receptors is now recognized as a major signal transduction pathway involved in diverse cellular functions. Rho proteins control receptor signaling to PLD, and these GTPases have been shown to directly stimulate purified recombinant PLD1 enzymes in vitro. Here we report that stimulation of PLD activity, measured in the presence of phosphatidylinositol 4,5-bisphosphate, by RhoA in membranes of HEK-293 cells expressing the m3 muscarinic acetylcholine receptor (mAChR) is phosphorylation-dependent. Therefore, the possible involvement of the RhoA-stimulated serine/threonine kinase, Rho-kinase, was investigated. Overexpression of Rho-kinase and constitutively active Rho-kinase (Rho-kinase-CAT) but not of kinase-deficient Rho-kinase-CAT markedly increased m3 mAChR-mediated but not protein kinase C-mediated PLD stimulation, similar to overexpression of RhoA. Expression of the Rho-inactivating C3 transferase abrogated the stimulatory effect of wild-type Rho-kinase, but not of Rho-kinase-CAT. Recombinant Rho-kinase-CAT mimicked the phosphorylation-dependent PLD stimulation by RhoA in HEK-293 cell membranes. Finally, the Rho-kinase inhibitor HA-1077 largely inhibited RhoA-induced PLD stimulation in membranes as well as PLD stimulation by the m3 mAChR but not by protein kinase C in intact HEK-293 cells. We conclude that Rho-kinase is involved in Rho-dependent PLD stimulation by the G protein-coupled m3 mAChR in HEK-293 cells. Thus, our findings identify Rho-kinase as a novel player in the receptor-controlled PLD signaling pathway.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
J. Gomez-Cambronero, M. Di Fulvio, and K. Knapek
Understanding phospholipase D (PLD) using leukocytes: PLD involvement in cell adhesion and chemotaxis
J. Leukoc. Biol., August 1, 2007; 82(2): 272 - 281.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. H. Lee, Y. M. Kim, N. W. Kim, J. W. Kim, E. Her, B. K. Kim, J. H. Kim, S. H. Ryu, J. W. Park, D. W. Seo, et al.
Phospholipase D2 acts as an essential adaptor protein in the activation of Syk in antigen-stimulated mast cells
Blood, August 1, 2006; 108(3): 956 - 964.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
W. S. Choi, T. Hiragun, J. H. Lee, Y. M. Kim, H.-P. Kim, A. Chahdi, E. Her, J. W. Han, and M. A. Beaven
Activation of RBL-2H3 Mast Cells Is Dependent on Tyrosine Phosphorylation of Phospholipase D2 by Fyn and Fgr
Mol. Cell. Biol., August 15, 2004; 24(16): 6980 - 6992.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. A. O. Weernink, K. Meletiadis, S. Hommeltenberg, M. Hinz, H. Ishihara, M. Schmidt, and K. H. Jakobs
Activation of Type I Phosphatidylinositol 4-Phosphate 5-Kinase Isoforms by the Rho GTPases, RhoA, Rac1, and Cdc42
J. Biol. Chem., February 27, 2004; 279(9): 7840 - 7849.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
S. Rattan, R. N. Puri, and Y.-P. Fan
Involvement of Rho and Rho-Associated Kinase in Sphincteric Smooth Muscle Contraction by Angiotensin II
Experimental Biology and Medicine, September 1, 2003; 228(8): 972 - 981.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Chahdi, W. S. Choi, Y. M. Kim, and M. A. Beaven
Mastoparan Selectively Activates Phospholipase D2 in Cell Membranes
J. Biol. Chem., March 28, 2003; 278(14): 12039 - 12045.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
J.-H. Parmentier, A. Ahmed, Y. Ruan, G. K. Gandhi, A. E. Saeed, and K. U. Malik
Calcium and Protein Kinase C (PKC)-Related Kinase Mediate alpha 1A-Adrenergic Receptor-Stimulated Activation of Phospholipase D in Rat-1 Cells, Independent of PKC
J. Pharmacol. Exp. Ther., December 1, 2002; 303(3): 1206 - 1215.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
C. Heneweer, L. H. Kruse, F. Kindhauser, M. Schmidt, K. H. Jakobs, H.-W. Denker, and M. Thie
Adhesiveness of human uterine epithelial RL95-2 cells to trophoblast: Rho protein regulation
Mol. Hum. Reprod., November 1, 2002; 8(11): 1014 - 1022.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
W. S. CHOI, A. CHAHDI, Y. M. KIM, P. F. FRAUNDORFER, and M. A. BEAVEN
Regulation of Phospholipase D and Secretion in Mast Cells by Protein Kinase A and Other Protein Kinases
Ann. N.Y. Acad. Sci., June 1, 2002; 968(1): 198 - 212.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
W. S. Choi, Y. M. Kim, C. Combs, M. A. Frohman, and M. A. Beaven
Phospholipases D1 and D2 Regulate Different Phases of Exocytosis in Mast Cells
J. Immunol., June 1, 2002; 168(11): 5682 - 5689.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Wilde, H. Barth, P. Sehr, L. Han, M. Schmidt, I. Just, and K. Aktories
Interaction of the Rho-ADP-ribosylating C3 Exoenzyme with RalA
J. Biol. Chem., April 19, 2002; 277(17): 14771 - 14776.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Skippen, D. H. Jones, C. P. Morgan, M. Li, and S. Cockcroft
Mechanism of ADP Ribosylation Factor-stimulated Phosphatidylinositol 4,5-Bisphosphate Synthesis in HL60 Cells
J. Biol. Chem., February 15, 2002; 277(8): 5823 - 5831.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Yuan, L. W. Slice, and E. Rozengurt
Activation of Protein Kinase D by Signaling through Rho and the alpha Subunit of the Heterotrimeric G Protein G13
J. Biol. Chem., October 12, 2001; 276(42): 38619 - 38627.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. U. Schraufstatter, J. Chung, and M. Burger
IL-8 activates endothelial cell CXCR1 and CXCR2 through Rho and Rac signaling pathways
Am J Physiol Lung Cell Mol Physiol, June 1, 2001; 280(6): L1094 - L1103.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. C. J. Oprins, C. van der Burg, H. P. Meijer, T. Munnik, and J. A. Groot
PLD pathway involved in carbachol-induced Cl{-} secretion: possible role of TNF-{alpha}
Am J Physiol Cell Physiol, April 1, 2001; 280(4): C789 - C795.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
K. S. Murthy, H. Zhou, J. R. Grider, and G. M. Makhlouf
Sequential activation of heterotrimeric and monomeric G proteins mediates PLD activity in smooth muscle
Am J Physiol Gastrointest Liver Physiol, March 1, 2001; 280(3): G381 - G388.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. A. Lunn, H. Wong, E. Rozengurt, and J. H. Walsh
Requirement of cortical actin organization for bombesin, endothelin, and EGF receptor internalization
Am J Physiol Cell Physiol, December 1, 2000; 279(6): C2019 - C2027.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
G. Du, Y. M. Altshuller, Y. Kim, J. M. Han, S. H. Ryu, A. J. Morris, and M. A. Frohman
Dual Requirement for Rho and Protein Kinase C in Direct Activation of Phospholipase D1 Through G Protein-coupled Receptor Signaling
Mol. Biol. Cell, December 1, 2000; 11(12): 4359 - 4368.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
P. A. O. Weernink, P. Schulte, Y. Guo, J. Wetzel, M. Amano, K. Kaibuchi, S. Haverland, M. Vo{beta}, M. Schmidt, G. W. Mayr, et al.
Stimulation of Phosphatidylinositol-4-phosphate 5-Kinase by Rho-Kinase
J. Biol. Chem., March 31, 2000; 275(14): 10168 - 10174.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Vo{beta}, P. A. O. Weernink, S. Haupenthal, U. Moller, R. H. Cool, B. Bauer, J. H. Camonis, K. H. Jakobs, and M. Schmidt
Phospholipase D Stimulation by Receptor Tyrosine Kinases Mediated by Protein Kinase C and a Ras/Ral Signaling Cascade
J. Biol. Chem., December 3, 1999; 274(49): 34691 - 34698.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Schmidt, M. Frings, M.-L. Mono, Y. Guo, P. A. O. Weernink, S. Evellin, L. Han, and K. H. Jakobs
G Protein-coupled Receptor-induced Sensitization of Phospholipase C Stimulation by Receptor Tyrosine Kinases
J. Biol. Chem., October 13, 2000; 275(42): 32603 - 32610.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
U. Rumenapp, M. Asmus, H. Schablowski, M. Woznicki, L. Han, K. H. Jakobs, M. Fahimi-Vahid, C. Michalek, T. Wieland, and M. Schmidt
The M3 Muscarinic Acetylcholine Receptor Expressed in HEK-293 Cells Signals to Phospholipase D via G12 but Not Gq-type G Proteins. REGULATORS OF G PROTEINS AS TOOLS TO DISSECT PERTUSSIS TOXIN-RESISTANT G PROTEINS IN RECEPTOR-EFFECTOR COUPLING
J. Biol. Chem., January 19, 2001; 276(4): 2474 - 2479.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.