| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 21, 14670-14677, May 21, 1999
/HCO3
Exchange in Mouse Submandibular and Pancreatic Ducts
,
From the We have demonstrated previously the regulation of
Cl Department of Pharmacology, Yonsei
University College of Medicine, Seoul 120-752, Korea, and the
¶ Department of Physiology and the Program in Molecular
Biophysics, University of Texas Southwestern Medical Center,
Dallas, Texas 75235
/HCO3 exchange
activity by the cystic fibrosis transmembrane conductance regulator
(CFTR) in model systems of cells stably or transiently transfected with
CFTR (Lee, M. G., Wigley, W. C., Zeng, W., Noel, L. E.,
Marino, C. R., Thomas, P. J., and Muallem, S. (1999)
J. Biol. Chem. 274, 3414-3421). In the present work
we examine the significance of this regulation in cells naturally
expressing CFTR. These include the human colonic T84 cell line and the
mouse submandibular gland and pancreatic ducts, tissues that express high levels of CFTR in the luminal membrane. As in heterologous expression systems, stimulation of T84 cells with forskolin increased the Cl/HCO3 exchange
activity independently of CFTR Cl channel activity.
Freshly isolated submandibular gland ducts from wild type mice showed
variable Cl/HCO3
exchange activity. Measurement of
[Cl]i revealed that this was
largely the result of variable steady-state
[Cl]i. Membrane depolarization
with 5 mM Ba2+ or 100 mM
K+ increased and stabilized
[Cl]i. Under depolarized
conditions wild type and 
F/F mice had comparable basal
Cl/HCO3 exchange
activity. Notably, stimulation with forskolin increased Cl/HCO3 exchange
activity in submandibular gland ducts from wild type but not F/F
mice. Microperfusion of the main pancreatic duct showed
Cl/HCO3 exchange
activity in both the basolateral and luminal membranes. Stimulation of
ducts from wild type animals with forskolin had no effect on
basolateral but markedly stimulated luminal
Cl/HCO3 exchange
activity. By contrast, forskolin had no effect on either basolateral or
luminal Cl/HCO3
exchange activity of ducts from F/F animals. We conclude that CFTR regulates luminal
Cl/HCO3 exchange
activity in CFTR-expressing cells, and we discuss the possible
physiological significance of these findings regarding cystic fibrosis.
This article has been cited by other articles:
|
|
 |
|
  T. Nakamoto, A. Srivastava, V. G. Romanenko, C. E. Ovitt, P. Perez-Cornejo, J. Arreola, T. Begenisich, and J. E. Melvin Functional and molecular characterization of the fluid secretion mechanism in human parotid acinar cells Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2007; 292(6): R2380 - R2390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li, N.-Y. Koo, I.-H. Cho, T.-H. Kwon, S.-Y. Choi, S. J. Lee, S. B. Oh, J.-S. Kim, and K. Park Expression of the Na+-HCO3- cotransporter and its role in pHi regulation in guinea pig salivary glands Am J Physiol Gastrointest Liver Physiol, December 1, 2006; 291(6): G1031 - G1040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Lamprecht and U. Seidler The emerging role of PDZ adapter proteins for regulation of intestinal ion transport Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G766 - G777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. Carlin, R. L. Sedlacek, R. R. Quesnell, F. Pierucci-Alves, D. M. Grieger, and B. D. Schultz PVD9902, a porcine vas deferens epithelial cell line that exhibits neurotransmitter-stimulated anion secretion and expresses numerous HCO3- transporters Am J Physiol Cell Physiol, June 1, 2006; 290(6): C1560 - C1571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Worrell, A. Best, O. R. Crawford, J. Xu, M. Soleimani, and J. B. Matthews Apical ammonium inhibition of cAMP-stimulated secretion in T84 cells is bicarbonate dependent Am J Physiol Gastrointest Liver Physiol, October 1, 2005; 289(4): G768 - G778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Simpson, L. R. Gawenis, N. M. Walker, K. T. Boyle, and L. L. Clarke Chloride conductance of CFTR facilitates basal Cl-/HCO3- exchange in the villous epithelium of intact murine duodenum Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1241 - G1251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. H. Kim, N. Shcheynikov, Y. Wang, and S. Muallem SLC26A7 Is a Cl- Channel Regulated by Intracellular pH J. Biol. Chem., February 25, 2005; 280(8): 6463 - 6470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Pruliere-Escabasse, P. Fanen, A. C. Dazy, E. Lechapt-Zalcman, D. Rideau, A. Edelman, E. Escudier, and A. Coste TGF-{beta}1 downregulates CFTR expression and function in nasal polyps of non-CF patients Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L77 - L83. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Wagner, K. E. Finberg, S. Breton, V. Marshansky, D. Brown, and J. P. Geibel Renal Vacuolar H+-ATPase Physiol Rev, October 1, 2004; 84(4): 1263 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Fernandez-Salazar, P. Pascua, J. J. Calvo, M. A. Lopez, R. M. Case, M. C. Steward, and J. I. San Roman Basolateral anion transport mechanisms underlying fluid secretion by mouse, rat and guinea-pig pancreatic ducts J. Physiol., April 15, 2004; 556(2): 415 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. Kim, W. Han, W. Namkung, J. H. Lee, K. H. Kim, H. Shin, E. Kim, and M. G. Lee Inhibitory Regulation of Cystic Fibrosis Transmembrane Conductance Regulator Anion-transporting Activities by Shank2 J. Biol. Chem., March 12, 2004; 279(11): 10389 - 10396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Bucheimer and J. Linden Purinergic regulation of epithelial transport J. Physiol., March 1, 2004; 555(2): 311 - 321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-V. Nguyen, A. Stuart-Tilley, S. L. Alper, and J. E. Melvin Cl-/HCO3- exchange is acetazolamide sensitive and activated by a muscarinic receptor-induced [Ca2+]i increase in salivary acinar cells Am J Physiol Gastrointest Liver Physiol, February 1, 2004; 286(2): G312 - G320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Lee, J. H. Choi, W. Namkung, J. W. Hanrahan, J. Chang, S. Y. Song, S. W. Park, D. S. Kim, J.-H. Yoon, Y. Suh, et al. A haplotype-based molecular analysis of CFTR mutations associated with respiratory and pancreatic diseases Hum. Mol. Genet., September 15, 2003; 12(18): 2321 - 2332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hegyi, M. A. Gray, and B. E. Argent Substance P inhibits bicarbonate secretion from guinea pig pancreatic ducts by modulating an anion exchanger Am J Physiol Cell Physiol, August 1, 2003; 285(2): C268 - C276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lohi, G. Lamprecht, D. Markovich, A. Heil, M. Kujala, U. Seidler, and J. Kere Isoforms of SLC26A6 mediate anion transport and have functional PDZ interaction domains Am J Physiol Cell Physiol, March 1, 2003; 284(3): C769 - C779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Namkung, J. A. Lee, W. Ahn, W. Han, S. W. Kwon, D. S. Ahn, K. H. Kim, and M. G. Lee Ca2+ Activates Cystic Fibrosis Transmembrane Conductance Regulator- and Cl--dependent HCO3 Transport in Pancreatic Duct Cells J. Biol. Chem., January 3, 2003; 278(1): 200 - 207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. McShane, J.C. Davies, M.G. Davies, A. Bush, D.M. Geddes, and E.W.F.W. Alton Airway surface pH in subjects with cystic fibrosis Eur. Respir. J., January 1, 2003; 21(1): 37 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Verkman, Y. Song, and J. R. Thiagarajah Role of airway surface liquid and submucosal glands in cystic fibrosis lung disease Am J Physiol Cell Physiol, January 1, 2003; 284(1): C2 - C15. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Park, S. B. H. Ko, J. Y. Choi, G. Muallem, P. J. Thomas, A. Pushkin, M.-S. Lee, J. Y. Kim, M. G. Lee, S. Muallem, et al. The Cystic Fibrosis Transmembrane Conductance Regulator Interacts with and Regulates the Activity of the HCO3- Salvage Transporter Human Na+-HCO3- Cotransport Isoform 3 J. Biol. Chem., December 20, 2002; 277(52): 50503 - 50509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Devuyst and W. B. Guggino Chloride channels in the kidney: lessons learned from knockout animals Am J Physiol Renal Physiol, December 1, 2002; 283(6): F1176 - F1191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Tate, G MacGregor, M Davis, J A Innes, and A P Greening Airways in cystic fibrosis are acidified: detection by exhaled breath condensate Thorax, November 1, 2002; 57(11): 926 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ishiguro, M.C. Steward, Y. Sohma, T. Kubota, M. Kitagawa, T. Kondo, R.M. Case, T. Hayakawa, and S. Naruse Membrane Potential and Bicarbonate Secretion in Isolated Interlobular Ducts from Guinea-pig Pancreas J. Gen. Physiol., October 29, 2002; 120(5): 617 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Xie, R. Welch, A. Mercado, M. F. Romero, and D. B. Mount Molecular characterization of the murine Slc26a6 anion exchanger: functional comparison with Slc26a1 Am J Physiol Renal Physiol, October 1, 2002; 283(4): F826 - F838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lohi, S. Makela, K. Pulkkinen, P. Hoglund, M.-L. Karjalainen-Lindsberg, P. Puolakkainen, and J. Kere Upregulation of CFTR expression but not SLC26A3 and SLC9A3 in ulcerative colitis Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G567 - G575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kulaksiz, E. Rehberg, W. Stremmel, and Y. Cetin Guanylin and Functional Coupling Proteins in the Human Salivary Glands and Gland Tumors : Expression, Cellular Localization, and Target Membrane Domains Am. J. Pathol., August 1, 2002; 161(2): 655 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nehrke, J. Arreola, H.-V. Nguyen, J. Pilato, L. Richardson, G. Okunade, R. Baggs, G. E. Shull, and J. E. Melvin Loss of Hyperpolarization-activated Cl- Current in Salivary Acinar Cells from Clcn2 Knockout Mice J. Biol. Chem., June 21, 2002; 277(26): 23604 - 23611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Poschet, J. Skidmore, J. C. Boucher, A. M. Firoved, R. W. Van Dyke, and V. Deretic Hyperacidification of Cellubrevin Endocytic Compartments and Defective Endosomal Recycling in Cystic Fibrosis Respiratory Epithelial Cells J. Biol. Chem., April 12, 2002; 277(16): 13959 - 13965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kunzelmann and M. Mall Electrolyte Transport in the Mammalian Colon: Mechanisms and Implications for Disease Physiol Rev, January 1, 2002; 82(1): 245 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Greeley, H. Shumaker, Z. Wang, C. W. Schweinfest, and M. Soleimani Downregulated in adenoma and putative anion transporter are regulated by CFTR in cultured pancreatic duct cells Am J Physiol Gastrointest Liver Physiol, November 1, 2001; 281(5): G1301 - G1308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Barriere, C. Poujeol, M. Tauc, J. M. Blasi, L. Counillon, and P. Poujeol CFTR modulates programmed cell death by decreasing intracellular pH in Chinese hamster lung fibroblasts Am J Physiol Cell Physiol, September 1, 2001; 281(3): C810 - C824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Chandy, M. Grabe, H.-P. H. Moore, and T. E. Machen Proton leak and CFTR in regulation of Golgi pH in respiratory epithelial cells Am J Physiol Cell Physiol, September 1, 2001; 281(3): C908 - C921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. J. Wheat, H. Shumaker, C. Burnham, G. E. Shull, J. R. Yankaskas, and M. Soleimani CFTR induces the expression of DRA along with Cl-/HCO3- exchange activity in tracheal epithelial cells Am J Physiol Cell Physiol, July 1, 2000; 279(1): C62 - C71. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Akabas Cystic Fibrosis Transmembrane Conductance Regulator. STRUCTURE AND FUNCTION OF AN EPITHELIAL CHLORIDE CHANNEL J. Biol. Chem., February 11, 2000; 275(6): 3729 - 3732. [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Widdicombe Yet Another Role for the Cystic Fibrosis Transmembrane Conductance Regulator Am. J. Respir. Cell Mol. Biol., January 1, 2000; 22(1): 11 - 14. [Full Text]
|
|
|
|
|
|
R. L. Evans, K. Park, R. J. Turner, G. E. Watson, H.-V. Nguyen, M. R. Dennett, A. R. Hand, M. Flagella, G. E. Shull, and J. E. Melvin Severe Impairment of Salivation in Na+/K+/2Cl- Cotransporter (NKCC1)-deficient Mice J. Biol. Chem., August 25, 2000; 275(35): 26720 - 26726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Luo, J. Y. Choi, S. B. H. Ko, A. Pushkin, I. Kurtz, W. Ahn, M. G. Lee, and S. Muallem HCO3- Salvage Mechanisms in the Submandibular Gland Acinar and Duct Cells J. Biol. Chem., March 23, 2001; 276(13): 9808 - 9816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Ahn, K. H. Kim, J. A. Lee, J. Y. Kim, J. Y. Choi, O. W. Moe, S. L. Milgram, S. Muallem, and M. G. Lee Regulatory Interaction between the Cystic Fibrosis Transmembrane Conductance Regulator and HCO3- Salvage Mechanisms in Model Systems and the Mouse Pancreatic Duct J. Biol. Chem., May 11, 2001; 276(20): 17236 - 17243. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
