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J Biol Chem, Vol. 274, Issue 21, 15073-15079, May 21, 1999

Phage 29 Terminal Protein Residues Asn⁸⁰ and Tyr⁸² Are Recognition Elements of the Replication Origins

From the Centro de Biología Molecular "Severo Ochoa" (Consejo Superior de Investigaciones Científicas-UAM), Universidad Autónoma, Canto Blanco, 28049 Madrid, Spain

Initiation of phage 29 DNA replication starts with the recognition of the origin of replication, located at both ends of the linear DNA, by a heterodimer formed by the 29 terminal protein (TP) and the 29 DNA polymerase. The parental TP, covalently linked to the DNA ends, is one of the main components of the replication origin. Here we provide evidence that recognition of the origin is mediated through interactions between the TP of the TP/DNA polymerase heterodimer, called primer TP, and the parental TP. Based on amino acid sequence comparisons, various 29 TP mutants were generated at conserved amino acid residues from positions 61 to 87. In vitro 29 DNA amplification analysis revealed that residues Asn⁸⁰ and Tyr⁸² are essential for functional interaction between primer and parental TP required for recognition of the origin of replication. Although these mutant TPs can form functional heterodimers with 29 DNA polymerase that are able to recognize the origin of replication, these heterodimers are not able to recognize an origin containing a mutant TP.

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