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J Biol Chem, Vol. 274, Issue 22, 15350-15359, May 28, 1999
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From the The kinetics of glucose transport and the
transcription of all 20 members of the HXT hexose
transporter gene family were studied in relation to the steady state
in situ carbon metabolism of Saccharomyces cerevisiae CEN.PK113-7D grown in chemostat cultures. Cells were cultivated at a dilution rate of 0.10 h E. C. Slater Institute, University of
Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands, the § Kluyver
Laboratory of Biotechnology, Delft University of Technology,
Julianalaan 67, 2628 BC Delft, The Netherlands,
¶ Gist-brocades B. V., PO Box 1, 2600 MA Delft, The Netherlands, and the ** Department of
Chemical Engineering, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
1 under various
nutrient-limited conditions (anaerobically glucose- or nitrogen-limited
or aerobically glucose-, galactose-, fructose-, ethanol-, or
nitrogen-limited), or at dilution rates ranging between 0.05 and 0.38 h1 in aerobic glucose-limited cultures. Transcription of
HXT1-HXT7 was correlated with the extracellular glucose
concentration in the cultures. Transcription of GAL2,
encoding the galactose transporter, was only detected in
galactose-limited cultures. SNF3 and RGT2, two
members of the HXT family that encode glucose sensors, were transcribed at low levels. HXT8-HXT17 transcripts were
detected at very low levels. A consistent relationship was observed
between the expression of individual HXT genes and the
glucose transport kinetics determined from zero-trans
influx of 14C-glucose during 5 s. This relationship
was in broad agreement with the transport kinetics of Hxt1-Hxt7 and
Gal2 deduced in previous studies on single-HXT strains. At
lower dilution rates the glucose transport capacity estimated from
zero-trans influx experiments and the residual glucose
concentration exceeded the measured in situ glucose
consumption rate. At high dilution rates, however, the estimated
glucose transport capacity was too low to account for the in
situ glucose consumption rate.
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