HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Allen, R. M. Articles by Ludden, P. W. Search for Related Content PubMed PubMed Citation Articles by Allen, R. M. Articles by Ludden, P. W. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 22, 15869-15874, May 28, 1999

Incorporation of Molybdenum into the Iron-Molybdenum Cofactor of Nitrogenase

Ronda M. Allen^§, Jon T. Roll^§, Priya Rangaraj^§, Vinod K. Shah^§, Gary P. Roberts^§, and Paul W. Ludden^§

From the Departments of  Biochemistry and  Bacteriology and the ^§ Center for the Study of Nitrogen Fixation, University of Wisconsin, Madison, Wisconsin 53706

The biosynthesis of the iron-molybdenum cofactor (FeMo-co) of dinitrogenase was investigated using ⁹⁹Mo to follow the incorporation of Mo into precursors. ⁹⁹Mo label accumulates on dinitrogenase only when all known components of the FeMo-co synthesis system, NifH, NifNE, NifB-cofactor, homocitrate, MgATP, and reductant, are present. Furthermore, ⁹⁹Mo label accumulates only on the gamma protein, which has been shown to serve as a chaperone/insertase for the maturation of apodinitrogenase when all known components are present. It appears that only completed FeMo-co can accumulate on the gamma protein. Very little FeMo-co synthesis was observed when all known components are used in purified forms, indicating that additional factors are required for optimal FeMo-co synthesis. ⁹⁹Mo did not accumulate on NifNE under any conditions tested, suggesting that Mo enters the pathway at some other step, although it remains possible that a Mo-containing precursor of FeMo-co that is not sufficiently stable to persist during gel electrophoresis occurs but is not observed. ⁹⁹Mo accumulates on several unidentified species, which may be the additional components required for FeMo-co synthesis. The molybdenum storage protein was observed and the accumulation of ⁹⁹Mo on this protein required nucleotide.

---

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				B. Soboh, R. Y. Igarashi, J. A. Hernandez, and L. M. Rubio Purification of a NifEN Protein Complex That Contains Bound Molybdenum and a FeMo-Co Precursor from an Azotobacter vinelandii {Delta}nifHDK Strain J. Biol. Chem., December 1, 2006; 281(48): 36701 - 36709. [Abstract] [Full Text] [PDF]

				L. M. Rubio, S. W. Singer, and P. W. Ludden Purification and Characterization of NafY (Apodinitrogenase {gamma} Subunit) from Azotobacter vinelandii J. Biol. Chem., May 7, 2004; 279(19): 19739 - 19746. [Abstract] [Full Text] [PDF]

				P. Rangaraj and P. W. Ludden Accumulation of 99Mo-containing Iron-Molybdenum Cofactor Precursors of Nitrogenase on NifNE, NifH, and NifX of Azotobacter vinelandii J. Biol. Chem., October 11, 2002; 277(42): 40106 - 40111. [Abstract] [Full Text] [PDF]

				P. Rangaraj, C. Ruttimann-Johnson, V. K. Shah, and P. W. Ludden Accumulation of 55Fe-Labeled Precursors of the Iron-Molybdenum Cofactor of Nitrogenase on NifH and NifX of Azotobacter vinelandii J. Biol. Chem., May 4, 2001; 276(19): 15968 - 15974. [Abstract] [Full Text] [PDF]

				K. Makdessi, J. R. Andreesen, and A. Pich Tungstate Uptake by a Highly Specific ABC Transporter in Eubacterium acidaminophilum J. Biol. Chem., June 29, 2001; 276(27): 24557 - 24564. [Abstract] [Full Text] [PDF]