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J Biol Chem, Vol. 274, Issue 24, 16825-16830, June 11, 1999
From the Department of Pharmaceutical Sciences, University of
Maryland School of Pharmacy, Baltimore, Maryland 21201-1180 and the
Program of Oncology, Marlene and Stewart Greenebaum Cancer Center
of the University of Maryland, Baltimore, Maryland 21201-1180
Adipose differentiation related protein (ADRP) is
a 50-kDa novel protein cloned from a mouse 1246 adipocyte cDNA
library, rapidly induced during adipocyte differentiation. We have
examined ADRP function, and we show here that ADRP facilitates fatty
acid uptake in COS cells transfected with ADRP cDNA. We demonstrate that uptake of long chain fatty acids was significantly stimulated in a
time-dependent fashion in ADRP-expressing COS-7 cells
compared with empty vector-transfected control cells. Oleic acid uptake velocity increased significantly in a dose-dependent manner
in ADRP-expressing COS-7 cells compared with control cells. The
transport Km was 0.051 µM, and
Vmax was 57.97 pmol/105 cells/min
in ADRP-expressing cells, and Km was 0.093 µM and Vmax was 20.13 pmol/105 cells/min in control cells. The oleate uptake
measured at 4 °C was only 10% that at 37 °C. ADRP also
stimulated uptake of palmitate and arachidonate but had no effect on
uptake of medium chain fatty acid such as octanoic acid and glucose.
These data suggest that ADRP specifically enhances uptake of long chain
fatty acids by increasing the initial rate of uptake and provide novel
information about ADRP function as a saturable transport component for
long chain fatty acids.
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