HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Joester, A. Articles by Faissner, A. Search for Related Content PubMed PubMed Citation Articles by Joester, A. Articles by Faissner, A. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 24, 17144-17151, June 11, 1999

Evidence for Combinatorial Variability of Tenascin-C Isoforms and Developmental Regulation in the Mouse Central Nervous System

Angret Joester and Andreas Faissner^§

From the  Department of Neurobiology, University of Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany and ^§ Laboratoire de Neurobiologie du Développement et de la Régénération, UPR 1352, Centre de Neurochimie du CNRS et Université Louis Pasteur, F-67084 Strasbourg, France

The extracellular matrix glycoprotein tenascin-C (TN-C) displays a restricted and developmentally regulated distribution in the mouse central nervous system. Defined modules of the molecule have been shown to mediate specific functions, such as neuron migration, neurite outgrowth, cell adhesion, and cell proliferation. The smallest TN-C form contains a stretch of eight fibronectin type III (FNIII) domains, which are common to all TN-C isoforms. Unrestricted and independent alternative splicing of six consecutive FNIII cassettes between the fifth and sixth constitutive FNIII domain bears the potential to generate 64 different combinations that might code for TN-C proteins with subtly different functions. To explore TN-C isoform variability in mouse brain, the alternatively spliced region of TN-C mRNAs was examined by the reverse transcription-polymerase chain reaction technique. Polymerase chain reaction products of uniform size were subcloned and analyzed using domain-specific probes to reveal the expression of particular combinations of alternatively spliced FNIII domains. 27 TN-C isoforms were identified to be expressed in mouse central nervous system, of which 22 are novel. Furthermore, during development, specific TN-C isoforms were found to occur in distinct relative frequencies, as demonstrated for isoforms containing two alternatively spliced FNIII domains. We conclude that TN-C is expressed in a complex and regulated pattern in mouse central nervous system. These findings highlight the potential role of TN-C in mediating specific neuron glia interactions.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				S. Moritz, S. Lehmann, A. Faissner, and A. von Holst An Induction Gene Trap Screen in Neural Stem Cells Reveals an Instructive Function of the Niche and Identifies the Splicing Regulator Sam68 as a Tenascin-C-Regulated Target Gene Stem Cells, September 1, 2008; 26(9): 2321 - 2331. [Abstract] [Full Text] [PDF]

				A. von Holst, U. Egbers, A. Prochiantz, and A. Faissner Neural Stem/Progenitor Cells Express 20 Tenascin C Isoforms That Are Differentially Regulated by Pax6 J. Biol. Chem., March 23, 2007; 282(12): 9172 - 9181. [Abstract] [Full Text] [PDF]

				K. Sugaya, E. Hongo, Y. Ishihara, and H. Tsuji The conserved role of Smu1 in splicing is characterized in its mammalian temperature-sensitive mutant J. Cell Sci., December 1, 2006; 119(23): 4944 - 4951. [Abstract] [Full Text] [PDF]

				H. C. Hsia and J. E. Schwarzbauer Meet the Tenascins: Multifunctional and Mysterious J. Biol. Chem., July 22, 2005; 280(29): 26641 - 26644. [Full Text] [PDF]

				M. L. T. Mercado, A. Nur-e-Kamal, H.-Y. Liu, S. R. Gross, R. Movahed, and S. Meiners Neurite Outgrowth by the Alternatively Spliced Region of Human Tenascin-C Is Mediated by Neuronal {alpha}7{beta}1 Integrin J. Neurosci., January 7, 2004; 24(1): 238 - 247. [Abstract] [Full Text] [PDF]

				T. Tsunoda, H. Inada, I. Kalembeyi, K. Imanaka-Yoshida, M. Sakakibara, R. Okada, K. Katsuta, T. Sakakura, Y. Majima, and T. Yoshida Involvement of Large Tenascin-C Splice Variants in Breast Cancer Progression Am. J. Pathol., June 1, 2003; 162(6): 1857 - 1867. [Abstract] [Full Text] [PDF]

				F. Rigato, J. Garwood, V. Calco, N. Heck, C. Faivre-Sarrailh, and A. Faissner Tenascin-C Promotes Neurite Outgrowth of Embryonic Hippocampal Neurons through the Alternatively Spliced Fibronectin Type III BD Domains via Activation of the Cell Adhesion Molecule F3/Contactin J. Neurosci., August 1, 2002; 22(15): 6596 - 6609. [Abstract] [Full Text] [PDF]

				M. Adams, J. L. Jones, R. A. Walker, J. H. Pringle, and S. C. Bell Changes in Tenascin-C Isoform Expression in Invasive and Preinvasive Breast Disease Cancer Res., June 1, 2002; 62(11): 3289 - 3297. [Abstract] [Full Text] [PDF]

				A. V. Ljubimov, M. Saghizadeh, R. Pytela, D. Sheppard, and M. C. Kenney Increased Expression of Tenascin-C-binding Epithelial Integrins in Human Bullous Keratopathy Corneas J. Histochem. Cytochem., November 1, 2001; 49(11): 1341 - 1350. [Abstract] [Full Text] [PDF]

				E. Garcion, A. Faissner, and C. ffrench-Constant Knockout mice reveal a contribution of the extracellular matrix molecule tenascin-C to neural precursor proliferation and migration Development, July 1, 2001; 128(13): 2485 - 2496. [Abstract] [Full Text] [PDF]

				S. Meiners, M. L. T. Mercado, M. S. A. Nur-e-Kamal, and H. M. Geller Tenascin-C Contains Domains That Independently Regulate Neurite Outgrowth and Neurite Guidance J. Neurosci., October 1, 1999; 19(19): 8443 - 8453. [Abstract] [Full Text] [PDF]