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J Biol Chem, Vol. 274, Issue 24, 17379-17383, June 11, 1999
From the Department of Biochemistry, Lundberg Institute, Chalmers
University of Technology, Box 462, SE 405 30 Göteborg,
Sweden
It is emerging that DNA tetraplexes are pivotal
for many major cellular processes, and techniques that assess their
structure and nature to the point are under development. Here we show
how the structural conversion of largely unstructured single-stranded DNA molecules into compact intrastrand DNA tetraplexes can be monitored
by fluorescence resonance energy transfer.
We recently reported that intrastrand tetraplex formation takes place
in a nuclease hypersensitive element upstream of the human
c-myc proto-oncogene. Despite the highly repetitive
guanine-rich sequence of the hypersensitive element, fluorescence
resonance energy transfer measurements indicate that only one well
defined tetraplex structure forms therein. The proposed structure,
which is specifically stabilized by potassium ions in
vitro, has a core of three stacked guanine tetrads that is capped
by two intrastrand A-T base pairs.
DNA Tetraplex Formation Studied with Fluorescence Resonance
Energy Transfer
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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