| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 25, 17671-17676, June 18, 1999
From the College of Physicians and Surgeons of Columbia University,
New York, New York 10032
When an intercalated epithelial cell line was
seeded at low density and allowed to reach confluence, it located the
anion exchanger band 3 in the apical membrane and an
H+-ATPase in the basolateral membrane. The same
clonal cells seeded at high density targeted these proteins to the
reverse location. Furthermore, high density cells had vigorous apical
endocytosis, and low density cells had none. The extracellular matrix
of high density cells was capable of inducing apical endocytosis and
relocation of band 3 to the basolateral membrane in low density cells.
A 230-kDa extracellular matrix (ECM) protein termed hensin, when purified to near-homogeneity, was able to reverse the phenotype of the
low density cells. Antibodies to hensin prevented this effect,
indicating that hensin is necessary for conversion of polarity. We show
here that hensin was synthesized by both low density and high density
cells. Whereas both phenotypes secreted soluble hensin into their
media, only high density cells localized it in their ECM. Analysis of
soluble hensin by sucrose density gradients showed that low density
cells secreted monomeric hensin, and high density cells secreted higher
order multimers. When 35S-labeled monomeric hensin was
added to high density cells, they induced its aggregation suggesting
that the multimerization was catalyzed by surface events in the high
density cells. Soluble monomeric or multimeric hensin did not induce
apical endocytosis in low density cells, whereas the more polymerized
hensin isolated from insoluble ECM readily induced it. These multimers
could be disaggregated by sulfhydryl reagents and by dimethylmaleic
anhydride, and treatment of high density ECM by these reagents
prevented the induction of endocytosis. These results demonstrate that
hensin, like several ECM proteins, needs to be precipitated in the ECM to be functional.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Vijayakumar, H. Erdjument-Bromage, P. Tempst, and Q. Al-Awqati Role of Integrins in the Assembly and Function of Hensin in Intercalated Cells J. Am. Soc. Nephrol., June 1, 2008; 19(6): 1079 - 1091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Al-Awqati 2007 Homer W. Smith Award: Control of Terminal Differentiation in Epithelia J. Am. Soc. Nephrol., March 1, 2008; 19(3): 443 - 449. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Vijayakumar, J. Takito, X. Gao, G. J. Schwartz, and Q. Al-Awqati Differentiation of columnar epithelia: the hensin pathway J. Cell Sci., December 1, 2006; 119(23): 4797 - 4801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Schwaderer, S. Vijayakumar, Q. Al-Awqati, and G. J. Schwartz Galectin-3 expression is induced in renal {beta}-intercalated cells during metabolic acidosis Am J Physiol Renal Physiol, January 1, 2006; 290(1): F148 - F158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Watanabe, S. Tsuruoka, S. Vijayakumar, G. Fischer, Y. Zhang, A. Fujimura, Q. Al-Awqati, and G. J. Schwartz Cyclosporin A produces distal renal tubular acidosis by blocking peptidyl prolyl cis-trans isomerase activity of cyclophilin Am J Physiol Renal Physiol, January 1, 2005; 288(1): F40 - F47. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Takito and Q. Al-Awqati Conversion of ES cells to columnar epithelia by hensin and to squamous epithelia by laminin J. Cell Biol., September 27, 2004; 166(7): 1093 - 1102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Al-Awqati, S. Vijayakumar, and J. Takito Terminal Differentiation of Epithelia from Trophectoderm to the Intercalated Cell: The Role of Hensin J. Am. Soc. Nephrol., June 1, 2003; 14(90001): S16 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Hikita, S. Vijayakumar, J. Takito, H. Erdjument-Bromage, P. Tempst, and Q. Al-Awqati Induction of Terminal Differentiation in Epithelial Cells Requires Polymerization of Hensin by Galectin 3 J. Cell Biol., December 11, 2000; 151(6): 1235 - 1246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Mollenhauer, S. Herbertz, U. Holmskov, M. Tolnay, I. Krebs, A. Merlo, H. D. Schrøder, D. Maier, F. Breitling, S. Wiemann, et al. DMBT1 Encodes a Protein Involved in the Immune Defense and in Epithelial Differentiation and Is Highly Unstable in Cancer Cancer Res., March 1, 2000; 60(6): 1704 - 1710. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
