| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 25, 17742-17747, June 18, 1999
From the To identify potential proteins interacting with
the insulin-responsive glucose transporter (GLUT4), we generated fusion
proteins of glutathione S-transferase (GST) and the final
30 amino acids from GLUT4 (GST-G4) or GLUT1 (GST-G1). Incubation of
these carboxyl-terminal fusion proteins with adipocyte cell extracts
revealed a specific interaction of GLUT4 with fructose 1,6-bisphosphate
aldolase. In the presence of aldolase, GST-G4 but not GST-G1 was able
to co-pellet with filamentous (F)-actin. This interaction was prevented by incubation with the aldolase substrates, fructose 1,6-bisphosphate or glyceraldehyde 3-phosphate. Immunofluorescence confocal microscopy demonstrated a significant co-localization of aldolase and GLUT4 in
intact 3T3L1 adipocytes, which decreased following insulin stimulation.
Introduction into permeabilized 3T3L1 adipocytes of fructose
1,6-bisphosphate or the metabolic inhibitor 2-deoxyglucose, two agents
that disrupt the interaction between aldolase and actin, inhibited
insulin-stimulated GLUT4 exocytosis without affecting GLUT4
endocytosis. Furthermore, microinjection of an aldolase-specific antibody also inhibited insulin-stimulated GLUT4 translocation. These
data suggest that aldolase functions as a scaffolding protein for GLUT4
and that glucose metabolism may provide a negative feedback signal for
the regulation of glucose transport by insulin.
Aldolase Mediates the Association of F-actin with the
Insulin-responsive Glucose Transporter GLUT4
,, and Department of Physiology and Biophysics,
University of Iowa, Iowa City, Iowa 52242, ¶ Department of Cell
Biology, Parke-Davis Pharmaceutical Research Division, Ann Arbor,
Michigan 48105, and the Department of Physiology, University of
Michigan School of Medicine, Ann Arbor, Michigan 48109
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Benziane, S. Demaretz, N. Defontaine, N. Zaarour, L. Cheval, S. Bourgeois, C. Klein, M. Froissart, A. Blanchard, M. Paillard, et al. NKCC2 Surface Expression in Mammalian Cells: DOWN-REGULATION BY NOVEL INTERACTION WITH ALDOLASE B J. Biol. Chem., November 16, 2007; 282(46): 33817 - 33830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Diaz, C. N. Antonescu, E. Capilla, A. Klip, and J. V. Planas Fish Glucose Transporter (GLUT)-4 Differs from Rat GLUT4 in Its Traffic Characteristics but Can Translocate to the Cell Surface in Response to Insulin in Skeletal Muscle Cells Endocrinology, November 1, 2007; 148(11): 5248 - 5257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. St-Jean, T. Izard, and J. Sygusch A Hydrophobic Pocket in the Active Site of Glycolytic Aldolase Mediates Interactions with Wiskott-Aldrich Syndrome Protein J. Biol. Chem., May 11, 2007; 282(19): 14309 - 14315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Buscaglia, D. Penesetti, M. Tao, and V. Nussenzweig Characterization of an Aldolase-binding Site in the Wiskott-Aldrich Syndrome Protein J. Biol. Chem., January 20, 2006; 281(3): 1324 - 1331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ishiki and A. Klip Minireview: Recent Developments in the Regulation of Glucose Transporter-4 Traffic: New Signals, Locations, and Partners Endocrinology, December 1, 2005; 146(12): 5071 - 5078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ishiki, V. K. Randhawa, V. Poon, L. JeBailey, and A. Klip Insulin Regulates the Membrane Arrival, Fusion, and C-terminal Unmasking of Glucose Transporter-4 via Distinct Phosphoinositides J. Biol. Chem., August 5, 2005; 280(31): 28792 - 28802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Canete-Soler, K. S. Reddy, D. R. Tolan, and J. Zhai Aldolases A and C Are Ribonucleolytic Components of a Neuronal Complex That Regulates the Stability of the Light-Neurofilament mRNA J. Neurosci., April 27, 2005; 25(17): 4353 - 4364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Arakaki, J. A. Pezza, M. A. Cronin, C. E. Hopkins, D. B. Zimmer, D. R. Tolan, and K. N. Allen Structure of human brain fructose 1,6-(bis)phosphate aldolase: Linking isozyme structure with function Protein Sci., December 1, 2004; 13(12): 3077 - 3084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Lundmark and S. R. Carlsson Regulated Membrane Recruitment of Dynamin-2 Mediated by Sorting Nexin 9 J. Biol. Chem., October 8, 2004; 279(41): 42694 - 42702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Vallejo and C. D. Hardin Metabolic organization in vascular smooth muscle: distribution and localization of caveolin-1 and phosphofructokinase Am J Physiol Cell Physiol, January 1, 2004; 286(1): C43 - C54. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Buscaglia, I. Coppens, W. G. J. Hol, and V. Nussenzweig Sites of Interaction between Aldolase and Thrombospondin-related Anonymous Protein in Plasmodium Mol. Biol. Cell, December 1, 2003; 14(12): 4947 - 4957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tojo, I. Kaieda, H. Hattori, N. Katayama, K. Yoshimura, S. Kakimoto, Y. Fujisawa, E. Presman, C. C. Brooks, and P. F. Pilch The Formin Family Protein, Formin Homolog Overexpressed in Spleen, Interacts with the Insulin-Responsive Aminopeptidase and Profilin IIa Mol. Endocrinol., July 1, 2003; 17(7): 1216 - 1229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Pezza, K. H. Choi, T. Z. Berardini, P. T. Beernink, K. N. Allen, and D. R. Tolan Spatial Clustering of Isozyme-specific Residues Reveals Unlikely Determinants of Isozyme Specificity in Fructose-1,6-bisphosphate Aldolase J. Biol. Chem., May 2, 2003; 278(19): 17307 - 17313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Gartzke and K. Lange Cellular target of weak magnetic fields: ionic conduction along actin filaments of microvilli Am J Physiol Cell Physiol, November 1, 2002; 283(5): C1333 - C1346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Lange Role of microvillar cell surfaces in the regulation of glucose uptake and organization of energy metabolism Am J Physiol Cell Physiol, January 1, 2002; 282(1): C1 - C26. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang, A. Xu, J. Ye, E. W. Kraegen, C. A. Tse, and G. J.S. Cooper Alteration in Phosphorylation of P20 Is Associated With Insulin Resistance Diabetes, August 1, 2001; 50(8): 1821 - 1827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Patki, J. Buxton, A. Chawla, L. Lifshitz, K. Fogarty, W. Carrington, R. Tuft, and S. Corvera Insulin Action on GLUT4 Traffic Visualized in Single 3T3-L1 Adipocytes by Using Ultra-fast Microscopy Mol. Biol. Cell, January 1, 2001; 12(1): 129 - 141. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. Giorgino, O. de Robertis, L. Laviola, C. Montrone, S. Perrini, K. C. McCowen, and R. J. Smith The sentrin-conjugating enzyme mUbc9 interacts with GLUT4 and GLUT1 glucose transporters and regulates transporter levels in skeletal muscle cells PNAS, February 1, 2000; 97(3): 1125 - 1130. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
