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J Biol Chem, Vol. 274, Issue 25, 17893-17900, June 18, 1999
From the Department of Cell and Tumor Biology, City of Hope
National Medical Center and Beckman Research Institute, Duarte,
California 91010 and the § Division of Biology, California
Institute of Technology, Pasadena, California 91125
Yeast exonuclease 1 (Exo1) is induced during
meiosis and plays an important role in DNA homologous recombination and
mismatch correction pathways. The human homolog, an 803-amino acid
protein, shares 55% similarity to the yeast Exo1. In this report, we
show that the enzyme functionally complements Saccharomyces
cerevisiae Exo1 in recombination of direct repeat DNA fragments,
UV resistance, and mutation avoidance by in vivo assays.
Furthermore, the human enzyme suppresses the conditional lethality of a
rad27
Human Exonuclease 1 Functionally Complements Its Yeast Homologues
in DNA Recombination, RNA Primer Removal, and Mutation
Avoidance
mutant, symptomatic of defective RNA primer removal. The
purified recombinant enzyme not only displays 5'-3' double strand DNA
exonuclease activity, but also shows an RNase H activity. This result
indicates a back-up function of exonuclease 1 to flap endonuclease-1 in
RNA primer removal during lagging strand DNA synthesis.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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