| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 25, 17924-17933, June 18, 1999
From the The autonomous replication region of
plasmid ColIb-P9 contains repZ encoding the RepZ
replication protein, and inc and repY as the
negative and positive regulators of repZ translation,
respectively. inc encodes the antisense Inc RNA, and
repY is a short open reading frame upstream of
repZ. Translation of repY enables
repZ translation by inducing formation of a pseudoknot
containing stem-loop I, which base pairs with the sequence preceding
the repZ start codon. Inc RNA inhibits both
repY translation and formation of the pseudoknot by binding
to the loop I. To investigate control of repY expression by
Inc RNA, we isolated a number of mutations that express
repY in the presence of Inc RNA. One class of mutations
delete a part of another stem-loop (II), which derepresses
repY expression by initiating translation at codon 10 (GUG), located within this structure. Point mutations in stem-loop II
can also derepress repY translation, and the introduction
of compensatory base-changes restores control of repY
translation. These results not only indicate that suppressing a cryptic
start codon by secondary structure is important for maintaining the
translational control of repZ but also demonstrate that the
position of start site for repY translation is critical for
its control by Inc RNA. Thus, Inc RNA controls repY
translation by binding in the vicinity of the start codon, in contrast
to the control of repZ expression at the level of loop-loop interaction.
The Plasmid ColIb-P9 Antisense Inc RNA Controls Expression of the
RepZ Replication Protein and Its Positive Regulator repY
with Different Mechanisms
,,,, and
Department of Biophysics and Biochemistry,
Graduate School of Science, University of Tokyo, Hongo,
Tokyo 113, Japan and the Department of Applied Physics and
Chemistry, University of Electro-Communications, Chofu-shi,
Tokyo 182, Japan
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Betteridge, J. Yang, A. J. Pittard, and J. Praszkier Interaction of the Initiator Protein of an IncB Plasmid with Its Origin of DNA Replication J. Bacteriol., April 1, 2003; 185(7): 2210 - 2218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gottesman Stealth regulation: biological circuits with small RNA switches Genes & Dev., November 15, 2002; 16(22): 2829 - 2842. [Full Text] [PDF]
|
|
|
|
|
|
J. Praszkier and A. J. Pittard Pseudoknot-Dependent Translational Coupling in repBA Genes of the IncB Plasmid pMU720 Involves Reinitiation J. Bacteriol., October 15, 2002; 184(20): 5772 - 5780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-Y. Wu, J.-F. Fu, and S.-T. Liu The replicon of pSW800 from Pantoea stewartii Microbiology, October 1, 2001; 147(10): 2757 - 2767. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. Kolb, E. Westhof, C. Ehresmann, B. Ehresmann, E. G. H. Wagner, and P. Romby Bulged residues promote the progression of a loop-loop interaction to a stable and inhibitory antisense-target RNA complex Nucleic Acids Res., August 1, 2001; 29(15): 3145 - 3153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Asano and K. Mizobuchi Structural Analysis of Late Intermediate Complex Formed between Plasmid ColIb-P9 Inc RNA and Its Target RNA. HOW DOES A SINGLE ANTISENSE RNA REPRESS TRANSLATION OF TWO GENES AT DIFFERENT RATES? J. Biol. Chem., January 14, 2000; 275(2): 1269 - 1274. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
