JBC Advanced Peptides, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tsukamoto, N.
Right arrow Articles by Minato, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsukamoto, N.
Right arrow Articles by Minato, N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 274, Issue 26, 18463-18469, June 25, 1999

Rap1 GTPase-activating Protein SPA-1 Negatively Regulates Cell Adhesion

Noriyuki Tsukamoto, Masakazu Hattori, Hailin Yang, Johannes L. BosDagger , and Nagahiro Minato

From the Department of Immunology and Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan and the Dagger  Laboratory for Physiological Chemistry, Utrecht University, 2584 CG Utrecht, The Netherlands

Rap1 GTPase is activated by a variety of stimulations in many types of cells, but its exact functions remain unknown. In this study we have shown that SPA-1 interferes with Rap1 activation by membrane-targeted C3G, C3G-F, in 293T cells through the GTPase activating protein (GAP) activity. SPA-1 transiently expressed in HeLa cells was mostly localized at the cortical cytoskeleton and induced rounding up of the cells, whereas C3G-F conversely induced extensive cell spreading. Conditional SPA-1 overexpression in HeLa cells by tetracycline-regulative system suppressed Rap1 activation upon plating on dishes coated with fibronectin and resulted in the reduced adhesion. When SPA-1 was conditionally induced after the established cell adhesion, the cells gradually rounded up and detached from the dish. Both effects were counteracted by exogenous fibronectin in a dose-dependent manner. Retroviral overexpression of SPA-1 in promyelocytic 32D cells also inhibited both activation of Rap1 and induction of cell adhesion by granulocyte colony stimulating factor without affecting differentiation. These results have indicated that Rap1 GTP is required for the cell adhesion induced by both extracellular matrix and soluble factors, which is negatively regulated by SPA-1.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 BloodHome page
K. Kometani, M. Moriyama, Y. Nakashima, Y. Katayama, S.-F. Wang, S. Yamasaki, T. Saito, M. Hattori, and N. Minato
Essential role of Rap signal in pre-TCR-mediated {beta}-selection checkpoint in {alpha}{beta} T-cell development
Blood, December 1, 2008; 112(12): 4565 - 4573.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Chu, A. Awasthi, G. C. White II, M. Chrzanowska-Wodnicka, and S. Malarkannan
Rap1b Regulates B Cell Development, Homing, and T Cell-Dependent Humoral Immunity
J. Immunol., September 1, 2008; 181(5): 3373 - 3383.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S.-F. Wang, M. Aoki, Y. Nakashima, Y. Shinozuka, H. Tanaka, M. Taniwaki, M. Hattori, and N. Minato
Development of Notch-dependent T-cell leukemia by deregulated Rap1 signaling
Blood, March 1, 2008; 111(5): 2878 - 2886.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
A. A.A. Ewies, M. Elshafie, J. Li, A. Stanley, J. Thompson, J. Styles, I. White, and F. Al-Azzawi
Changes in transcription profile and cytoskeleton morphology in pelvic ligament fibroblasts in response to stretch: the effects of estradiol and levormeloxifene
Mol. Hum. Reprod., February 1, 2008; 14(2): 127 - 135.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. Richter, K. K. Murai, C. Bourgin, D. T. Pak, and E. B. Pasquale
The EphA4 Receptor Regulates Neuronal Morphology through SPAR-Mediated Inactivation of Rap GTPases
J. Neurosci., December 19, 2007; 27(51): 14205 - 14215.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. Ghandour, X. Cullere, A. Alvarez, F. W. Luscinskas, and T. N. Mayadas
Essential role for Rap1 GTPase and its guanine exchange factor CalDAG-GEFI in LFA-1 but not VLA-4 integrin mediated human T-cell adhesion
Blood, November 15, 2007; 110(10): 3682 - 3690.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Yoshikawa, T. Satoh, T. Tamura, P. Wei, S. E. Bilasy, H. Edamatsu, A. Aiba, K. Katagiri, T. Kinashi, K. Nakao, et al.
The M-Ras-RA-GEF-2-Rap1 Pathway Mediates Tumor Necrosis Factor-{alpha} dependent Regulation of Integrin Activation in Splenocytes
Mol. Biol. Cell, August 1, 2007; 18(8): 2949 - 2959.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Mazzochi, D. J. Benos, and P. R. Smith
Interaction of epithelial ion channels with the actin-based cytoskeleton
Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1113 - F1122.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. P. Regalado, R. T. Terry-Lorenzo, C. L. Waites, C. C. Garner, and R. C. Malenka
Transsynaptic signaling by postsynaptic synapse-associated protein 97.
J. Neurosci., February 22, 2006; 26(8): 2343 - 2357.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. Goichberg, A. Kalinkovich, N. Borodovsky, M. Tesio, I. Petit, A. Nagler, I. Hardan, and T. Lapidot
cAMP-induced PKC{zeta} activation increases functional CXCR4 expression on human CD34+ hematopoietic progenitors
Blood, February 1, 2006; 107(3): 870 - 879.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Duchniewicz, T. Zemojtel, M. Kolanczyk, S. Grossmann, J. S. Scheele, and F. J. T. Zwartkruis
Rap1A-Deficient T and B Cells Show Impaired Integrin-Mediated Cell Adhesion
Mol. Cell. Biol., January 15, 2006; 26(2): 643 - 653.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. J. S. Stork and T. J. Dillon
Multiple roles of Rap1 in hematopoietic cells: complementary versus antagonistic functions
Blood, November 1, 2005; 106(9): 2952 - 2961.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Murata, M. Hattori, N. Hirai, Y. Shinozuka, H. Hirata, R. Kageyama, T. Sakai, and N. Minato
Hes1 Directly Controls Cell Proliferation through the Transcriptional Repression of p27Kip1
Mol. Cell. Biol., May 15, 2005; 25(10): 4262 - 4271.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
W. P.-v. Berkel, M.H.G. Verheijen, E. Cuppen, M. Asahina, J. de Rooij, G. Jansen, R.H.A. Plasterk, J. L. Bos, and F.J.T. Zwartkruis
Requirement of the Caenorhabditis elegans RapGEF pxf-1 and rap-1 for Epithelial Integrity
Mol. Biol. Cell, January 1, 2005; 16(1): 106 - 116.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Enserink, L. S. Price, T. Methi, M. Mahic, A. Sonnenberg, J. L. Bos, and K. Tasken
The cAMP-Epac-Rap1 Pathway Regulates Cell Spreading and Cell Adhesion to Laminin-5 through the {alpha}3{beta}1 Integrin but Not the {alpha}6{beta}4 Integrin
J. Biol. Chem., October 22, 2004; 279(43): 44889 - 44896.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Farina, M. Hattori, J. Qin, Y. Nakatani, N. Minato, and K. Ozato
Bromodomain Protein Brd4 Binds to GTPase-Activating SPA-1, Modulating Its Activity and Subcellular Localization
Mol. Cell. Biol., October 15, 2004; 24(20): 9059 - 9069.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. J. Ralston, S. L. Hird, X. Zhang, J. L. Scott, B. Jin, R. F. Thorne, M. C. Berndt, A. W. Boyd, and G. F. Burns
The LFA-1-associated Molecule PTA-1 (CD226) on T Cells Forms a Dynamic Molecular Complex with Protein 4.1G and Human Discs Large
J. Biol. Chem., August 6, 2004; 279(32): 33816 - 33828.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. Kinashi, M. Aker, M. Sokolovsky-Eisenberg, V. Grabovsky, C. Tanaka, R. Shamri, S. Feigelson, A. Etzioni, and R. Alon
LAD-III, a leukocyte adhesion deficiency syndrome associated with defective Rap1 activation and impaired stabilization of integrin bonds
Blood, February 1, 2004; 103(3): 1033 - 1036.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
M. Hattori and N. Minato
Rap1 GTPase: Functions, Regulation, and Malignancy
J. Biochem., October 1, 2003; 134(4): 479 - 484.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Ishida, H. Yang, K. Masuda, K. Uesugi, H. Kawamoto, M. Hattori, and N. Minato
Antigen-driven T cell anergy and defective memory T cell response via deregulated Rap1 activation in SPA-1-deficient mice
PNAS, September 16, 2003; 100(19): 10919 - 10924.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
B. Boettner, P. Harjes, S. Ishimaru, M. Heke, H. Q. Fan, Y. Qin, L. Van Aelst, and U. Gaul
The AF-6 Homolog Canoe Acts as a Rap1 Effector During Dorsal Closure of the Drosophila Embryo
Genetics, September 1, 2003; 165(1): 159 - 169.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Su, M. Hattori, M. Moriyama, N. Murata, M. Harazaki, K. Kaibuchi, and N. Minato
AF-6 Controls Integrin-mediated Cell Adhesion by Regulating Rap1 Activation through the Specific Recruitment of Rap1GTP and SPA-1
J. Biol. Chem., April 18, 2003; 278(17): 15232 - 15238.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
D. A. Marchuk, S. Srinivasan, T. L. Squire, and J. S. Zawistowski
Vascular morphogenesis: tales of two syndromes
Hum. Mol. Genet., April 2, 2003; 12(90001): R97 - 112.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Rangarajan, J. M. Enserink, H. B. Kuiperij, J. de Rooij, L. S. Price, F. Schwede, and J. L. Bos
Cyclic AMP induces integrin-mediated cell adhesion through Epac and Rap1 upon stimulation of the {beta}2-adrenergic receptor
J. Cell Biol., February 18, 2003; 160(4): 487 - 493.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Caron
Cellular functions of the Rap1 GTP-binding protein: a pattern emerges
J. Cell Sci., February 1, 2003; 116(3): 435 - 440.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L. Singh, Q. Gao, A. Kumar, T. Gotoh, D. E. Wazer, H. Band, L. A. Feig, and V. Band
The High-Risk Human Papillomavirus Type 16 E6 Counters the GAP Function of E6TP1 toward Small Rap G Proteins
J. Virol., December 20, 2002; 77(2): 1614 - 1620.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Liu, B. R. Schwartz, J. Tupper, N. Lin, R. K. Winn, and J. M. Harlan
The GTPase Rap1 Regulates Phorbol 12-Myristate 13-Acetate-stimulated but Not Ligand-induced beta 1 Integrin-dependent Leukocyte Adhesion
J. Biol. Chem., October 18, 2002; 277(43): 40893 - 40900.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
J. M. Martinez, C. A. Afshari, P. R. Bushel, A. Masuda, T. Takahashi, and N. J. Walker
Differential Toxicogenomic Responses to 2,3,7,8-Tetrachlorodibenzo-p-dioxin in Malignant and Nonmalignant Human Airway Epithelial Cells
Toxicol. Sci., October 1, 2002; 69(2): 409 - 423.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. Kang, H. Kae, H. Ip, G. B. Spiegelman, and G. Weeks
Evidence for a role for the Dictyostelium Rap1 in cell viability and the response to osmotic stress
J. Cell Sci., September 15, 2002; 115(18): 3675 - 3682.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Woulfe, H. Jiang, R. Mortensen, J. Yang, and L. F. Brass
Activation of Rap1B by Gi Family Members in Platelets
J. Biol. Chem., June 21, 2002; 277(26): 23382 - 23390.
[Abstract] [Full Text] [PDF]

Home page
 Cell Growth Differ.Home page
L. Lu, K. Holmqvist, M. Cross, and M. Welsh
Role of the Src Homology 2 Domain-containing Protein Shb in Murine Brain Endothelial Cell Proliferation and Differentiation
Cell Growth Differ., March 1, 2002; 13(3): 141 - 148.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Katagiri, M. Hattori, N. Minato, and T. Kinashi
Rap1 Functions as a Key Regulator of T-Cell and Antigen-Presenting Cell Interactions and Modulates T-Cell Responses
Mol. Cell. Biol., February 15, 2002; 22(4): 1001 - 1015.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Li, M. Okura, and A. Imamoto
Focal Adhesions Require Catalytic Activity of Src Family Kinases To Mediate Integrin-Matrix Adhesion
Mol. Cell. Biol., February 15, 2002; 22(4): 1203 - 1217.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. S. Zawistowski, I. G. Serebriiskii, M. F. Lee, E. A. Golemis, and D. A. Marchuk
KRIT1 association with the integrin-binding protein ICAP-1: a new direction in the elucidation of cerebral cavernous malformations (CCM1) pathogenesis
Hum. Mol. Genet., February 1, 2002; 11(4): 389 - 396.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
K. H. Shain and W. S. Dalton
Cell Adhesion Is a Key Determinant in de Novo Multidrug Resistance (MDR): New Targets for the Prevention of Acquired MDR
Mol. Cancer Ther., November 1, 2001; 1(1): 69 - 78.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. M. Schmitt and P. J. S. Stork
Cyclic AMP-Mediated Inhibition of Cell Growth Requires the Small G Protein Rap1
Mol. Cell. Biol., June 1, 2001; 21(11): 3671 - 3683.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
J. Garcia, J. de Gunzburg, A. Eychène, S. Gisselbrecht, and F. Porteu
Thrombopoietin-Mediated Sustained Activation of Extracellular Signal-Regulated Kinase in UT7-Mpl Cells Requires Both Ras-Raf-1- and Rap1-B-Raf-Dependent Pathways
Mol. Cell. Biol., April 15, 2001; 21(8): 2659 - 2670.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
A. Schmidt, E. Caron, and A. Hall
Lipopolysaccharide-Induced Activation of {beta}2-Integrin Function in Macrophages Requires Irak Kinase Activity, p38 Mitogen- Activated Protein Kinase, and the Rap1 GTPase
Mol. Cell. Biol., January 15, 2001; 21(2): 438 - 448.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
Y Sawada, K Nakamura, K Doi, K Takeda, K Tobiume, M Saitoh, K Morita, I Komuro, K De Vos, M Sheetz, et al.
Rap1 is involved in cell stretching modulation of p38 but not ERK or JNK MAP kinase
J. Cell Sci., January 3, 2001; 114(6): 1221 - 1227.
[Abstract] [PDF]

Home page
 Physiol. Rev.Home page
Y. Takai, T. Sasaki, and T. Matozaki
Small GTP-Binding Proteins
Physiol Rev, January 1, 2001; 81(1): 153 - 208.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Czyzyk, D. Leitenberg, T. Taylor, and K. Bottomly
Combinatorial Effect of T-Cell Receptor Ligation and CD45 Isoform Expression on the Signaling Contribution of the Small GTPases Ras and Rap1
Mol. Cell. Biol., December 1, 2000; 20(23): 8740 - 8747.
[Abstract] [Full Text]

Home page
 JCBHome page
K. A. Reedquist, E. Ross, E. A. Koop, R. M.F. Wolthuis, F. J.T. Zwartkruis, Y. van Kooyk, M. Salmon, C. D. Buckley, and J. L. Bos
The Small GTPase, Rap1, Mediates CD31-induced Integrin Adhesion
J. Cell Biol., March 20, 2000; 148(6): 1151 - 1158.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Katagiri, M. Hattori, N. Minato, S.-k. Irie, K. Takatsu, and T. Kinashi
Rap1 Is a Potent Activation Signal for Leukocyte Function-Associated Antigen 1 Distinct from Protein Kinase C and Phosphatidylinositol-3-OH Kinase
Mol. Cell. Biol., March 15, 2000; 20(6): 1956 - 1969.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
B. Franke, M. van Triest, K. M. T. de Bruijn, G. van Willigen, H. K. Nieuwenhuis, C. Negrier, J.-W. N. Akkerman, and J. L. Bos
Sequential Regulation of the Small GTPase Rap1 in Human Platelets
Mol. Cell. Biol., February 1, 2000; 20(3): 779 - 785.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J. de Rooij, H. Rehmann, M. van Triest, R. H. Cool, A. Wittinghofer, and J. L. Bos
Mechanism of Regulation of the Epac Family of cAMP-dependent RapGEFs
J. Biol. Chem., June 30, 2000; 275(27): 20829 - 20836.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Schmitt and P. J. S. Stork
beta 2-Adrenergic Receptor Activates Extracellular Signal-regulated Kinases (ERKs) via the Small G Protein Rap1 and the Serine/Threonine Kinase B-Raf
J. Biol. Chem., August 11, 2000; 275(33): 25342 - 25350.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Yamashita, N. Mochizuki, Y. Ohba, M. Tobiume, Y. Okada, H. Sawa, K. Nagashima, and M. Matsuda
CalDAG-GEFIII Activation of Ras, R-Ras, and Rap1
J. Biol. Chem., August 11, 2000; 275(33): 25488 - 25493.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Arai, Y. Nosaka, E. Kanda, K. Yamamoto, N. Miyasaka, and O. Miura
Rap1 Is Activated by Erythropoietin or Interleukin-3 and Is Involved in Regulation of beta 1 Integrin-mediated Hematopoietic Cell Adhesion
J. Biol. Chem., March 23, 2001; 276(13): 10453 - 10462.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Song, C.-D. Hu, M. Masago, K.-i. Kariya, Y. Yamawaki-Kataoka, M. Shibatohge, D. Wu, T. Satoh, and T. Kataoka
Regulation of a Novel Human Phospholipase C, PLCepsilon , through Membrane Targeting by Ras
J. Biol. Chem., January 19, 2001; 276(4): 2752 - 2757.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. J. Dupuy, K. Morgan, F. C. von Lintig, H. Shen, H. Acar, D. E. Hasz, N. A. Jenkins, N. G. Copeland, G. R. Boss, and D. A. Largaespada
Activation of the Rap1 Guanine Nucleotide Exchange Gene, CalDAG-GEF I, in BXH-2 Murine Myeloid Leukemia
J. Biol. Chem., April 6, 2001; 276(15): 11804 - 11811.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Liao, T. Satoh, X. Gao, T.-G. Jin, C.-D. Hu, and T. Kataoka
RA-GEF-1, a Guanine Nucleotide Exchange Factor for Rap1, Is Activated by Translocation Induced by Association with Rap1{middle dot}GTP and Enhances Rap1-dependent B-Raf Activation
J. Biol. Chem., July 20, 2001; 276(30): 28478 - 28483.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T.-G. Jin, T. Satoh, Y. Liao, C. Song, X. Gao, K.-i. Kariya, C.-D. Hu, and T. Kataoka
Role of the CDC25 Homology Domain of Phospholipase Cepsilon in Amplification of Rap1-dependent Signaling
J. Biol. Chem., August 3, 2001; 276(32): 30301 - 30307.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.