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J Biol Chem, Vol. 274, Issue 26, 18567-18573, June 25, 1999
From the Division of Biological Sciences, Graduate School of
Science, Hokkaido University, Sapporo 060-0810, Japan
We determined the complete nucleotide sequences
of the
Tandem Organization of Medaka Fish Soluble Guanylyl Cyclase
1 and 
1 Subunit Genes
IMPLICATIONS FOR COORDINATED TRANSCRIPTION OF TWO SUBUNIT
GENES
1 subunit gene
(OlGCS-1) and the 
1 subunit
gene (OlGCS-1) of medaka fish soluble
guanylyl cyclase. In the genome, OlGCS-1 and
OlGCS-1 are organized in tandem. The two
genes are only 986 base pairs apart and span approximately 34 kilobase
pairs in the order of OlGCS-1 and
OlGCS-1. The nucleotide sequence of a large
part of the 5'-upstream region of OlGCS-1 is
complimentarily conserved in that of
OlGCS-1. To analyze the promoter activity of
each gene, a fusion gene construct in which the 5'-upstream region was
fused with the green fluorescent protein gene was injected into medaka
fish 2-cell embryos. When the fusion gene containing the
OlGCS-1 upstream region was injected, green
fluorescent protein fluorescence was detected in the embryonic
brain. The 5'-upstream region of OlGCS-1
alone was insufficient for the reporter gene expression in the embryos.
When the OlGCS-1 upstream region was located
upstream of the OlGCS-1-green fluorescence
protein fusion gene, the reporter gene was expressed in the brain and
trunk region of the embryos. These results suggest that the
5'-upstream region of OlGCS-1 can
affect the expression of OlGCS-1. It is
therefore possible that the expression of
OlGCS-1 and OlGCS-1 is coordinated.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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